Cell Yese RNA Isolate Kit

Cell Yese RNA Isolate Kit

Tsananguro yeKit:

Yese RNA inogona kucheneswa mu11 mins.

Magadzirirwo:  50 Preps, 200 Preps

Mamiriro:

-Iyo nzira yese inoitwa panopisa tembiricha (15-25 ℃), isina bhati yechando uye yakadzika tembiricha centrifugation.

-Iyo kit kit yese RNase-Yemahara, hapana chikonzero chekushushikana nezve RNA kudzikisira.

-DNA-Kuchenesa Column inonyatso kusunga DNA, kuitira kuti kit igone kubvisa genomic DNA kusvibiswa pasina kuwedzera imwe DNase.

 

 

 

 


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  • Chigadzirwa Chigadzirwa

    Chigadzirwa Mateki

    Tsananguro

    Iyi kit inoshandisa spin kholamu uye fomula yakagadzirwa na Foregene, iyo inogona kunyatsobvisa yakachena-uye yepamusoro-soro yakazara RNA kubva kumaseru akagadzirwa mu96, 24, 12, uye 6-tsime mahwendefa.

    Iyo kitsi inopa inoshanda DNA-Kuchenesa Column, iyo inogona kupatsanura zviri nyore iyo supernatant uye cell lysate, kusunga uye kubvisa genomic DNA. Iko kuvhiya kuri nyore uye kunoponesa nguva.

    Tiye RNA-chete Column inogona kunyatso kusunga RNA ine yakasarudzika fomula. Nhamba huru yemasampuli inogona kugadziriswa panguva imwe chete.

    Kit zvikamu

    Kit kuumbwa RE-03111 RE-03114
    50 T 200 T
    Buffer cRL1 * 25 ml 100 mL
    Buffer cRL2 15 mL 60 mL
    Buffer RW1 * 25 ml 100 mL
    Buffer RW2 24 mL 96 mL
    RNase-Yemahara ddH2O 10 mL 40 mL
    RNA-Chete Column 50 200
    DNA-Yekuchenesa Column 50 200
    Mirayiridzo 1 1

    * Ndokumbirawo upfeke magurovhosi uye utore matanho ekudzivirira panguva yekushanda seBuffer cRL1 neBuffer RW1 ine zvinogumbura chaotropic munyu.

    Zvimiro & zvakanakira

    ■ Maitiro ese anoshandiswa panopisa tembiricha (15-25 ℃), isina bhati yechando uye tembiricha yakadzika.
    ■ Iyo kitu yese ndeye RNase-Yemahara, hapana chikonzero chekushushikana nezve RNA kudzikisira.
    ■ DNA-Yekuchenesa Column inonyatso kusunga DNA, kuitira kuti kit igone kubvisa genomic DNA kusvibiswa pasina kuwedzera imwe DNase.
    ■ Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatsochenesa RNA.
    ■ Kumhanyisa mhanyisa: nyore kushandisa uye inogona kupedzwa mumaminetsi gumi nerimwe.
    ■ Kuchengetedzwa: Hapana reagent yakasikwa inodikanwa.
    ■ Yepamusoro mhando: Iyo yakanatswa RNA ndeye yakachena kwazvo, isina mapuroteni nezvimwe zvakasviba, uye inogona kusangana akasiyana siyana anotevera ekuedza.

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    Kit chishandiso

    Iyo inokodzera kuburitsa uye kuchenesa yakazara RNA kubva kumaseru akakwenenzverwa mu96, 24, 12, uye 6-tsime mahwendefa.

    Kuyerera kwebasa

    cell total RNA

    Dhayagiramu

    Cell Total RNA Isolation Kit Work Flow1

    Iyo agarose gel bhatiri dhayagiramu yeCell Yese RNA Isolate Kit yakabata manhamba akasiyana pamusoro apa emaseru, 20μl vhoriyamu elution, tora 2μl yakanatswa yakazara RNA 1%.

    Kuchengetedza uye pasherufu hupenyu

    Iyo kit inokwanisa kuchengetwa kwemwedzi gumi nemaviri pakushisa kwemukati (15-25 ℃) kana 2-8 ℃ kwenguva yakareba (mwedzi makumi maviri nemana).

    Buffer cRL1 inogona kuchengetwa pa4 ℃ kwemwedzi mumwe mushure mekuwedzera 2-hydroxy-1-ethanethiol (sarudzo).


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    Chigadzirwa Mapoka