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Foreasy RNase Inhibitor
◮A new mouse-derived RNase inhibitor inoratidzwa muE. coli engineered mabhakitiriya achishandisa gene recombination technology.Iyo inhibitor inhibitor RNase chiitiko nekusava nemakwikwi kusanganisa neRNase 1: 1, nekudaro ichidzivirira.RNAkutendeseka, uye inogona kuvharidzira zvinobudiriratheRNase A, B, C chiitiko , asi kwete RNase T1, T2, H, nezvimwewo; Inodzoserwa kumashure kwekusungirirwa kwe RNase Inhibitor uye RNase, uye iyo yakaoma inogona kuparadzaniswa ne urea uye sulfhydryl reagents, kuitira kuti RNase renatures uye inhibitor isingagadziriswi inactivated.
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Mbizi Hove Yakananga PCR Kit-UNG Yakananga PCR Lysis Reagent(zebrafish)
Kushandiswa kwakananga kwezebrafish lysate se template yePCR amplification, hapana kubviswa kwakasiyana kweDNA, nekukurumidza uye kwakanyanya kunzwa.Yakawedzera UNG anti-kusvibisa sisitimu yekubvisa manyepo.
◮Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.
◮Sample inodiwa idiki, ingotora mhodzi imwe chete.
◮Hapana mishonga yakakosha yakadai sekugaya nekupwanya inodiwa, uye kushanda kuri nyore.
◮Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.
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Foreasy Taq DNA Polymerase
◮Yakanyanya kujeka: Iyo enzyme ine imwe inopisa-yekutanga chiitiko.
◮Kukurumidza Kuwedzera: 10 sec/kb.
◮Yakanyanya kuchinjika template: inogona kushandiswa kunyatso kusimudzira GC Yakakwirira kukosha, akasiyana-siyana akaoma-ku-kurisa DNA template.
◮Kuvimbika kwakasimba: yakajairwa Taq Enzyme 6 nguva.
◮Kugadzikana kwekushisa kwakasimba: Inogona kuiswa pa 37 ° C kwevhiki uye inochengetedza zvinopfuura 90% chiitiko.
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Foreasy HS Taq DNA Polymerase
◮Yakanyanya kujeka: Iyo enzyme ine yakanyanya kupisa-kutanga basa.
◮Kukurumidza Kuwedzera: 10 sec/kb.
◮Yakakwira template kuchinjika: inogona kushandiswa kunyatso kusimudzira YakakwiriraGCvalueuyezvakasiyana-siyana zvakaoma-ku-kurisa DNA template.
◮Kuvimbika kwakasimba: Kuvimbika ndiko 6 nguvaof zvakajairika Taq Enzyme.
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Animal Tissue Direct PCR kit (isina Sampling Zvishandiso) Protocol
Shandisa zvakananga mhuka yemhuka lysate se template yePCR amplification, pasina kubviswa kwakasiyana kweDNA, kukurumidza uye kunzwisisika kwakanyanya.
◮Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.
◮Sample inodiwa idiki, ingotora mhodzi imwe chete.
◮Hapana mishonga yakakosha yakadai sekugaya nekupwanya inodiwa, uye kushanda kuri nyore.
◮Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.
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Real Time PCR Easyᵀᴹ-Taqman
◮Nyore-2 × PCR Sanganisa kuderedza kuyedza kukanganisa uye nguva yekushandisa
◮Yakananga-yakagadziridzwa buffer uye inopisa-kutanga Taq enzyme inogona kudzivirira isina-chaiyo amplification uye primer dimer kuumbwa.
◮Kunzwa kwakanyanya-inogona kuona yakaderera makopi etemplate
◮Zvakanaka kuita zvakasiyana-siyana-inoenderana neyakawanda-chaiyo-nguva yakawanda PCR zviridzwa
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RT Easyᵀᴹ II Master Premix yekutanga-strand cDNA synthesis yeChaiyo Nguva PCR
-Inoshanda reverse transcription system, zvinongotora maminetsi makumi maviri kupedzisa kusanganiswa kweiyo yekutanga strand cDNA.
-Yakakwirira-sensitivity reverse transcription system, pg-level matemplate anogonawo kuwana yakakwirira-mhando cDNA.
-Iyo reverse transcription system ine yakanyanya kugadzikana yekupisa, tembiricha yemaitiro inogona kunge yakakwira kusvika 50 ℃, uye ine yakanaka reverse transcription performance.
-Kunyangwe masampuli eRNA asina kuchena (doro rinosvika 60%, guanidine munyu unosvika 750mM) inogona kuisirwa reverse transcription reaction.
- 2 × RT OR-Zviri nyoreTM Musanganiswa wakawedzera optimized reverse transcription primers (Random Primer, Oligo(dT)18 Primer).
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Ropa SuperDirectᵀᴹ PCR Kit-EDTA Ropa Rakananga PCR Master Mix yeGenotyping yeRopa.
