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RT-qPCR Easyᵀᴹ (Imwe Danho) -SYBR Green I
◮Imwe-nhanho kit inoita reverse transcription uye qPCR maitiro maviri muchubhu imwechete, inongoda kuwedzera template RNA, chaiyo PCR primers uye RNase-Mahara ddH.2O.
◮Iyo kit inogona nekukurumidza uye zvine hungwaru kuongorora hutachiona RNA kana kutsvaga RNA.
◮Iyo kit inoshandisa yakasarudzika yeForegene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
◮Iyo optimized reaction system inoita kuti mhinduro ive nepamusoro yekuona senitivity, kusimba kwekupisa kwekushisa, uye kushivirira kuri nani.
◮RT-qPCR NyoreTM(Imwe Danho) -SYBR Green I kit inouya neROX yemukati referensi dhayi, iyo inogona kushandiswa kubvisa chiratidzo chekumashure uye zvikanganiso zvechiratidzo pakati pematsime, izvo zviri nyore kuti vatengi vashandise mumhando dzakasiyana dzehuwandu hwePCR zviridzwa.
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RT-qPCR Easyᵀᴹ (Imwe Danho)-Taqman
- Poshi-nhanho kit inogonesa reverse transcription uye PCR kuti iitwe mune imwechete chubhu, inongoda kuwedzera template RNA, chaiyo PCR primers uye RNase-Mahara ddH.2O.
-Chaiyo-nguva yehuwandu kuongororwa kwehutachiona RNA kana kutsvaga RNA inogona kuitwa nekukurumidza uye nemazvo.
-Iyo kit inoshandisa yakasarudzika Foregene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
- Iyo yakagadziridzwa maitiro system inoita kuti maitiro ave nepamusoro ekuona senitivity, kusimba kwekushisa kwekushisa, uye kushivirira kuri nani.
- RT-qPCR NyoreTM(Imwe Danho) -Taqman kit inouya neROX yemukati referensi dhayi, iyo inogona kushandiswa kubvisa chiratidzo chekumashure uye zvikanganiso zvechiratidzo pakati pematsime, izvo zviri nyore kune vashandisi vekupedzisira kushandisa mumhando dzakasiyana dzehuwandu hwePCR zviridzwa.
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