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Cell Direct RT qPCR Kit—SYBR GREEN I Direct Cell Lysis Cell Ready Imwe-nhanho qRT-PCR Kits

Kit Description:

Cat.No.DRT-01011/01012

Yesero yakananga RT-qPCR uchishandisa gumi-1,000,000 masero,

uye kuita RT-qPCR zvakananga kubva kumaseru pasina kucheneswa kweRNA.

Masero anoiswa lysed zvakananga kuburitsa RNA yeRT-qPCR;iyo yakanyanya kushivirira sisitimu inoita kuti zvisave zvakakosha kuchenesa RNA uye zvakananga kushandisa sero lysates seRNA matemplate eRT maitiro.Fast uye nyore;kunzwisiswa kwepamusoro, kusimba kwakasimba, uye kugadzikana kwakanaka.

◮Ikareruka uye inoshanda: neCell Direct RT tekinoroji, masampuli eRNA anogona kuwanikwa mumaminetsi manomwe chete.

Iyo yemuenzaniso inodiwa idiki, yakaderera semaseru gumi anogona kuongororwa.

◮Kubuda kwepamusoro: inogona kukurumidza kuona RNA mumaseru akagadzirwa mu384, 96, 24, 12, 6-tsime mahwendefa.

DNA Eraser inogona kukurumidza kubvisa akaburitswa genomes, kuderedza zvakanyanya kukanganiswa pane inotevera yekuedza mhinduro.

Yakagadziridzwa RT uye qPCR sisitimu inoita kuti maviri-nhanho RT-PCR reverse transcript inyatsoshanda uye PCR yakanyatsojeka, uye inopokana neRT-qPCR reaction inhibitors.


Product Detail

Product Tags

FAQ

Dhaunirodha zviwanikwa

Tsanangudzo

Iyi kit inoshandisa yakasarudzika lysis buffer sisitimu inogona kukurumidza kuburitsa RNA kubva kune yakasimwa maseru masampula eRT-qPCR maitiro, nekudaro ichibvisa iyo inopedza nguva uye yakaoma RNA yekuchenesa maitiro.Iyo RNA template inogona kuwanikwa mumaminetsi manomwe chete.The 5×Yakananga RT Musanganiswa uye 2×Yakananga qPCR Musanganiswa-SYBR reagents inopihwa nekiti inogona nekukurumidza uye zvinobudirira kuwana chaiyo-nguva yakawanda yePCR mhinduro.

5×Yakananga RT Musanganiswa uye 2×Yakananga qPCR Mix-SYBR ine inhibitor kushivirira kwakasimba, uye lysate yemasampuli inogona kushandiswa setemplate yeRT-qPCR zvakananga.Iyi kit ine yakasarudzika RNA yakakwirira-yakabatana Foregene reverse transcriptase, uye Inopisa D-Taq DNA polymerase, dNTPs, MgCl.2, reaction buffer, PCR optimizer uye stabilizer.

Zvinotsanangurwa

200×20μl Rxns, 1000×20μl Rxns

Kit zvikamu

Chikamu I

Buffer CL

Foregene Protease Plus II

Buffer ST

Chikamu II

DNA Eraser

5 × Yakananga RT Musanganiswa

2 × Yakananga qPCR Musanganiswa-SYBR

50 × ROX Reference Dye

RNase-Yemahara ddH2O

Mirayiridzo

Zvimiro&zvakanakira

Yakareruka uye inoshanda: neCell Direct RT tekinoroji, RNA samples inogona kuwanikwa mumaminetsi manomwe chete.

∎ Sample kudiwa idiki, yakaderera semaseru gumi anogona kuongororwa.

■ High throughput: inogona kukurumidza kuona RNA mumaseru akagadzirwa mu384, 96, 24, 12, 6-tsime mahwendefa.

∎ DNA Eraser inogona kukurumidza kubvisa majenomes akaburitswa, kuderedza zvakanyanya kukanganiswa pamhedzisiro yekuedza.

■ Optimized RT uye qPCR sisitimu inoita kuti nhanho mbiri RT-PCR reverse transcription inyatsoshanda uye PCR yakanyatsojeka, uye inoshingirira kuRT-qPCR reaction inhibitors.

Kit application

Kuwanda kwekushandiswa: masero akagadzirwa.

- RNA yakaburitswa nesample lysis: inoshanda chete kune RT-qPCR template yeiyi kit.

