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Real Time PCR Easyᵀᴹ-SYBR Green I kit
Nyore-2X PCR Sanganisa kuderedza kuyedza kukanganisa uye nguva yekushandisa
Yakananga-yakagadziridzwa buffer uye inopisa-kutanga Taq enzyme inogona kudzivirira isina-chaiyo amplification uye primer dimer kuumbwa.
Kunzwa kwakanyanya-inogona kuona yakaderera makopi etemplate
Zvakanaka kuita zvakasiyana-siyana-inoenderana neyakawanda-chaiyo-nguva yakawanda PCR zviridzwa
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RT-PCR Easyᵀᴹ I (Imwe Danho)
◮Iyo imwe-nhanho kit inogonesa reverse transcription uye PCR kuti iitwe muhubhu imwechete.Inongoda kuwedzera template RNA, chaiyo PCR primers uye RNase-Yemahara ddH2O.
◮Real-time quantitative analysis yeRNA inogona kuitwa nekukurumidza uye nemazvo.
◮Iyo kit inoshandisa yakasarudzika yeForegene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
◮Iyo optimized reaction system inoita kuti mhinduro ive nepamusoro yekuona senitivity, kusimba kwekupisa kwekushisa, uye kushivirira kuri nani.
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RT-PCR Easyᵀᴹ II(Nhanho mbiri)
◮Real-time quantitative analysis yeRNA inogona kuitwa nekukurumidza uye nemazvo.
◮Iyo kit inoshandisa yakasarudzika yeForegene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
◮ Iyo optimized reaction system inoita kuti mhinduro ive nepamusoro yekuona senitivity, kusimba kwekupisa kwekushisa, uye kushivirira kuri nani.
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Real Time PCR Easyᵀᴹ-Taqman
◮Nyore-2 × PCR Sanganisa kuderedza kuyedza kukanganisa uye nguva yekushandisa
◮Yakananga-yakagadziridzwa buffer uye inopisa-kutanga Taq enzyme inogona kudzivirira isina-chaiyo amplification uye primer dimer kuumbwa.
◮Kunzwa kwakanyanya-inogona kuona yakaderera makopi etemplate
◮Zvakanaka kuita zvakasiyana-siyana-inoenderana neyakawanda-chaiyo-nguva yakawanda PCR zviridzwa
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PCR Heroᵀᴹ (Nedhayi)
◮Kuvimbika kwepamusoro: 6 nguva yeyakajairika Taq enzyme;
◮Inokurumidza amplification speed
◮More template adaptability
◮Kukwirisa kwepamusoro kushanda zvakanaka
◮Kushivirira kwezvakatipoteredza kwakasimba: kuiswa pa 37 ° C kwevhiki, kuchengetedza zvinopfuura 90% basa;
◮Iine 5'→ 3' DNA polymerase chiitiko uye 5'→ 3' exonuclease chiitiko, isina 3'→ 5' exonuclease chiitiko.
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700bp DNA Ladder
◮700bp DNA Ladder chigadzirwa chakagadzirira-kushandisa uye chatova ne1x Loading Buffer.Iwe unogona kutora zvakananga huwandu hwakakodzera hweMucherechedzo we electrophoresis zvinoenderana nekuyedza zvinodiwa.
700bpDNA Mucherechedzo Ⅰ ine 7 DNA mabhendi, iwo DNA mabhendi anoti: 100bp (50ng/5μl), 200bp (30ng/5μl), 300bp (30ng/5μl), 400bp (40ng/5μl), 500bp (500ng/50) , 500bp (20b/50) bp(35ng/5μl).
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2000bp DNA Ladder
◮ 2000bp DNA Ladder chigadzirwa chakagadzirira-kushandisa uye chatova ne1x Loading Buffer.Iwe unogona kutora zvakananga huwandu hwakakodzera hweMucherechedzo we electrophoresis zvinoenderana nekuyedza zvinodiwa.BM2000 DNA marker Chinerisation ye 6 DNA Bands, Bands
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5000bp DNA Ladder
◮ 2000bp DNA Ladderchigadzirwa chakagadzirira-kushandisa uye chatova ne1x Loading Buffer.Iwe unogona kutora zvakananga huwandu hwakakodzera hweMucherechedzo we electrophoresis zvinoenderana nekuyedza zvinodiwa.BM5000 DNA Marker ine 8 DNA mabhendi, mabhandi eDNA ndeaya: 100bp(50ng/5μl), 250bp(50ng/5μl), 500bp(50ng/5μl), 750bp(75ng/5μl), 1000)ng0/0b/5μl, 1000)ng0/5/5p(l) ), 3000bp(30ng/5μl), 5000bp(50ng/5μl).
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PCR Easyᵀᴹ (ine dhayi)
Iyo 2 × PCR GambaTM Mix system ine kushivirira kwepamusoro kune PCR inhibitors pane yakajairwa PCR Mix system, uye inogona kutarisana zviri nyore nePCR kukwidziridzwa kwematemplate akasiyana akaomarara.Iyo yakasarudzika maitiro sisitimu uye yakakwirira-inoshanda Taq Gamba inoita kuti PCR iite ive nepamusoro amplification kunyatsoita, kujeka uye kunzwa.
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PCR Easyᵀᴹ
Iyo yakasarudzika maitiro sisitimu uye yakakwira-inoshanda Taq DNA Polymerase inoita iyo PCR maitiro ive nepamusoro amplification kunyatsoita, kujeka uye kunzwa.
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PCR Heroᵀᴹ
◮Kuvimbika kwepamusoro: 6nguva iyo yeyakajairika Taq enzyme;
◮Kukurumidza akuwedzera kumhanya:iyo amplification kumhanya ndeye 5-10sec/kb, inova 6-12 nguva yeyakajairika Taq enzyme;
◮More template adaptability: inogona kunyatso kusimudzira akasiyana akaomesesa eDNA matemplate ane yakakwira GC zvemukati uye yakaoma kukudza;
◮HigherAmplification kunyatsoshanda: nhamba yeamplification cycles yakaderera pane iyo yakajairwa Taq enzyme;
◮Syakasimba Kushivirira kwezvakatipoteredza: yakaiswa pa 37°C kwevhiki, ichichengetedza kupfuura90%chiitiko;
◮Iine 5'→ 3' DNA polymerase chiitiko uye 5'→ 3' exonuclease chiitiko, isina 3'→ 5' exonuclease chiitiko.
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QuickEasyᵀᴹ Real Time PCR Kit-Taqman
◮Iyo yakasarudzika PCR optimization system inoita 2 × QuickEasyTMReal PCR Mix-Taqman inoenderana zvakanyanya.
◮Kupisa-kutanga Foregene HS Taq Polymerase ine yakakwirira kukwidziridza kunyatsoita, yakakwirira amplification senitivity, uye yakakwirira amplification chaiyo.
◮2 × QuickEasyTMReal PCR Mix -Taqman inoshandisa yakasarudzika sisitimu yakagadziridzwa neForegene kuvandudza kunzwisiswa uye kujeka kwekutevedzana-chaiyo probe yekuona, iyo inogona kushandiswa genotyping uye kukopa nhamba kusiyanisa kusarudza uye kuwana mhedzisiro chaiyo.
◮Ichi chigadzirwa chinouya neROX yemukati referensi dhayi, iyo inogona kushandiswa kubvisa chiratidzo chekumashure uye chiratidzo chikanganiso pakati pematsime, ayo ari nyore kuti vatengi vashandise mumhando dzakasiyana dzehuwandu hwePCR zviridzwa.
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