Ropa SuperDirectᵀᴹ PCR Kit-Heparin Ropa Yakananga PCR Master Mix yeGenotyping yeRopa
Kit Description:
Shandisa zvakananga heparin anticoagulated ropa rose se template yePCR amplification, ine high sensitivity uye yakakwirira amplification kunyatsoshanda.
◮Hapana chikonzero chekutora nguva uye inodhura DNA kunatswa, hapana pretreatment, uye zvakananga kushandisa ropa rose sePCR template.
◮Iyo sisitimu ine simba rekusimudzira uye inogona kunyatsoona genome uye yekunze inonangwa zvidimbu zveDNA muropa.
◮2 × SuperEasyTMMix-Heparin High kushivirira kweropa: kusvika ku45% muropa revanhu uye 20% muropa rembeva.
◮Sample yakavharirwa zvizere, saka hapana chikonzero chekunetseka nezvekusvibiswa kwemuenzaniso uye nhema dzakanaka dzePCR.
Zvinotsanangurwa
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMMix-Heparin:Ine yakagadziridzwa D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer, stabilizer, nezvimwewo2O kune inoenderana 2 × SuperEasyTMMix-Heparin kuti ishandiswe muPCR reaction.
6 × DNA Inorodha Buffer: Iyo Inorodha Buffer haina SDS.Zvinokurudzirwa kushandisa 6 × DNA Loading Buffer inosanganisirwa nekiti paunenge uchiita agarose gel electrophoresis kuitira kuti uwane yakanaka electrophoresis mhinduro.Usashandise Loading Buffer ine SDS, zvikasadaro pachava nemuswe wakakura wechiedza chakajeka munzira panguva ye electrophoresis, izvo zvinokanganisa mhedzisiro yekuedza.
Kit zvikamu
| 2 × SuperEasyTM Mix-Heparin |
| 6 × DNA Loading Buffer |
| Mirayiridzo |
Zvimiro&zvakanakira
-Hapana kudikanwa kwekutora nguva uye kudhura kweDNA kucheneswa, hapana pretreatment, uye kushandiswa kwakananga kweropa rose sePCR template.
-Iyo sisitimu ine simba rekusimudzira uye inogona kunyatsoona iyo genome uye yekune dzimwe nyika chinangwa cheDNA zvimedu muropa.
-2 × SuperEasyTMMix-Heparin High kushivirira kweropa: kusvika ku45% muropa revanhu uye 20% muropa rembeva.
-Sample yakavharirwa zvizere, saka hapana chikonzero chekunetseka nezvekusvibiswa kwemuenzaniso uye nhema dzakanaka dzePCR.
Kit application
Yakatsaurirwa kunanga PCR kuzivikanwa kweHeparin anti-coagulated ropa rose.(Kusanganisira: ropa remunhu, ropa rembeva, ropa rehuku, ropa reshiri, ropa remombe, ropa rembwa, nezvimwewo)
Workflow
Storage uye Sherufu upenyu
Iyi kit inofanira kuchengetwa pa -20 ℃.
2 × SuperEasyTMMix-Heparin，Kana ikashandiswa kakawanda, inogona kuchengetwa pa4°C kuitira kuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi).
6 × DNA Loading Buffer, Inogona kuchengetwa pa4 ° C kana -20 ° C kwenguva yakareba.
