RT Easy II (ine gDNase) Master Premix yekutanga-strand cDNA synthesis yeChaiyo Nguva PCR ine gDNase
Zvinotsanangurwa
25×20µl rxns,100×20µl rxns
RT NyoreTMII (Ne gDNase) ireverse transcription system yakanyatso kugadzirirwa Real Time PCR kuti ikurumidze kubvisa genomic DNA tsvina.5 × gDNase Mix inogona kukurumidza kubvisa genome yakasara muRNA pa42 ° C kwemaminetsi maviri, inonyatsodzivirira kupindira kwegenome pamigumisiro yeqPCR.
5 × RO-Zviri nyoreTMMusanganiswa une Foregene Reverse Transcriptase yakagadziridzwa neForegene, inova mhando nyowani yereverse transcriptase ine yakasimba RNA affinity.Iyo yakagadziridzwa sisitimu inoita kuti reverse transcription rate ikurumidze, uye yekutanga strand cDNA synthesis inogona kupedzwa pa42 ° C mumaminetsi gumi nemashanu.
Reverse transcription system yekiti iyi inogonawo kuchengetedza kuita kwakanaka pa50°C.Naizvozvo, inonyanya kubatsira kune yakaoma RNA matemplate uye inogona kunyora zviri nyore RNA matemplate ane yakakwira GC zvemukati uye yakaoma yechipiri chimiro.
Kit zvikamu
5 × gDNase Mix |
5 × RO-Zviri nyoreTMMix |
RNase-Yemahara ddH2O |
Mirayiridzo |
Zvimiro&zvakanakira
-Kukwanisa kukwanisa kubvisa gDNA, iyo inogona kubvisa gDNA mu template mukati memaminitsi maviri.
-Inoshanda reverse transcription system, zvinongotora maminetsi gumi nemashanu kupedzisa kuumbwa kweiyo yekutanga strand cDNA.
-Complex templates: matemplate ane yakakwira GC zvemukati uye yakaoma yechipiri chimiro anogona zvakare kudzoserwa nehunyanzvi hwepamusoro.
-High-sensitivity reverse transcription system, pg-level templates inogonawo kuwana yakakwirira-mhando cDNA.
-Iyo reverse transcription system ine yakanyanya kugadzikana yekupisa, iyo yakakwana yekuita tembiricha ndeye 42℃, uye ichine yakanaka reverse transcription performance pa50℃.
Kit application
Inoshandiswa zvakananga muChaiyo Nguva PCR kuongorora huwandu hwekutaura kwegene.
Inogona kukurumidza uye kunyatsoongorora kutsvaga RNA senge RNA hutachiona.
Reverse transcript yeRNA templates ine yakakwira GC zvemukati kana yakaoma yechipiri chimiro.
Workflow
Storage uye Sherufu upenyu
Iyo kit inofanira kuchengetwa pa -20°C.Chengetedza chigadzirwa mufiriji yekushisa nguva dzose pa -20 ° C pakarepo mushure mekugamuchira.Kana mamiriro ekuchengetedza akakodzera, chigadzirwa hachizodzikisi chero mashandiro panguva ye1-gore yechokwadi nguva.