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Direct PCR
Chii chinonzi Direct PCR
Direct PCR ndiyo nzira yeDNA amplification yakananga kubvaropa, mhuka mhuka,muswe wegonzo,sero,nzeve yemakonzo,mbizi hove, mazai ehove,dyara mashizha,mbeukana imwe yekutanga biological tissue sample pasina kuita DNA yekuzviparadzanisa nevamwe uye matanho ekuchenesa.Yakananga PCR tekinoroji inogona kuderedza zvakanyanya nguva yekuyedza, uye mutengo mu genotyping uye yakakwirira-huwandu mapurojekiti.Inopawo sarudzo iri nani kana watarisana nematambudziko ekusimudzira huwandu diki hwesample uko nhanho yekuchenesa inogona kutungamira mukurasikirwa kwemuenzaniso.
FOREGENE's PCR Mix ine simba rekuramba kushushikana, nekuda kwe FOREGENE simba rine patent yeTaq enzyme( mvumo yepamutemo: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)Yayo Taq enzyme inoshandisa DNA recombination tekinoroji kubatanidza iyo DNA inosunga chimiro dunhu rearchaeal nucleic acid inosunga mapuroteni neinodzivirira kupisa Taq DNA polymerase kuti ivake template DNA nekuwedzera hukama.Iyo yakavandudzwa inopisa-kutanga Taq inochengetedza mabasa akasiyana-siyana epakutanga DNA polymerase.Iyi Taq enzyme inogona kusunga ku template DNA uye kutanga DNA synthesis mu low concentration templates uye zvakaoma nharaunda.
For muenzaniso,itzviri nyore sekuwedzera yakocell,chirimwa (shizha diki, mudzi kana mhodzi) kana mhuka yemuenzaniso kunebuffer, uye nekuwedzera iyo yakasarudzikamaster mix withzvakanangamaprimers muPCR machubhu uye achimhanyisa kuburikidza neanopisa cycler.Kushanda kweKit kwakanakira chiyero chikuru chekuongorora uko kuchengetedza nguva kwakakosha zvakanyanya.
Iyo PCR reaction system ine UNG enzyme uye dUTP inosarudzwa zvinoenderana nezvinodiwa zvekuedza, izvo zvinogona kunyatso kudzivirira kusvibiswa kunokonzerwa nePCR amplification zvigadzirwa.
Zvakanakira uye Zvishandiso zveDirect PCR
Zvakanakira Direct PCR
Application
Zvakanakira Direct PCR
| Direct PCR | Traditional Method (RNA/DNA Kucheneswa+Nenguva dzose PCR) | ||
| Kushandiswa Kwezvinhu | Kushandiswa kweTissue | Zvishoma | Zvakawanda |
| Kugadzirira template | Material Type | Hapana kugaya kunodiwa | Kukuya samples |
| Operation | Imwe-chubhu mhando, 10min | Kushanda kwakaoma, 1h | |
| Flux | 1-96 | 1-24 | |
| PCR Reaction | Reaction System | Yakagadzirirwa 2 × PCR musanganiswa | dNTP,MgCl2,10×PCR Buffer,Taq Enzyme |
Application
01 Kuzivikanwa kwepathogenic microorganisms
02 Genetically yakagadziridzwa chitupa, genotyping
03 Multiplex PCR / SNP kuongororwa / PCR-RFLP
04Sequencing/cloning
Foregene Direct PCR kits zvigadzirwa
Sero Yakananga RT-qPCR Kits
Ropa Rakananga PCR Kits
Mhuka Yakananga PCR Kits
Dyara Direct PCR Kits
IVD Kits
Sero Yakananga RT-qPCR Kits
| Product Name | Zvinotsanangurwa | Catalog Number | Storage Conditions |
| QuickEasyTMSero Yakananga RT-qPCR Kit-SYBR Green I/Taqman | 200T | DRT-01011 /DRT-01021 | Chikamu I 4℃，Chikamu II -20℃ |
| 1000T | DRT-01012 /DRT-01022 |
Ropa Rakananga PCR Kits
| Product Name | Zvinotsanangurwa | Catalog Number | Storage Conditions |
| Ropa SuperDirectTMPCR Kit (EDTA) | 200 × 20μl rxns | TP-04111 | -20 ℃ |
| 2000 × 20μl rxns | TP-04113 | ||
| Ropa SuperDirectTMPCR Kit(EDTA) -UNG | 200 × 20μl rxns | TP-04121 | -20 ℃ |
| 2000 × 20μl rxns | TP-04123 | ||
