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Direct PCR

Chii chinonzi Direct PCR

Direct PCR ndiyo nzira yeDNA amplification yakananga kubvaropa, mhuka mhuka,muswe wegonzo,sero,nzeve yemakonzo,mbizi hove, mazai ehove,dyara mashizha,mbeukana imwe yekutanga biological tissue sample pasina kuita DNA yekuzviparadzanisa nevamwe uye matanho ekuchenesa.Yakananga PCR tekinoroji inogona kuderedza zvakanyanya nguva yekuyedza, uye mutengo mu genotyping uye yakakwirira-huwandu mapurojekiti.Inopawo sarudzo iri nani kana watarisana nematambudziko ekusimudzira huwandu diki hwesample uko nhanho yekuchenesa inogona kutungamira mukurasikirwa kwemuenzaniso.

FOREGENE's PCR Mix ine simba rekuramba kushushikana, nekuda kwe FOREGENE simba rine patent yeTaq enzyme( mvumo yepamutemo: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)Yayo Taq enzyme inoshandisa DNA recombination tekinoroji kubatanidza iyo DNA inosunga chimiro dunhu rearchaeal nucleic acid inosunga mapuroteni neinodzivirira kupisa Taq DNA polymerase kuti ivake template DNA nekuwedzera hukama.Iyo yakavandudzwa inopisa-kutanga Taq inochengetedza mabasa akasiyana-siyana epakutanga DNA polymerase.Iyi Taq enzyme inogona kusunga ku template DNA uye kutanga DNA synthesis mu low concentration templates uye zvakaoma nharaunda.

For muenzaniso,itzviri nyore sekuwedzera yakocell,chirimwa (shizha diki, mudzi kana mhodzi) kana mhuka yemuenzaniso kunebuffer, uye nekuwedzera iyo yakasarudzikamaster mix withzvakanangamaprimers muPCR machubhu uye achimhanyisa kuburikidza neanopisa cycler.Kushanda kweKit kwakanakira chiyero chikuru chekuongorora uko kuchengetedza nguva kwakakosha zvakanyanya.

Iyo PCR reaction system ine UNG enzyme uye dUTP inosarudzwa zvichienderana nekuyedza zvinodiwa, iyo inogona kunyatso kudzivirira kusvibiswa kunokonzerwa nePCR amplification zvigadzirwa.

Zvakanakira uye Zvishandiso zveDirect PCR

mudiwa (5)
adada (1)
Zvakanakira Direct PCR
Application
Zvakanakira Direct PCR
    Direct PCR Traditional Method
(RNA/DNA Kucheneswa+Nenguva dzose PCR)
Kushandiswa Kwezvinhu Kushandiswa kweTissue Zvishoma Zvakawanda
Kugadzirira template Material Type Hapana kugaya kunodiwa Kukuya samples
Operation Imwe-chubhu mhando, 10min Kushanda kwakaoma, 1h
Flux 1-96 1-24
PCR Reaction Reaction System Yakagadzirirwa 2 × PCR musanganiswa dNTP,MgCl2,10×PCR Buffer,Taq Enzyme
Application

