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Sero Yese RNA Isolation Kit Yese RNA Isolation Purification Kits kubva muchitokisi

Kit Description:

Yakanyanya kucheneswa uye yemhando yepamusoro RNA inogona kuwanikwa kubva kune akasiyana siyana maseru mumaminetsi gumi nerimwe.

RNase-Yemahara

Inoshanda pakamuri tembiricha (15-25 ℃)

NeDNA-Kuchenesa Column

High RNA goho

Kutsanya:Pedzisa kudhirowa mumaminitsi gumi nerimwe

Kuchengetedza: Hapana Organic makemikari

foregene simba


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  • Product Detail

    Product Tags

    FAQ

    Tsanangudzo

    Iyi kit inoshandisa spin column uye fomula yakagadziridzwa naForegene, iyo inogona kunyatso kuburitsa yakakwirira-kuchena uye yemhando yepamusoro RNA kubva kumaseru akagadzirwa mu96, 24, 12, uye 6-matsime mahwendefa.

    Iyo kit inopa inoshanda DNA-Kuchenesa Column, iyo inogona nyore kuparadzanisa supernatant uye sero lysate, kusunga uye kubvisa genomic DNA.Kuvhiya kuri nyore uye kunochengetedza nguva.

    Tiye RNA-chete Column anogona kunyatsosunga RNA neyakasiyana fomula.Nhamba huru yemasampuli inogona kugadziriswa panguva imwe chete.

    Kit zvikamu

    Kit composition RE-03111 RE-03114
    50 T 200 T
    Buffer cRL1* 25 mL 100 mL
    Buffer cRL2 15 mL 60 mL
    Buffer RW1* 25 mL 100 mL
    Buffer RW2 24 mL 96 mL
    RNase-Yemahara ddH2O 10 mL 40 mL
    RNA-Chete Column 50 200
    DNA-Kuchenesa Column 50 200
    Instruction 1 1

    * Ndokumbira upfeke magirovhosi uye tora matanho ekudzivirira panguva yekushanda sezvo Buffer cRL1 uye Buffer RW1 ine inogumbura chaotropic munyu.

    Zvimiro&zvakanakira

    ∎ Iyo yese nzira inoshandiswa pakudziya kwekamuri (15-25 ℃), pasina kugeza echando uye yakaderera tembiricha centrifugation.
    ■ Kiti yese ndeyeRNase-Yemahara, hapana chikonzero chekunetseka nezveRNA kuparara.
    ■ DNA-Cleaning Column inosunga zvakananga DNA, kuitira kuti kit ikwanise kubvisa genomic DNA kusvibiswa pasina kuwedzera imwe DNase.
    ■ Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatsochenesa RNA.
    ■ Kukurumidza kumhanya: nyore kushanda uye inogona kupedzwa mumaminitsi gumi nerimwe.
    ■ Chengetedzo: Hapana organic reagent inodiwa.
    ■ Hunhu hwepamusoro: RNA yakacheneswa ndeyekuchena kwepamusoro, haina mapuroteni uye kumwe kusvibiswa, uye inogona kusangana nemiedzo yakasiyana-siyana inotevera.

    zvakanakira foregene RNA Isolation kit

    Kit application

    Yakakodzera kudhirowa uye kucheneswa kweiyo RNA yakazara kubva kumasero akagadzirwa mu96, 24, 12, uye 6-matsime mahwendefa.

    Kuyerera kwebasa

    yesero yakazara RNA

    Mufananidzo

    Sero Yese RNA Isolation Kit Work Flow1

    Iyo agarose gel bhatiri dhayagiramu yeSero Yese RNA Isolation Kit yakabata nhamba dziri pamusoro dzakasiyana dzemasero, 20μl vhoriyamu elution, tora 2μl yakacheneswa yakazara RNA 1%.

    Kuchengetedza uye hupenyu hwesherufu

    Iyo kit inogona kuchengetwa kwemwedzi gumi nemaviri pane tembiricha yekamuri (15-25 ℃) kana 2-8 ℃ kwenguva yakareba (24 mwedzi).

    Buffer cRL1 inogona kuchengetwa pa4 ℃ kwemwedzi 1 mushure mekuwedzera 2-hydroxy-1-ethanethiol(kusarudza).


  • Zvakapfuura:
  • Zvinotevera:

  • RNA haina kubviswa kana RNA goho rakaderera

    Panowanzove nemhando dzakasiyana dzezvinhu zvinokanganisa kudzoreredza kushanda zvakanaka, senge: tishu sampuli RNA yemukati, nzira yekushanda, elution vhoriyamu, nezvimwe.

    1. Ice bath kana cryogenic (4 ° C) centrifugation yakaitwa panguva yekushanda.

    Kurudziro: Shanda pamhepo tembiricha (15-25 ° C) mukati mese muitiro, usaite ice yekugezesa uye centrifuge pane yakaderera tembiricha.

    2. Kuchengetedzwa kwemuenzaniso kusina kukodzera kana nguva yakawandisa yekuchengetedza sampuli.

    Kurudziro: Chengetedza samples pa -80 °C kana kuomesa munitrogen yemvura uye dzivirira kudzokororwa kushandiswa kwechando-nyoro;edza kushandisa matishu matsva kana maseru akagadzirwa eRNA kutorwa.

