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Mouse Muswe SuperDirect PCR Kit Yakananga PCR Lysis Reagent(Mouse Muswe)(yeGenotyping)
Shandisa zvakananga mbeva muswe lysate se template yePCR amplification, isina kubviswa kwakasiyana kweDNA, nekukurumidza uye kwakanyanya kunzwa.
◮Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.
◮Sample inodiwa idiki, ingotora mhodzi imwe chete.
◮Hapana mishonga yakakosha yakadai sekugaya nekupwanya inodiwa, uye kushanda kuri nyore.
◮Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.
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Animal Tissue Direct PCR kit-UNG (isina Sampling Zvishandiso) Protocol
Shandisa zvakananga mhuka yemhuka lysate se template yePCR amplification, pasina kubviswa kwakasiyana kweDNA, kukurumidza uye kunzwisisika kwakanyanya.Yakawedzera UNG anti-kusvibisa sisitimu yekubvisa manyepo.
◮Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.
◮Sample inodiwa idiki, ingotora mhodzi imwe chete.
◮Hapana mishonga yakakosha yakadai sekugaya nekupwanya inodiwa, uye kushanda kuri nyore.
◮Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.
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Mouse Muswe Wakananga PCR Kit Yakananga PCR Lysis Reagent (Mouse Muswe) (yeGenotyping)
Shandisa zvakananga mbeva muswe lysate se template yePCR amplification, isina kubviswa kwakasiyana kweDNA, nekukurumidza uye kwakanyanya kunzwa.
◮Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.
◮Sample inodiwa idiki, ingotora mhodzi imwe chete.
◮Hapana mishonga yakakosha yakadai sekugaya nekupwanya inodiwa, uye kushanda kuri nyore.
◮Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.
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Mouse Muswe Wakananga PCR Kit-UNG Yakananga PCR Lysis Reagent(Mouse Muswe)(yeGenotyping)
Iyo mbeva muswe lysate inoshandiswa zvakananga se template yePCR kukwidziridzwa pasina yakaparadzana kudhirowa kweDNA, iyo inokurumidza uye yakanyanya kunzwa.Yakawedzera UNG anti-kusvibisa sisitimu yekubvisa manyepo.
◮Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.
◮Sample inodiwa idiki, ingotora mhodzi imwe chete.
◮Hapana mishonga yakakosha yakadai sekugaya nekupwanya inodiwa, uye kushanda kuri nyore.
◮Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.
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Mbizi Hove Yakananga PCR Kit Yakananga PCR Lysis Reagent (mbizi)
Shandisa zvakananga zebrafish lysate se template yePCR amplification, pasina kubviswa kwakasiyana kweDNA, inokurumidza uye yakanyanya kunzwa.
◮Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.
◮Sample inodiwa idiki, ingotora mhodzi imwe chete.
◮Hapana mishonga yakakosha yakadai sekugaya nekupwanya inodiwa, uye kushanda kuri nyore.
◮Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.
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Dyara shizha Direct PCR Plus kit (isina Sampling Zvishandiso) Protocol Direct PCR kubva kuPlat Material.
Shandisa zvakananga polysaccharide uye polyphenol leaf lysate se template yePCR amplification, pasina kubviswa kwakasiyana kweDNA, inokurumidza uye yakakwirira senitivity.
◮Hapana kutora nguva uye kudhura kweDNA kucheneswa (Nguva, basa uye mutengo wakachengetedzwa!)
◮Zvinhu zvishoma
◮Nyore-sample inogona kuiswa pasi pePCR reaction kana lysis reaction pasina kucheka kana kugaya
◮2 X Musanganiswa-kuderedza zvikanganiso zvekurodha sampuli uye maitiro ekugadzirisa system nguva
◮Fast-template kugadzirira inogona kupedzwa mumaminitsi gumi, kuita kwePCR kunogona kupedzwa mumaminitsi makumi mashanu
◮High throughput-lysis reaction inogona kupedzwa mu96-tsime PCR ndiro
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Dyara Mbeu Yakananga PCR Kit I/II (isina Sampling Zvishandiso) Protocol Plant Direct PCR Kits
◮Hapana kutora nguva uye kudhura kweDNA kucheneswa (Nguva, basa uye mutengo wakachengetedzwa!)
