PCR Heroᵀᴹ (Nedhayi)
Kit Description:
◮Kuvimbika kwepamusoro: 6 nguva yeyakajairika Taq enzyme;
◮Inokurumidza amplification speed
◮More template adaptability
◮Kukwirisa kwepamusoro kushanda zvakanaka
◮Kushivirira kwezvakatipoteredza kwakasimba: kuiswa pa 37 ° C kwevhiki, kuchengetedza zvinopfuura 90% basa;
◮Iine 5'→ 3' DNA polymerase chiitiko uye 5'→ 3' exonuclease chiitiko, isina 3'→ 5' exonuclease chiitiko.
Zvinotsanangurwa
5ml, 10ml, 40ml
PCR Gamba (NeDye) Yekuwedzera kit inosanganisira yeForegene yakasarudzika chizvarwa chitsva cheTaq-Foregene Taq Gamba, yakagadziridzwa dNTPs, MgCl2, reaction buffer, PCR reaction inosimudzira, optimizer uye stabilizer.Iyo 2 × PCR Hero TM Musanganiswa (dhayi) system ine kushivirira kwakanyanya kune PCR inhibitors kupfuura yakajairwa PCR Mix system, uye inogona kutarisana nyore nePCR kukwidziridzwa kweakasiyana matemplate akaoma.Iyo yakasarudzika maitiro sisitimu uye yakakwirira-inoshanda Taq Gamba inoita kuti PCR iite ive nepamusoro amplification kunyatsoita, kujeka uye kunzwa.
Kit zvikamu
| 0.1U Taq Gamba/μl |
| 400μM dNTPs |
| 20mM Tris-HCl(pH8.8) |
| 100mM KCl |
| 5mM MgCl2 |
| Zvimwe zvinosimbisa uye zvinowedzera |
| DNA Loading Buffer |
Zvimiro&zvakanakira
■Kuvimbika kwepamusoro: 6 nguva yeyakajairika Taq enzyme;
■ Inokurumidza amplification sipidhi : sipidhi yeamplification ndeye 5-10sec/kb, inova ka6-12 kakapetwa ka12 kune yakajairika Taq enzyme;
■ Kuwedzera kuchinjika kwetemplate: Inogona kukudza zvakasiyana-siyana zvakaoma matemplate eDNA nepamusoroGC zvemukati;
∎ Kukwirisa kwepamusoro: mitsetse yekusimudzira yakaderera pane yakajairika Taq enzyme;
■ Kushivirira kwezvakatipoteredza kwakasimba: kuiswa pa37°C kwevhiki, kuchengetedza zvinopfuura 90% basa;
■ Ine 5'→3'DNA polymerase chiitiko uye 5'→3'exonuclease chiitiko, pasina 3'→5'exonuclease chiitiko.
■ Iyo kititi yakagara yakasanganiswa neElectrophoresis Loading Buffer, mushure mokunge PCR yapera, sampu inogona kutakurwa zvakananga kune electrophoresis;kushanda kuri nyore uye nyore.
Kit application
PCR kukwidziridzwa kwezvimedu zveDNA, kunyora DNA, DNA sequencing,PCR pamwe nemuswe
Storage uye Sherufu upenyu
Inogona kuchengetwa kwenguva yakareba pa -20°C;kuti ishandiswe kakawanda, inogona kuchengetwa pa4°C kwenguva pfupi.