    3. Kusakwana sampuli lysis.

    Kurudziro: Paunenge uchigadzira matishu, ita shuwa kuti matishu akanyatsoenderana uye kuti masero ematishu akapatsanurwa zvakakwana kutsanangura kuburitswa kweRNA.

    4. Iyo enzi haina kuwedzerwa nemazvo.

    Kurudziro: Simbisa kuti RNase-Yemahara ddH2O inowedzerwa kudonha kusvika pakati peyekucheneswa column membrane.

    5. Vhoriyamu chaiyo yeabsolute ethanol haina kuwedzerwa kuBuffer RL2 kana Buffer RW2.

    Kurudziro: Tevedzera mirairo, wedzera iyo chaiyo vhoriyamu yeabsolute ethanol kuBuffer RL2 uye Buffer RW2 uye sanganisa zvakanaka usati washandisa kiti.

    6. Tissue sample dosage haina kukodzera.

    Kurudziro: Shandisa 10-20 mg yetishu kana (1-5) × 106masero pa 500 μl buffer RL1, sezvo kushandiswa kwematishu zvakanyanya kunogona kukonzera kuderedzwa kweRNA.

    7. Vhoriyamu yakashata isina kunaka kana kuti kuburitsa kusina kukwana.

    Kurudziro: Vhoriyamu yekujekesa yekoramu yekuchenesa ndeye 50-200 μl;kana iyo elution mhedzisiro isingagutsi, zvinokurudzirwa kuwedzera tembiricha yekuisa yekamuri nguva mushure mekuwedzera preheated RNase-Mahara ddH.2O, eg kwe5-10 min.

    8.Koramu yekuchenesa ine ethanol yakasara mushure meBuffer RW2 wash.

    Kurudziro: Kana paine ethanol yakasara mushure mekugezwa kweBuffer RW2, isina chinhu chubhu centrifugation ye1min, nguva yekushayikwa kwechubhu centrifugation oparesheni inogona kuwedzerwa kusvika 2min, kana koramu yekuchenesa inogona kuiswa pakamuri tembiricha ye5 min kuti ibvise zvakakwana zvakasara. ethanol.

    Yakanatswa RNA yakaderedzwa

    Hunhu hweRNA yakacheneswa hune hukama nezvinhu zvakaita sekuchengetedza sampuli, kusvibiswa kweRNase, uye kunyengera, nezvimwe.

    1. Tissue samples haisi kuchengetwa munguva.

    Kurudziro: Kana masamples ematishu kana maseru akasashandiswa nenguva mushure mekuunganidzwa, pakarepo cryopreserve pa -80 ° C kana liquid nitrogen.Kuti ubvise RNA, shandisa ichangobva kutorwa tishu kana sero sampuli pese pazvinogoneka.

    2. Kudzokororwa kwechando-kunyunguduka kwemasampuli ematishu.

    Kurudziro: Paunenge uchichengeta matishu samples, zviri nani kuacheka kuita zvidimbu zvidiki kuti zvichengetedzwe, uye kubvisa chimwe chezvidimbu paunenge uchizvishandisa kudzivirira kudzokororwa nechando-kunyunguduka kwesample uye kushatiswa kweRNA.

    3. RNase inounzwa kana kusapfeka magirovhosi anoraswa, masiki, nezvimwewo panguva yekushanda.

    Kurudziro: RNA yekuwedzera yekuedza inoitwa zvakanyanya mumakamuri akasiyana eRNA manipulation uye tafura inocheneswa isati yaedzwa.

    Pfeka magurovhosi anoraswa uye masks panguva yekuyedza kuderedza kuderedzwa kweRNA kunokonzerwa nekuunzwa kweRNase.

    4. Zvigadzirwa zvakasvibiswa neRNase panguva yekushandiswa.

    Kurudziro: Tsiva neMhuka Yakazara RNA Isolation Kit yezviyedzo zvinoenderana.

    5. Machubhu ecentrifuge, mazano, nezvimwewo zvinoshandiswa muRNA manipulation zvakasvibiswa neRNase.

    Kurudziro: Simbisa kuti machubhu ecentrifuge, matipi, mapipette, nezvimwewo anoshandiswa muRNA kudhirowa ese RNase-Mahara.

    Yakacheneswa yakawanikwa RNA inokanganisa kuyedza kwepasi

    RNA yakacheneswa nekoramu yekuchenesa, kana maion emunyu, mapuroteni akawandisa anozokanganisa kuyedza kwakadzika, senge: reverse transcription,Northern Blot et al.

    1. Iyo elutioned RNA ine salt ion residues.

    Kurudziro: Simbisa kuti vhoriyamu chaiyo ye ethanol yawedzerwa kuBuffer RW2 uye ita 2 mbiru yekunatsa washes pa centrifugal speed inoratidzwa kuti ishande;kana paine ion inosara yemunyu, siya mbiru yekuchenesa kuBuffer RW2 kwemaminetsi mashanu pane tembiricha yekamuri uye ita centrifugation kuti uwedzere kubviswa kwemunyu.

    2. Ethanol yakasara mune elutioned RNA.

    Recommendation: Simbisa kuti mushure mekugezesa buffer RW2, ita isina chinhu chubhu centrifugation operation pa centrifugation speed inoratidzwa kuti ishande, wedzera nguva ye empty tube centrifugation operation kusvika 2 min kana pachine ethanol residue, kana kuisiya patembiricha ye5. m

    Nyora meseji yako pano uye titumire kwatiri