◮Zvinhu zvishoma
◮Nyore-sample inogona kuiswa pasi pePCR reaction kana lysis reaction pasina kucheka kana kugaya
◮2 X Musanganiswa-kuderedza zvikanganiso zvekurodha sampuli uye maitiro ekugadzirisa system nguva
◮Fast-template kugadzirira inogona kupedzwa mumaminitsi gumi, kuita kwePCR kunogona kupedzwa mumaminitsi makumi mashanu
◮High throughput-lysis reaction inogona kupedzwa mu96-tsime PCR ndiro
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Dyara shizha Direct PCR pamwe nekiti-UNG (isina Sampling Zvishandiso) Protocol Direct PCR kubva kuPlat Material.
Shandisa zvakananga polysaccharide uye polyphenol leaf lysate se template yePCR amplification, pasina kubviswa kwakasiyana kweDNA, inokurumidza uye yakakwirira senitivity.Iyo UNG anti-pollution system yakawedzerwa kudzivirira maitiro enhema.
◮Hapana kutora nguva uye kudhura kweDNA kucheneswa (Nguva, basa uye mutengo wakachengetedzwa!)
◮Zvinhu zvishoma
◮Nyore-sample inogona kuiswa pasi pePCR reaction kana lysis reaction pasina kucheka kana kugaya
◮2 X Musanganiswa-kuderedza zvikanganiso zvekurodha sampuli uye maitiro ekugadzirisa system nguva
◮Fast-template kugadzirira inogona kupedzwa mumaminitsi gumi, kuita kwePCR kunogona kupedzwa mumaminitsi makumi mashanu
◮High throughput-lysis reaction inogona kupedzwa mu96-tsime PCR ndiro
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RT-qPCR Easyᵀᴹ (Imwe Danho)-Taqman
- Poshi-nhanho kit inogonesa reverse transcription uye PCR kuti iitwe mune imwechete chubhu, inongoda kuwedzera template RNA, chaiyo PCR primers uye RNase-Mahara ddH.2O.
-Chaiyo-nguva yehuwandu kuongororwa kwehutachiona RNA kana kutsvaga RNA inogona kuitwa nekukurumidza uye nemazvo.
-Iyo kit inoshandisa yakasarudzika Foregene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
- Iyo yakagadziridzwa maitiro system inoita kuti maitiro ave nepamusoro ekuona senitivity, kusimba kwekushisa kwekushisa, uye kushivirira kuri nani.
- RT-qPCR NyoreTM(Imwe Danho) -Taqman kit inouya neROX yemukati referensi dhayi, iyo inogona kushandiswa kubvisa chiratidzo chekumashure uye zvikanganiso zvechiratidzo pakati pematsime, izvo zviri nyore kune vashandisi vekupedzisira kushandisa mumhando dzakasiyana dzehuwandu hwePCR zviridzwa.
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RNAlater (YeRNA Kudzikamisa) RNAlater Kudzikamisa Solution
Kukurumidza kugadzirisa kwezvichangobva kutorwa mhuka dzemuenzaniso tishu, inhibit RNase chiitiko, chengetedza RNA kubva mukuora.
-Reagents anoshandiswa zvakananga, nhanho imwe iripo, uye RNA inokurumidza kudzikamiswa uye kuchengetedzwa.
-Kushanda pakupisa kwekamuri, kuri nyore, kwakachengeteka uye kusina chepfu.
-Kuti ubvise dambudziko rechando chakaoma kana firiji yepasi-tembiricha yekuchengetedza tishu.
-Iyo matishu inogona kuchengetwa kwenguva yakareba pasina njodzi yekuparara kweRNA, kuve nechokwadi chekuvimbika gene expression profile data.
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PCR Heroᵀᴹ
◮Kuvimbika kwepamusoro: 6nguva iyo yeyakajairika Taq enzyme;
◮Kukurumidza akuwedzera kumhanya:iyo amplification kumhanya ndeye 5-10sec/kb, inova 6-12 nguva yeyakajairika Taq enzyme;
◮More template adaptability: inogona kunyatso kusimudzira akasiyana akaomesesa eDNA matemplate ane yakakwira GC zvemukati uye yakaoma kukudza;
◮HigherAmplification kunyatsoshanda: nhamba yeamplification cycles yakaderera pane iyo yakajairwa Taq enzyme;
◮Syakasimba Kushivirira kwezvakatipoteredza: yakaiswa pa 37°C kwevhiki, ichichengetedza kupfuura90%chiitiko;
◮Iine 5'→ 3' DNA polymerase chiitiko uye 5'→ 3' exonuclease chiitiko, isina 3'→ 5' exonuclease chiitiko.
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PCR Easyᵀᴹ
Iyo yakasarudzika maitiro sisitimu uye yakakwira-inoshanda Taq DNA Polymerase inoita iyo PCR maitiro ive nepamusoro amplification kunyatsoita, kujeka uye kunzwa.
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