Mouse Muswe DNA Mini Kit Genomic DNA Extraction Kits kubva Mouse Muswe
Kit Description:
Iyo inomhanyisa pasi rose kit yekubvisa mbeva muswe genomic DNA, iyo inogona kubvisa yemhando yepamusoro genomic DNA kubva pamuswe wembeva mukati meawa imwe.
◮Hapana RNase kusvibiswa:DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva mujenomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
◮KumhanyaForegene Protease ine chiitiko chepamusoro kupfuura maprotease akafanana, uye inogaya masampula enyama nekukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me20-80 maminetsi.
◮Yakanakira:Iyo centrifugation inoitwa pakamuri tembiricha, uye hapana chikonzero che4 ° C yakaderera-tembiricha centrifugation kana ethanol kunaya kweDNA.
◮Safety:Hapana organic reagent extract inodiwa.
◮Hunhu hwepamusoro:Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
◮Micro-elution system:Inogona kuwedzera kuwanda kweiyo genomic DNA, iyo yakanakira kudzika kwerwizi kana kuyedza.
Tsanangudzo
Pamusana pekugadzirwa uye maitiro emuswe wembeva pachayo, kubviswa kwembeva muswe genomic DNA yakagara ichitambudza vatsvakurudzi: imwe ndeyokuti nguva yekugadzirisa yemuswe wemouse yakareba uye genomic DNA inoderedzwa nyore nyore, imwe yakaoma kubata uye kuchena kweiyo yakabudiswa genomic DNA yakaderera.
Kiti iyi inoshandisa DNA-Chete Column inogona kunyatso kusunga DNA, nyowani-itsva Foregene Protease uye yakasarudzika buffer sisitimu, inogona kugaya mbeva yemuswe samples mukati me45 maminetsi, nekudaro ichideredza kuderedzwa kwegenomic DNA uye kupfupisa sampuli yekugadzirisa nguva.Iyo kit inogona kubvisa 10-20μg yemhando yepamusoro, yakakwirira-kuchena genomic DNA kubva ku0.5-1cm pwere kana yakakura mbeva miswe mukati me60-90min.
Iyo DNA-chete silica gel matrix zvinhu inoshandiswa mu spin column chinhu chitsva chakasarudzika chekambani, chinogona kunyatso uye kunyanya adsorb DNA.Iyo yakanyanya adsorption kugona kweDNA i80μg.Iyo yakasarudzika buffer uye elution system inogona kukwirisa kubviswa kweRNA, Kusachena mapuroteni, ion uye mamwe organic komisheni muchitokisi.Iyo yakabviswa genomic DNA fragment yakakura, yakakwirira mukuchena, yakagadzikana uye yakavimbika mumhando, uye saizi yeDNA fragment yakagadzikana panenge 23kb.
Iyo kititi inogonawo kushandiswa kubvisa mamwe matukisi embeva kunze kwemuswe wembeva, kusanganisira nzeve dzembeva, mbeva tsandanyama, chiropa chembeva uye mamwe matissue, kuitira kuti kit imwe inogona kushandiswa nekuda kwezvikonzero zvakawanda uye kusangana nezvinodiwa zvevashandisi kune dzakasiyana ongororo.
Zvinotsanangurwa
50 Preps, 100 Preps, 250 Preps
Kit zvikamu
| Buffer TL1 |
| Buffer TL2* |
| Buffer PW* |
| Buffer WB |
| Buffer EB |
| Foregene Protease Plus |
| DNA-Chete Column |
| Mirayiridzo |
Zvimiro&zvakanakira
-Kwete kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva kune genomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
-Kukurumidza kukurumidza: Foregene Protease Plus ine basa rakakwirira kudarika maprotease akafanana, uye inogaya sampuli dzenyama nokukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me40 maminetsi.
-Yakanaka: Iyo centrifugation inoitwa pane tembiricha yekamuri, uye hapana chikonzero che4 ° C yakaderera-kupisa centrifugation kana ethanol precipitation yeDNA.
-Kuchengeteka: Hapana organic reagent kutorwa kunodiwa.
-Hunhu hwepamusoro: Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
Kit application
Inokodzera kubviswa uye kucheneswa kwegenomic DNA kubva kutsva kana chando hombe uye mbeva miswe (inoshandiswawo kuchenesa mbeva kana makonzo tsandanyama uye mbeva nzeve genomic DNA).
Workflow
Storage uye Sherufu upenyu
-Iyi kit inogona kuchengetwa kwemwedzi gumi nemiviri pasi pemamiriro akaoma panzvimbo yekupisa (15-25 ° C);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8°C.
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.
-Forgene Protease Plus mhinduro ine chimiro chakasiyana-siyana, inoshanda kana ichichengetwa mukamuri yekushisa kwenguva yakareba (mwedzi mitatu);yakachengetwa pa4 ° C, basa uye kugadzikana zvichava nani, saka zvinokurudzirwa kuichengeta pa4 ° C, yeuka kuti usaichengeta pa -20 ° C.
