Viral RNA Isolation Kit yeViral RNA Kucheneswa kubva kuPlasma, Serum, uye Mamwe Masampuli
Kit Description:
Cat.No.RE-02011/02014
Kucheneswa kwehutachiona RNA kubva kuplasma, serum, sero-isina zvinwiwa zvemuviri, sero-tsika tsika supernatants.
Kurumidza kuparadzanisa uye kuchenesa hutachiona RNA kubva kumasampuli akadai seplasma, serum, sero-isina zvinwiwa zvemuviri uye sero tsika supernatants.
-Hapana chikonzero chekunetseka nezveRNA kuderedzwa.Iyo yese kit ndeye RNase-Yemahara
-Zviri nyore-mabasa ese anopedzwa patembiricha yekamuri
-Kukurumidza-kushanda kunogona kupedzwa mumaminitsi makumi maviri
-Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatso chenesa RNA
-Yakachengeteka-hapana organic reagent inoshandiswa
-Kukura sampuli yekugadzirisa simba-kusvika ku200μl samples inogona kugadziriswa nguva imwe neimwe.
-Hunhu hwepamusoro-iyo RNA yakacheneswa yakanyanya kuchena, isina mapuroteni uye kumwe kusvibiswa, uye inogona kusangana neyakasiyana-siyana yekudzika yekuyedza maapplication.
Tsanangudzo
Iyo kit inoshandisa spin column uye fomula yakagadziridzwa neForegene, iyo inogona kunyatso kuburitsa yakakwira-kuchena uye yemhando yepamusoro hutachiona RNA kubva kumasampuli akadai seplasma, serum, sero-isina masero emuviri, uye sero tsika supernatant.Iyo kit inonyanya kuwedzera Linear Acrylamide, iyo inogona kutora nyore zvidiki zveRNA kubva kumasampuli.RNA-Chete Column inogona kusunga RNA zvine mutsindo.Iyo kit inogona kugadzirisa nhamba huru yemasampuli panguva imwe chete.
Iyo yese kit haina RNase, saka iyo yakacheneswa RNA haizodzikisirwe.Buffer viRW1 uye Buffer viRW2 inogona kuve nechokwadi chekuti yakawanikwa hutachiona nucleic acid isina mapuroteni, nuclease kana kumwe kusvibiswa, iyo inogona kushandiswa zvakananga kuzasi kwema molecular biology kuyedza.
Zvinotsanangurwa
50 Preps, 200 Preps
Kit zvikamu
| Linear Acrylamide |
| Buffer viRL |
| Buffer viRW1, Buffer viRW2 |
| RNase-Yemahara ddH2O |
| RNA-Chete Column |
| Mirayiridzo |
Zvimiro&zvakanakira
-Hapana chikonzero chekunetseka nezveRNA kuderedzwa.Iyo yese kit ndeye RNase-Yemahara
-Zviri nyore-mabasa ese anopedzwa patembiricha yekamuri
-Kukurumidza-kushanda kunogona kupedzwa mumaminitsi makumi maviri
-Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatso chenesa RNA
-Yakachengeteka-hapana organic reagent inoshandiswa
-Kukura sampuli yekugadzirisa simba-kusvika ku200μl samples inogona kugadziriswa nguva imwe neimwe.
-Hunhu hwepamusoro-iyo RNA yakacheneswa yakanyanya kuchena, isina mapuroteni uye kumwe kusvibiswa, uye inogona kusangana neyakasiyana-siyana yekudzika yekuyedza maapplication.
Kit parameters
Kit application:
Inokodzera kubviswa uye kucheneswa kwehutachiona RNA mumasampuli akadai seplasma, serum, sero-isina masero emvura uye sero tsika supernatant.
Kuyerera kwebasa
Kuchengetedza mamiriro
- Iyo kit inogona kuchengetwa kwemwedzi makumi maviri nemana pakamuri tembiricha (15-25 ℃), kana 2-8 ℃ kwenguva yakareba.
-Linear Acrylamide mhinduro inogona kuchengetwa pakamuri tembiricha kwemazuva manomwe.Mushure mekugamuchira kit, ndapota tora Linear Acrylamide mhinduro kunze uye uichengete pa -20 ℃.
-Mushure mekuwedzera Linear Acrylamide kuBuffer viRL, inogona kuchengetwa pa2-8 ℃ kusvika ku48h.Ndapota shandisa mhinduro itsva yakagadzirwa.
Nhungamiro dzekuongorora matambudziko
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
Hapana RNA inogona kutorwa kana goho re nucleic acid rakaderera
Pane kazhinji zvinhu zvakawanda zvinokanganisa kudzoreredza kushanda zvakanaka, senge: sampuli RNA yemukati, nzira yekushanda, elution vhoriyamu, nezvimwe..
Kuongorora kwezvikonzero zvinowanzoitika:
1.Ice kugeza kana kuderera-kupisa (4 ° C) centrifugation panguva yekushanda.
Zano: Tembiricha yekamuri (15-25 ° C) mashandiro, usambofa kugeza nechando uye yakaderera tembiricha centrifuge.
2. Zvisizvo sampuli yekuchengetedza kana sampuli yekuchengetedza kwenguva yakareba.
Zano: Chengetedza sampuli pa -80 ° C kana kuomesa munitrogen yemvura, uye dzivirira kudzokororwa kushandiswa kwechando-nyoro;edza kushandisa masampuli achangobva kuunganidzwa eRNA kutorwa.