Shandisa zvakananga EDTA anticoagulated ropa rose se template yePCR amplification, ine high sensitivity uye yakakwirira amplification kunyatsoshanda.
◮Hapana chikonzero chekutora nguva uye inodhura DNA kunatswa, hapana pretreatment, uye zvakananga kushandisa ropa rose sePCR template.
◮Iyo sisitimu ine simba rekusimudzira uye inogona kunyatsoona genome uye yekunze inonangwa zvidimbu zveDNA muropa.
◮2 × SuperEasyTMSanganisa-EDTA Kushivirira kweropa kwepamusoro: kusvika ku45% muropa revanhu uye 20% muropa rembeva.
◮Sample yakavharirwa zvizere, saka hapana chikonzero chekunetseka nezvekusvibiswa kwemuenzaniso uye nhema dzakanaka dzePCR.
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PCR Heroᵀᴹ (Nedhayi)
◮Kuvimbika kwepamusoro: 6 nguva yeyakajairika Taq enzyme;
◮Inokurumidza amplification speed
◮More template adaptability
◮Kukwirisa kwepamusoro kushanda zvakanaka
◮Kushivirira kwezvakatipoteredza kwakasimba: kuiswa pa 37 ° C kwevhiki, kuchengetedza zvinopfuura 90% basa;
◮Iine 5'→ 3' DNA polymerase chiitiko uye 5'→ 3' exonuclease chiitiko, isina 3'→ 5' exonuclease chiitiko.
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Ropa SuperDirectᵀᴹ PCR Kit-Heparin Ropa Yakananga PCR Master Mix yeGenotyping yeRopa
Shandisa zvakananga heparin anticoagulated ropa rose se template yePCR amplification, ine high sensitivity uye yakakwirira amplification kunyatsoshanda.
◮Hapana chikonzero chekutora nguva uye inodhura DNA kunatswa, hapana pretreatment, uye zvakananga kushandisa ropa rose sePCR template.
◮Iyo sisitimu ine simba rekusimudzira uye inogona kunyatsoona genome uye yekunze inonangwa zvidimbu zveDNA muropa.
◮2 × SuperEasyTMMix-Heparin High kushivirira kweropa: kusvika ku45% muropa revanhu uye 20% muropa rembeva.
◮Sample yakavharirwa zvizere, saka hapana chikonzero chekunetseka nezvekusvibiswa kwemuenzaniso uye nhema dzakanaka dzePCR.
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Ropa SuperDirectᵀᴹ PCR Kit(UNG)-Heparin Ropa Yakananga PCR Master Mix yeGenotyping yeRopa
Shandisa zvakananga heparin anticoagulated ropa rose se template yePCR amplification, ine high sensitivity uye yakakwirira amplification kunyatsoshanda.Iyo UNG anti-pollution system yakawedzerwa kudzivirira maitiro enhema.
◮Iyo sisitimu ine simba rekusimudzira uye inogona kunyatsoona genome uye yekunze inonangwa zvidimbu zveDNA muropa.
◮2 × SuperEasyTMMix (UNG) -Heparin Kushivirira kweropa: kusvika ku45% muropa revanhu uye 20% muropa rembeva.
◮Sample yakavharirwa zvizere, saka hapana chikonzero chekunetseka nezvekusvibiswa kwemuenzaniso uye nhema dzakanaka dzePCR.Anti-kusvibiswa PCR system
◮2 × SuperEasyTMSanganisa (UNG), nyatso bvisa kusvibiswa kunokonzerwa nePCR zvigadzirwa, uye simbisa iyo chaiyo uye nekururama kweamplification.
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Ropa SuperDirectᵀᴹ PCR Kit(UNG)-EDTA Ropa Rakananga PCR Master Mix yeGenotyping yeRopa
Shandisa zvakananga EDTA anticoagulated ropa rose se template yePCR amplification, ine high sensitivity uye yakakwirira amplification kunyatsoshanda.Iyo UNG anti-pollution system yakawedzerwa kudzivirira maitiro enhema.
◮Hapana chikonzero chekutora nguva uye inodhura DNA kunatswa, hapana pretreatment, uye zvakananga kushandisa ropa rose sePCR template.
◮Iyo sisitimu ine simba rekusimudzira uye inogona kunyatsoona genome uye yekunze inonangwa zvidimbu zveDNA muropa.
◮2 × SuperEasyTMMix(UNG) -EDTA ine kushivirira kweropa kwakakwira: kusvika ku45% muropa revanhu uye 20% muropa rembeva.
◮Sample yakavharirwa zvizere, saka hapana chikonzero chekunetseka nezvekusvibiswa kwemuenzaniso uye nhema dzakanaka dzePCR.Anti-kusvibiswa PCR system
◮2 × SuperEasyTMMix(UNG) -EDTA, inobvisa zvinobudirira kusvibiswa kunokonzerwa nePCR zvigadzirwa, uye inova nechokwadi chekunyatsojeka uye kurongeka kweamplification.
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