| Ropa SuperDirectTMPCR Kit (Heparin) | 200 × 20μl rxns | TP-04211 | -20 ℃ |
| 2000 × 20μl rxns | TP-04213 | ||
| Ropa SuperDirectTMPCR Kit(Heparin) -UNG | 200 × 20μl rxns | TP-04221 | -20 ℃ |
| 2000 × 20μl rxns | TP-04223 |
Mhuka Yakananga PCR Kits
| Product Name | Zvinotsanangurwa | Catalog Number | Storage Conditions |
| Animal Tissue Direct PCR Kit | 200 × 20μl rxns | TP-01111 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-01113 | ||
| Animal Tissue Yakananga PCR Kit(65℃ lysis) -UNG | 200 × 20μl rxns | TP-01121 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-01123 | ||
| Mouse Muswe SuperDirectTM PCR Kit(yekamuri tembiricha lysis) | 200 × 20μl rxns | TP-01311 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-01313 | ||
| Mouse Muswe Wakananga PCR Kit (65 ℃ lysis) | 200 × 20μl rxns | TP-01331 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-01333 | ||
| Mouse Muswe Wakananga PCR Kit(65℃ lysis) -UNG | 200 × 20μl rxns | TP-01341 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-01343 | ||
| Mbizi Hove Yakananga PCR Kit (65 ℃ lysis) | 200 × 20μl rxns | TP-01411 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-01413 | ||
| Mbizi Hove Yakananga PCR Kit(65℃ lysis) -UNG | 200 × 20μl rxns | TP-01421 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-01423 |
Dyara Direct PCR Kits
| Product Name | Zvinotsanangurwa | Catalog Number | Storage Conditions |
| Dyara Leaf Yakananga PCR Kit | 200 × 20μl rxns | TP-02111 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-02113 | ||
| Dyara Leaf Yakananga PCR Kit -UNG | 200 × 20μl rxns | TP-02121 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-02123 | ||
| Dyara Leaf Yakananga PCR Plus Kit (Polysaccharide polyphenol chirimwa) | 200 × 20μl rxns | TP-02131 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-02133 | ||
| Plant Leaf Direct PCR Plus Kit -UNG (Polysaccharide polyphenol chirimwa) | 200 × 20μl rxns | TP-02141 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-02143 | ||
| Dyara Mbeu Yakananga PCR Kit I | 200 × 20μl rxns | TP-03111 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-03113 | ||
| Dyara Mbeu Yakananga PCR Kit I -UNG | 200 × 20μl rxns | TP-03121 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-03123 | ||
| Dyara Mbeu Yakananga PCR Kit II | 200 × 20μl rxns | TP-03131 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-03133 | ||
| Dyara Mbeu Yakananga PCR Kit II -UNG | 200 × 20μl rxns | TP-03141 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-03143 | ||
| Dyara Mbeu Yakananga PCR Plus Kit I | 200 × 20μl rxns | TP-03151 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-03153 | ||
| Dyara Mbeu Yakananga PCR Plus Kit I -UNG | 200 × 20μl rxns | TP-03161 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-03163 | ||
| Dyara Mbeu Yakananga PCR Plus Kit II | 200 × 20μl rxns | TP-03171 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-03173 | ||
| Dyara Mbeu Yakananga PCR Plus Kit II -UNG | 200 × 20μl rxns | TP-03181 | Chikamu I 4℃，Chikamu II -20℃ |
| 2000 × 20μl rxns | TP-03183 |
IVD Kits
*Covid Nucleic acid RT-PCR kit, yeORF1ab, N, E,S, uye mamwe anochinja, akadai seDelta musiyano, nezvimwe.
96Rxns/kiti
Benefits:
-Pedzisa bvunzo muinenge 1 awa
-Hapana kudikanwa kweRNA Kubvisa uye kuzviparadzanisa nevamwe
-Kunyanya kujeka uye kunzwa
