RT Easyᵀᴹ II Master Premix yekutanga-strand cDNA synthesis yeChaiyo Nguva PCR
Kit Description:
-Inoshanda reverse transcription system, zvinongotora maminetsi makumi maviri kupedzisa kusanganiswa kweiyo yekutanga strand cDNA.
-Yakakwirira-sensitivity reverse transcription system, pg-level matemplate anogonawo kuwana yakakwirira-mhando cDNA.
-Iyo reverse transcription system ine yakanyanya kugadzikana yekupisa, tembiricha yemaitiro inogona kunge yakakwira kusvika 50 ℃, uye ine yakanaka reverse transcription performance.
-Kunyangwe masampuli eRNA asina kuchena (doro rinosvika 60%, guanidine munyu unosvika 750mM) inogona kuisirwa reverse transcription reaction.
- 2 × RT OR-Zviri nyoreTM Musanganiswa wakawedzera optimized reverse transcription primers (Random Primer, Oligo(dT)18 Primer).
Kit Descriptions
Foregene's 2 × RT OR-Zviri nyoreTMMusanganiswa unogona kubatanidza iyo yekutanga-strand cDNA patembiricha yakakwira kusvika 50 ° C, iyo inobatsira kune reverse transcription reaction yeyakaoma yechipiri chimiro RNA matemplate.
Iyo RNA yakacheneswa murabhoritari kazhinji ine doro uye guanidine munyu zvisaririra, izvo zvine simba inhibitory properties pane akawanda reverse transcriptases, zvichikonzera zvisingagutsi reverse transcription effects kana yakaderera reverse transcription performance.Foregene 2 × RT Musanganiswa inoratidza kushivirira kwakanyanya kudoro uye guanidine munyu.Iyo yakanyanya kushivirira kudoro muRNA samples ndeye 60%, uye yakanyanya kushivirira kune guanidine munyu ndeye 750mM.Kunyangwe isina kuchena RNA inogona kushandiswa kudzosera reverse transcription reaction neForegene 2 × RT OR-Easy.TMMix.
2 × RT OR-Zviri nyoreTMMusanganiswa ndeye inokurumidza reverse transcription reagent yakanyatso kugadzirirwa Real Time PCR.Iyi sisitimu ine yakakwira reverse transcription performance uye haidi kuwedzera chero maprimers, uye inogona kuita zvakanaka reverse transcription reaction padiki diki rematemplate eRNA.Iyo yakasarudzika reaction system inoita kuti maitiro eRT ave nyore, nekukurumidza uye zvakanyanya kushanda.Yekutanga tambo cDNA synthesis inogona kupedzwa mumaminitsi makumi maviri.Ichi chigadzirwa chinogona kushandiswa pamwe chete neForegene's fluorescent quantitative chigadzirwa Chaiyo Nguva PCR Nyore.TMkuti uwane mhinduro dzemhando yepamusoro dzekuyedza.
Zvinotsanangurwa
| RT NyoreTMII Master Premix yekutanga-strand cDNA synthesis yeChaiyo Nguva PCR | |||
| Kit zvikamu | RT-01021 | RT-01022 | RT-01023 |
| 50 T (10μl system) | 200 T(10μl system) | 800 T (10μl system) | |
| 2 × RT OR-Zviri nyoreTMMix | 0.25ml | 1ml | 1ml × 4 |
| RNase-Yemahara ddH2O | 1.7ml | 1.7ml | 1.7ml × 3 |
| Bhuku reMirayiridzo | 1 chidimbu | 1 chidimbu | 1 chidimbu |
Zvimiro&zvakanakira
-Inoshanda reverse transcription system, zvinongotora maminetsi makumi maviri kupedzisa kusanganiswa kweiyo yekutanga strand cDNA.
-Yakakwirira-sensitivity reverse transcription system, pg-level matemplate anogonawo kuwana yakakwirira-mhando cDNA.
-Iyo reverse transcription system ine yakanyanya kugadzikana yekupisa, tembiricha yemaitiro inogona kunge yakakwira kusvika 50 ℃, uye ine yakanaka reverse transcription performance.
-Kunyangwe masampuli eRNA asina kuchena (doro rinosvika 60%, guanidine munyu unosvika 750mM) inogona kuisirwa reverse transcription reaction.
- 2 × RT OR-Zviri nyoreTMMusanganiswa wakawedzera optimized reverse transcription primers (Random Primer, Oligo(dT)18Primer).
Kit application
-Inoshandiswa yakananga muChaiyo Nguva PCR kuongorora huwandu hwekutaura kwegene
-Inokwanisa kukurumidza uye kunyatsoongorora zvidiki zveRNA senge RNA mavhairasi
- Reverse kunyorwa kweRNA matemplate ane yakakwira GC zvemukati kana yakaoma yechipiri chimiro
Kuyerera kwebasa
Kuchengetedza uye hupenyu hwesherufu
1. Mamiriro ekufambisa
Iyo yese maitiro ekudzika-tembiricha yekutakura ice box, kuve nechokwadi chekuti kit iri mumamiriro e <4°C.
2. Mamiriro ekuchengetedza
RT NyoreTMII inochengetwa pa -20 ° C.Chengetedza chigadzirwa mufiriji yekushisa nguva dzose pa -20 ° C pakarepo mushure mekugamuchira.Kana mamiriro ekuchengetedza akakodzera, chigadzirwa hachizodzikisi chero mashandiro panguva ye1-gore yechokwadi nguva.