- Iyo kit inogona kushandiswa kune zvinotevera zvinangwa: gene kutaura kuongororwa, kuoneswa kweSiRNA-mediated gene kunyaradza maitiro, kuongorora zvinodhaka, nezvimwe.

Mufananidzo

Cell Direct RT qPCR dhayagiramu

Storage uye Sherufu upenyu

Chikamu I chekiti ichi chinofanira kuchengetwa pa4 ℃;Chikamu II chinofanira kuchengetwa pa -20 ℃.

 Foregene Protease Plus II inofanira kuchengetwa pa4℃, usaome pa -20 ℃.

 Reagent 2×Yakananga qPCR Musanganiswa-SYBR inofanira kuchengetwa pa -20murima;kana ikashandiswa kazhinji, inogona zvakare kuchengetwa pa4℃ yekuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi).


  • Zvakapfuura:
  • Zvinotevera:

  • Real Time PCR primer dhizaini misimboti

    Pamberi Primer uye Reverse Primer

    Kune Real Time PCR, dhizaini yekutanga yakakosha.MaPrimers ane hukama nehunyanzvi uye kugona kwePCR amplification, uye inogona kugadzirwa ichitarisa kune anotevera misimboti:

    Kureba kwekutanga: 18-30bp.

    GC yemukati: 40-60%.

    Tm kukosha: Primer dhizaini software, senge Primer 5, inogona kupa iyo Tm kukosha kweiyo primer.Iyo Tm tsika dzekumusoro uye pasi pemvura dzekutanga dzinofanira kunge dziri padyo sezvinobvira.The Tm calculation formula inogonawo kushandiswa: Tm = 4 °C (G + C) + 2 °C (A + T).Paunenge uchiita PCR, tembiricha iri pazasi peprimer Tm kukosha kwe5 ° C inowanzo sarudzwa seye tembiricha yekudziya (iyo inowirirana kuwedzera kweiyo annealing tembiricha inogona kuwedzera iwo chaiwo maitiro ePCR).

    Primers uye PCR zvigadzirwa:

    Dhizaini primer PCR yekusimudzira chigadzirwa kureba ingangoita 100-150bp.

    Dhizaini yekutanga munzvimbo yechipiri yemaitiro eiyo template inofanira kudziviswa zvakanyanya sezvinobvira.

    Dzivisa kuumbwa kwe 2 kana anopfuura mabhesi anowirirana pakati pe 3′ migumo yekumusoro nekudzika kwemvura.

    Primer 3′ terminal base haigone kuvepo ne3 yekuwedzera yakatevedzana G kana C.

    Iwo maprimer pachawo haagone kuve neanopindirana zvimiro, zvikasadaro chimiro chebvudzi chinoumbwa, chinokanganisa PCR amplification.

    ATCG inofanirwa kugovaniswa zvakaenzana sezvinobvira mukutevedzana kwekutanga, uye 3 ′ terminal base inofanira kudzivirirwa seT.

    Appendikisi 1: Cell Direct RT-qPCR Kit chikamu chekuwedzera paki

    1.Sero Lysis Solution


    Sero Lysis Solution

    Kit zvikamu

    (24-tsime lysis system / tsime)

    DRT-01011-A1

    DRT-01011-A2

    100 T

    500 T

    ChikamuI

    Buffer CL

    20 ml

    100 ml

    Foregene Protease Plus II

    400 μl

    1 ml × 2

    Buffer ST

    1 ml × 2

    10 ml

    ChikamuII

    DNA Eraser

    400 μl

    1 ml × 2

    2.RT Mix


    RT Mix

    Kit zvikamu

    (20 μl reaction system)

    DRT-01011-B1

    200 T

    5 × Yakananga RT Musanganiswa

    800 μl

    RNase-Yemahara ddH2O

    1.7 ml × 2

     

    3.qPCR Mix


    qPCR Mix

    Kit zvikamu

    (20 μl reaction system)

    DRT-01011-C1

    DRT-01011-C2

    200 T

    1000 T

    2 × Yakananga qPCR Musanganiswa-SYBR

    1 ml × 2

    1.7 ml × 6

    50 × ROX Reference Dye

    40 μl

    200 μl

    RNase-Yemahara ddH2O

    1.7 ml

    10 ml

    Mirayiridzo yezvinyorwa:

    QuickEasyTM Cell Yakananga RT-qPCR Kit-SYBR Green I

    Nyora meseji yako pano uye titumire kwatiri