Chii chinonzi DirectPCR?
DirectPCR tekinoroji inoreva kuti pasina kuparadzanisa uye kuchenesa nucleic acid mamorekuru (kusanganisira DNA neRNA) yemhando dzakasiyana siyana dzetishu, chete tishu uye sero chimiro chinoparadzwa ne lysis ye protease, iyo nucleic acid inoburitswa mu lysis solution, uye mhinduro yelysis inowedzerwa zvakananga.Iyo PCR reaction system ndeye tekinoroji yekusimudzira yejini rinotangwa.
Kutsvaga shanduko
Kuparadzaniswa uye kubviswa kweiyo nucleic acids nyaya
Kubva pakugadzirwa kwePCR tekinoroji makore makumi matatu apfuura, vaongorori vanga vachinetseka nekuparadzaniswa uye kubviswa kweiyo nucleic acids.Kushandisa matishu samples kuita zvakananga PCR maitiro ndiko kurota kwevazhinji vaongorori.Asi hope idzi hadzina kuzadziswa kwemakore makumi matatu.Chikonzero ndechekuti iyo disintegrated tishu inoburitsa yakawanda inhibitory ingredients.
Izvi zvipingamupinyi zvikamu zvichava nechisimba chakasimba chekudzivirira paPCR maitiro, zvimwe zvichaita kuti primer uye template isakwanise kusunga, vamwe vane simba reprotein denaturation basa, zvichiita kuti nucleic acid polymerase isashande, uye zvimwe zvinodzivirira zvakananga primer uye template kubva pakusunga .Izvi zvese zvinhu zvinoita kuti PCR iite kuti isafambe zvakanaka.
Sechinyakare, vanhu vanopota vachishandisa PCR ekuwedzera kuwedzera kunzwisiswa, kujeka uye kukwidziridza kunyatsoita kwePCR maitiro.Zvisinei, nekuda kwePCR inhibitors yakasiyana-siyana iri mu nucleic acid templates kubva kune zvakasiyana-siyana, kushandiswa kwenguva refu kwenhamba yakawanda yekusimudzira zviri pachena kuti haina kukodzera kurasikirwa, mutengo wakakwirira, uye kushanda kwakaoma.
Foregene DirectPCR
Kubudirira kukuru pasirese --Two Techniques
Foregene akaita budiriro mundima yetekinoroji iyi pasi rose.Foregene DirectPCR tekinoroji ine maviri ehunyanzvi mapoinzi, ayo anogonesa Foregene kuve piyona uye mutungamiri weDirectPCR tekinoroji.
Kutanga, iyo patented nucleic acid polymerase modification nzira.Forgene ine patented nuclease modification method, iyo yakanangwa panzvimbo inosunga kuita kuti nuclease uye template ive yakasimba, ine chiitiko chepamusoro, kukwirira kwekusimudzira uye kugadzikana kwekupisa.
Chechipiri, iyo PCR Musanganiswa fomula yakagadziridzwa yemhando dzakasiyana, yakasarudzika maitiro ekusimudzira, optimizers uye stabilizers, inosimudzira zvikuru kuramba kwepolymerase kuPCR inhibitors uye kuve nechokwadi chekusimudzira.
Izvo chaizvo nekuda kwezvataurwa pamusoro apa maviri akakosha tekinoroji mabudiro akaita Foregene akaziva iyo chaiyo DirectPCR pasi rose.Zvingave zvakajairika matishu emhuka, zvinwiwa zvemuviri, matishu ekudyara, mashizha kana midzi matipi, kana kunyange mbeu yekudyara, vashandisi vanogona kuwana yakananga PCR amplification pasina chero mechaniki kuputsika kana inonetesa nucleic acid yekuchenesa maitiro.
Kune iyo DirectPCR kits yemiti yakatevedzana uye mhuka dzakateedzana dzakatangwa, Foregene inogona kudada kutaura kuti isu tine inotungamira mashandiro epasirese zviratidzo.Mune ramangwana, Foregene icharamba ichivhura nhevedzano yezvigadzirwa zvichine zvinotungamira pasi rose zviratidziro (kana zvakatosiyana).
Kuti uwane rumwe ruzivo, pinda:
http://www.foregene.com/http://www.foreivd.com/
Nguva yekutumira: Feb-27-2017
