Dyara shizha Direct PCR Plus kit (isina Sampling Zvishandiso) Protocol Direct PCR kubva kuPlat Material.
Tsanangudzo
Ichi chigadzirwa chinoshandisa yakasarudzika lysis buffer sisitimu kumisa chirimwa mashizha.Iyo lysate inogona kushandiswa se template pasina kucheneswa.Mushure mekuwedzera maprimers, iyo PCR Musanganiswa wekiti iyi inogona kushandiswa kukwidziridza.
Zvinotsanangurwa
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Kit zvikamu
Chikamu I | Buffer PS1 |
Buffer P2 | |
6×DNA Loading Buffer | |
Chikamu II | Buffer PS2 |
2× Leaf PCR NyoreTMMix |
Zvimiro&zvakanakira
■ Hapana kunotora nguva uye kunodhura kuchenesa DNA
■ Zvinyorwa zvishoma
■ Nyore-sample inogona kuiswa pasi pePCR reaction kana lysis reaction pasina kucheka kana kugaya
■ 2 × Sanganisa, kuderedza sampuli kurodha zvikanganiso uye reaction system yekugadzirira nguva
■ Kugadzirira kweFast-template kunogona kupedzwa mumaminitsi gumi, kuita kwePCR kunogona kupera mukati memaminitsi makumi mashanu.
■ High throughput-lysis reaction inogona kupedzwa mu96-tsime PCR ndiro
Kit parameters
Kushandiswa: Kuzivikanwa kwezvirimwa zve transgenic, genotyping, nezvimwe.
Sample: chirimwa mashizha akapfuma mu polysaccharide kana polyphenol
Saizi yemuenzaniso: Diameter 2-3mm (yakananga nzira), dhayamita 5-7mm (lysis nzira)
Kuonekwa kwemhando: PCR chigadzirwa ≤1kb