Cell Yakananga RT qPCR Kit

Cell Yakananga RT qPCR Kit

Tsananguro yeKit:

Iyi kit inoshandisa yakasarudzika lysis buffer system iyo inogona kukurumidza kuburitsa RNA kubva kune yakakwenenzverwa maseru sampuli yeRT-qPCR maitiro, nokudaro ichibvisa iyo inopedza nguva uye inorema RNA yekuchenesa maitiro. Iyo RNA template inogona kuwanikwa mumaminetsi manomwe chete. Iyo 5 × Direct RT Sanganisa uye 2 × Yakananga qPCR Sanganisa-SYBR reagents yakapihwa nekiti inogona kukurumidza uye kunyatso kuwana chaiyo-nguva yakawanda huwandu hwePCR mhedzisiro.

5 × Direct RT Sanganisa uye 2 × Yakananga qPCR Sanganisa-SYBR ine yakasimba inhibitor shiviriro, uye lysate yemasampuli inogona kushandiswa se template yeRT-qPCR zvakananga. Iyi kit ine yakasarudzika RNA yakakwira-kuyananisa Foregene reverse transcriptase, uye Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer uye stabilizer.


Chigadzirwa Chigadzirwa

Chigadzirwa Mateki

Magadzirirwo

200×20μl Rxns, 1000×20μl MaRxns

Kit zvikamu

Chikamu I

Buffer CL

Foregene Protease Kuwedzera II

Buffer ST

Chikamu II

DNA Eraser

5 × Direct RT Musanganiswa

2 × Yakananga qPCR Sanganisa-SYBR

50 × ROX Revhisi Dhayi

RNase-Yemahara ddH2O

Mirayiridzo

Zvimiro & zvakanakira

■  Zvakareruka uye zvinobudirira: neCell Direct RT tekinoroji, RNA sampuli dzinogona kuwanikwa mumaminetsi manomwe chete.

The Chidimbu chemuenzaniso chidiki, zvakadzika semasero gumi anogona kuyedzwa.

■ Kubudirira kwepamusoro: inogona kukurumidza kuona RNA mumasero akagadzirwa muna 384, 96, 24, 12, 6-tsime mahwendefa.

■ DNA Eraser inogona kukurumidza kubvisa magenomes akasunungurwa, inoderedza zvakanyanya kukanganisa kwezviitiko zvinotevera zvekuyedza.

■ Optimized RT uye qPCR system inoita kuti nhanho mbiri RT-PCR dzokerese kudhindisa kuve kwakanyanya uye PCR ive yakanyanya, uye inopokana neRT-qPCR reaction inhibitors.

Kit chishandiso

Makuriro ekushandisa: maseru akakwenenzverwa.

- RNA yakaburitswa nesampula lysis: inoshanda chete kune iyo RT-qPCR template yeichi kit.

- Iyo kit inogona kushandiswa kune zvinotevera zvinangwa: gene expression expression ongororo, kuongororwa kweRNA-mediated gene kunyararidza maitiro, kuongororwa kwezvinodhaka, nezvimwe.

Dhayagiramu

Cell Direct RT qPCR diagram

Kuchengetedza uye pasherufu hupenyu

Chikamu I cheichi kit chinofanira kuchengetwa pa4 ℃; Chikamu chechipiri chinofanirwa kuchengetwa pa -20 ℃.

 Foregene Protease Plus II inofanira kuchengetwa pa4℃, usagwamba pa -20 ℃.

 Reagent 2×Direct qPCR Sanganisa-SYBR inofanirwa kuchengetwa pa -20murima; kana ikashandiswa kazhinji, inogona zvakare kuchengetwa pa4Storage yekuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi).


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    Chigadzirwa Mapoka