3.Isina kukwana sampuli lysis
Kurudziro: Ndokumbira utarise kuti sampuli uye mhinduro yekushanda (Linear Acrylamide) yakanyatso kusanganiswa uye yakanamirwa kwemaminetsi gumi patembiricha yekamuri (15-25 ° C)
4.The eluent yakawedzerwa zvisizvo
Kurudziro: Ita shuwa kuti RNase-Yemahara ddH2O inowedzerwa pakati pe membrane yekoramu yekuchenesa.
5.Improper volume yeanhydrous ethanol muBuffer viRW2
Zano: Ndokumbira uteedzere mirairo, wedzera iyo chaiyo vhoriyamu yeanhydrous ethanol kuBuffer viRW2 wosanganisa zvakanaka usati washandisa kiti.
6.Improper sampuli kushandiswa.
Zano: 200µl yesampuli pa500μl yeBuffer viRL.Yakawandisa sampuli vhoriyamu inokonzeresa kuderedzwa kweRNA yekubvisa mwero.
7.Improper elution vhoriyamu kana kusakwana elution.
Zano: Huwandu hwakajeka hwekoramu yekuchenesa ndeye 30-50μl;kana iyo elution effect isingagutsi, zvinokurudzirwa kuwedzera pre-heated RNase-Mahara ddH.2O uye wedzera nguva yekuisa pane tembiricha yekamuri, senge 5-10min
8.Kunatsa koramu ine ethanol yasara mushure mekugezeswa muBuffer viRW2.
Zano: Kana ethanol ichiri kusara mushure mekugezeswa muBuffer viRW2 uye isina chinhu-chubhu centrifugation kwe2min, mbiru yekunatsa inogona kusiiwa patembiricha yekamuri kwemaminetsi mashanu mushure mekunge isina chinhu-chubhu centrifugation kubvisa zvachose ethanol yasara.
Kushatiswa kwemamorekuru eRNA akacheneswa
Hunhu hweRNA yakacheneswa hunoenderana nezvinhu zvakaita seyekuchengetedza sampuli, kusvibiswa kweRNase, uye kushanda.
Kuongorora kwezvikonzero zvinowanzoitika:
1.Masampuli akaunganidzwa haana kuchengetwa munguva.
Zano: Kana sampu ikasashandiswa nenguva mushure mekuunganidzwa, ndapota ichengetedze pa -80 ℃ kana mvura yenitrogen nekukasira.Pakubviswa kwemamorekuru eRNA, edza kushandisa masampuli achangobva kuunganidzwa pese pazvinogoneka.
2.Kuunganidza samples kwaive kutonhora uye kunyunguduka kakawanda.
Zano: Dzivisa kudzokorora kutonhora nekunyunguduka (kwete kanopfuura kamwe) panguva yekuunganidza nekuchengetedza sampuli, zvikasadaro goho re nucleic acid richadzikira.
3.RNase yakaunzwa muimba yekushanda kana pasina magurovhosi anoraswa, masiki, nezvimwe zvakapfekwa.
Zano: Kubviswa kweRNA mamorekuru kuyedza kunoitwa zvakanyanya mune yakaparadzana RNA kamuri yekuvhiya, uye tafura yekuedzwa inocheneswa isati yaedzwa.Pfeka magurovhosi anoraswa uye masiki panguva yekuyedza kudzivirira kusvibiswa kweRNA kunokonzerwa neRNase sumo.
4.Iyo reagent yakasvibiswa neRNase panguva yekushandiswa.
Zano: Tsiva neViral RNA Isolation Kit itsva yezviedzo zvinoenderana.
5.Kusvibiswa kweRNase kwemachubhu ecentrifuge, matipi epipette, etc. Zano: Iva nechokwadi chekuti machubhu ecentrifuge, matipi epipette, uye mapipette ese ari RNase-Mahara.
Iwo akacheneswa mamorekuru eRNA akakanganisa kuyedza kuzasi
Mamorekuru eRNA akacheneswa nekoramu yekuchenesa achakanganisa kuyedza kuzasi kana paine maion emunyu akawandisa kana mapuroteni, akadai se: reverse transcription, Northern Blot, nezvimwewo.
1.Pane asara ayoni emunyu mune eluted RNA mamorekuru.
Kurudziro: Ita shuwa kuti iyo chaiyo vhoriyamu yeanhydrous ethanol yawedzerwa kuBuffer viRW2, uye geza koramu yekuchenesa kaviri zvinoenderana neiyo chaiyo centrifugation kumhanya pamirayiridzo yekushanda; Kana pachine maion emunyu asara, unogona kuwedzera Buffer viRW2 kukoromo yekuchenesa, uye woisiya pakamuri tembiricha ye5min.Zvadaro ita centrifugation kubvisa salt ions kusvibiswa kusvika pamwero mukuru
2.Pane yasara ethanol mu eluted RNA mamorekuru
Zano: kana uchinge wasimbisa kuti mbiru dzekuchenesa dzakasukwa neBuffer viRW2, ita isina chinhu-chubhu centrifugation zvinoenderana nekumhanya kwecentrifugal pamirayiridzo yekushandisa.Kana kuchine ethanol yasara, inogona kusiiwa kwemaminetsi mashanu pakudziya kwekamuri mushure meisina-tube centrifugation kubvisa yasara ethanol kusvika pamwero mukuru.
Mirayiridzo yezvinyorwa:
Viral RNA Isolation Kit Instruction Manual