01 Kuzivikanwa kwepathogenic microorganisms

02 Genetically yakagadziridzwa chitupa, genotyping

03 Multiplex PCR / SNP kuongororwa / PCR-RFLP

04Sequencing/cloning

Foregene Direct PCR kits zvigadzirwa

Sero Yakananga RT-qPCR Kits
Ropa Rakananga PCR Kits
Mhuka Yakananga PCR Kits
Dyara Direct PCR Kits
IVD Kits
Sero Yakananga RT-qPCR Kits
Product Name Zvinotsanangurwa Catalog
Number
Storage Conditions
QuickEasyTMSero Yakananga RT-qPCR Kit-SYBR Green I/Taqman 200T DRT-01011
/DRT-01021
Chikamu I 4℃,Chikamu II -20℃
1000T DRT-01012
/DRT-01022
Ropa Rakananga PCR Kits
Product Name Zvinotsanangurwa Catalog
Number
Storage Conditions
Ropa SuperDirectTMPCR Kit (EDTA) 200 × 20μl rxns TP-04111 -20 ℃
2000 × 20μl rxns TP-04113
Ropa SuperDirectTMPCR Kit(EDTA) -UNG 200 × 20μl rxns TP-04121 -20 ℃
2000 × 20μl rxns TP-04123
Ropa SuperDirectTMPCR Kit (Heparin) 200 × 20μl rxns TP-04211 -20 ℃
2000 × 20μl rxns TP-04213
Ropa SuperDirectTMPCR Kit(Heparin) -UNG 200 × 20μl rxns TP-04221 -20 ℃
2000 × 20μl rxns TP-04223
Mhuka Yakananga PCR Kits
Product Name Zvinotsanangurwa Catalog
Number
Storage Conditions
Animal Tissue Direct PCR Kit 200 × 20μl rxns TP-01111 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-01113
Animal Tissue Yakananga PCR Kit(65℃ lysis) -UNG 200 × 20μl rxns TP-01121 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-01123
Mouse Muswe SuperDirectTM PCR Kit(yekamuri tembiricha lysis) 200 × 20μl rxns TP-01311 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-01313  
Mouse Muswe Wakananga PCR Kit
(65 ℃ lysis)
200 × 20μl rxns TP-01331 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-01333
Mouse Muswe Wakananga PCR Kit(65℃ lysis) -UNG 200 × 20μl rxns TP-01341 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-01343
Mbizi Hove Yakananga PCR Kit (65 ℃ lysis) 200 × 20μl rxns TP-01411 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-01413
Mbizi Hove Yakananga PCR Kit(65℃ lysis) -UNG 200 × 20μl rxns TP-01421 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-01423
Dyara Direct PCR Kits
Product Name Zvinotsanangurwa Catalog
Number
Storage Conditions
Dyara Leaf Yakananga PCR Kit 200 × 20μl rxns TP-02111 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-02113
Dyara Leaf Yakananga PCR Kit -UNG 200 × 20μl rxns TP-02121 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-02123
Dyara Leaf Yakananga PCR Plus Kit
(Polysaccharide polyphenol chirimwa)
200 × 20μl rxns TP-02131 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-02133
Plant Leaf Direct PCR Plus Kit -UNG (Polysaccharide polyphenol chirimwa) 200 × 20μl rxns TP-02141 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-02143
Dyara Mbeu Yakananga PCR Kit I 200 × 20μl rxns TP-03111 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-03113
Dyara Mbeu Yakananga PCR Kit I -UNG 200 × 20μl rxns TP-03121 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-03123
Dyara Mbeu Yakananga PCR Kit II 200 × 20μl rxns TP-03131 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-03133
Dyara Mbeu Yakananga PCR Kit II -UNG 200 × 20μl rxns TP-03141 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-03143
Dyara Mbeu Yakananga PCR Plus Kit I 200 × 20μl rxns TP-03151 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-03153
Dyara Mbeu Yakananga PCR Plus Kit I -UNG 200 × 20μl rxns TP-03161 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-03163
Dyara Mbeu Yakananga PCR Plus Kit II 200 × 20μl rxns TP-03171 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-03173
Dyara Mbeu Yakananga PCR Plus Kit II -UNG 200 × 20μl rxns TP-03181 Chikamu I 4℃,Chikamu II -20℃
2000 × 20μl rxns TP-03183

 

IVD Kits

*Covid Nucleic acid RT-PCR kit, yeORF1ab, N, E,S, uye mamwe anochinja, akadai seDelta musiyano, nezvimwe.

96Rxns/kiti

Benefits:

-Pedzisa bvunzo muinenge 1 awa

-Hapana kudikanwa kweRNA Kubvisa uye kuzviparadzanisa nevamwe

-Kunyanya kujeka uye kunzwa

Covid test kit kit tinya apa