Mhuka Yese RNA Isolate Kit

Mhuka Yese RNA Isolate Kit

Tsananguro yeKit:

Kit Zvikamu:

YeBuffer RL1, YeBuffer RL2 

Buffer RW1, Buffer RW2

RNase-Yemahara ddH2O, DNA-Yekuchenesa Column

RNA-Chete Column

Magadzirirwo:

50 Preps, 200 Preps


Chigadzirwa Chigadzirwa

Chigadzirwa Mateki

Kits Rondedzero

Iyi kit inoshandisa spin mbichana uye fomula yakagadziriswa nekambani yedu, iyo inogona kubvisa yakachena-uye yepamusoro-soro yakazara RNA kubva kune dzakasiyana mhuka matishu ane hunyanzvi hwekuita.Inopa inoshanda DNA-yekuchenesa Column, iyo inogona nyore kupatsanura uye kushambadzira genomic DNA kubva kune supernatant uye tishu lysate, yakapusa uye yekuchengetedza-nguva; RNA-chete Column inogona kunyatso kusunga RNA uye inogona kugadziriswa pamwe chete neakasarudzika fomula Mazhinji esampuli.

Iyo sisitimu yese ndeyeRNase-Yemahara, kuitira kuti iyo yakaburitswa RNA isashoreke; Buffer RW1, Buffer RW2 buffer yekuwacha system, kuitira kuti iyo yakawanikwa RNA isina mapuroteni, DNA, ion, uye organic organic kusvibiswa.

Zvimiro & zvakanakira

■ Hapana chikonzero chekushushikana nezvekudzikiswa kweRNA; iyo sisitimu yese ndeye RNase-Yemahara
■ Bvisa zvinobudirira DNA-uchishandisa Column-yekuchenesa Column
■ Bvisa DNA pasina kuwedzera DNase
Simple Nyore-zvese mashandiro anopedzwa mukamuri tembiricha
■ Kukurumidza-kushanda kunogona kupedzwa mumaminitsi makumi matatu
■ Yakachengeteka-hapana organic reagent inodiwa
■ Kuchena kwepamusoro -OD260 / 280≈1.8-2.1

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Kit chishandiso

Iyo inokodzera kubviswa uye kunatswa kweiyo yakazara RNA kubva kune akasiyana siyana matsva kana echando matishu emhuka kana maseru akakwenenzverwa.

Chigadzirwa parameter

■ Maodzanyemba ekushandisa: yekutanga-tambo cDNA synthesis, RT-PCR, mamorekuru kuumbana, Northern Blot, nezvimwe.
■ Samples: nyama dzemhuka, maseru akura
■ Chiyero: Nyama 10-20mg, Masero (2-5) × 106
■ Maximum DNA inosunga kugona kwekuchenesa chikamu: 80 μg
■ Elution vhoriyamu: 50-200 μl

Kuyerera kwebasa

animal total RNA-simple workflow

Dhayagiramu

3 Animal Total RNA Isolation Kit7

Mhuka Yese RNA Isolate Kit yakarapwa 20mg
Nyowani mbeva sampuli, tora 5% yakanatswa yakazara RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: Itsvo
3: Chiropa 4: Mwoyo

Kuchengetedza uye pasherufu hupenyu

Iyo kit inokwanisa kuchengetwa kwemwedzi makumi maviri nemana muchitonho chemukati (15-25 ℃) kana 2-8 ℃ kwenguva yakareba. Buffer RL1 inogona kuchengetwa pa4 ℃ kwemwedzi mumwe mushure mekuwedzera β- mercaptoethanol (sarudzo).


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