Ropa SuperDirectᵀᴹ PCR Kit(UNG)-Heparin Ropa Yakananga PCR Master Mix yeGenotyping yeRopa
Kit Description:
Shandisa zvakananga heparin anticoagulated ropa rose se template yePCR amplification, ine high sensitivity uye yakakwirira amplification kunyatsoshanda.Iyo UNG anti-pollution system yakawedzerwa kudzivirira maitiro enhema.
◮Iyo sisitimu ine simba rekusimudzira uye inogona kunyatsoona genome uye yekunze inonangwa zvidimbu zveDNA muropa.
◮2 × SuperEasyTMMix (UNG) -Heparin Kushivirira kweropa: kusvika ku45% muropa revanhu uye 20% muropa rembeva.
◮Sample yakavharirwa zvizere, saka hapana chikonzero chekunetseka nezvekusvibiswa kwemuenzaniso uye nhema dzakanaka dzePCR.Anti-kusvibiswa PCR system
◮2 × SuperEasyTMSanganisa (UNG), nyatso bvisa kusvibiswa kunokonzerwa nePCR zvigadzirwa, uye simbisa iyo chaiyo uye nekururama kweamplification.
Zvinotsanangurwa
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMMix (UNG) -Heparin: Pahwaro hwe2 × SuperEasyTMMix-Heparin, dUTP inoshandiswa panzvimbo ye dTTP, uye iyo UNG enzyme inogona kuderedza dUTP template inowedzerwa panguva imwe chete, iyo inogona kunyatsodzivirira kusvibiswa kwePCR amplification products.Iine yakagadziridzwa D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer uye stabilizer, nezvimwewo. Mukuita kwePCR, ingo wedzera iro rakakodzera anticoagulated ropa rose, primers, uye ddH.2O kune inoenderana 2 × SuperEasyTM Mix (UNG) -Heparin kuti ishandiswe muPCR reaction.
6 × DNA Loading Buffer: Iyo Loading Buffer haina SDS.Zvinokurudzirwa kushandisa 6 × DNA Loading Buffer inosanganisirwa nekiti paunenge uchiita agarose gel electrophoresis kuitira kuti uwane yakanaka electrophoresis mhinduro.Usashandise Loading Buffer ine SDS, zvikasadaro pachava nemuswe wakakura wechiedza chakajeka munzira panguva ye electrophoresis, izvo zvinokanganisa mhedzisiro yekuedza.
Kit zvikamu
| 2 × SuperEasyTMMix(UNG)-Heparin |
| 6 × DNA Loading Buffer |
| Mirayiridzo |
Zvimiro&zvakanakira
-Iyo sisitimu ine simba rekusimudzira uye inogona kunyatsoona genome uye yekunze inonangwa zvidimbu zveDNA muropa.
-2 × SuperEasyTMMix (UNG) -Heparin Kushivirira kweropa: kusvika ku45% muropa revanhu uye 20% muropa rembeva.
-Sample yakavharirwa zvizere, saka hapana chikonzero chekunetseka nezvekusvibiswa kwemuenzaniso uye nhema dzakanaka dzePCR.Anti-kusvibiswa PCR system 2 × SuperEasyTMSanganisa (UNG), nyatso bvisa kusvibiswa kunokonzerwa nePCR zvigadzirwa, uye simbisa iyo chaiyo uye nekururama kweamplification.
Kit application
Yakatsaurirwa kunanga PCR kuzivikanwa kweHeparin anticoagulated ropa rose.(Kusanganisira: ropa remunhu, ropa rembeva, ropa rehuku, ropa reshiri, ropa remombe, ropa rembwa, nezvimwewo)
Workflow
Storage uye Sherufu upenyu
Iyo yese maitiro ekudzika-tembiricha echando bhokisi chekufambisa, kuve nechokwadi chekuti kit iri munzvimbo ye <4 ℃.
2 × SuperEasyTMMix (UNG) -Heparin, kana yakashandiswa kakawanda, inogonawo kuchengetwa pa4 ° C kuitira kuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi).
6 × DNA Loading Buffer, inogona kuchengetwa pa 4 ° C kana -20 ° C kwenguva yakareba.
