RT Easyᵀᴹ I Master Premix yekutanga-strand cDNA synthesis
Kit Description:
-Inoshanda reverse transcription system, zvinongotora maminetsi makumi maviri neshanu kupedzisa synthesis yekutanga strand cDNA.
-Yakakwirira-sensitivity reverse transcription system, pg-level matemplate anogonawo kuwana yakakwirira-mhando cDNA.
- Iyo reverse transcription system ine yakanyanya kugadzikana yekupisa, iyo tembiricha yemaitiro inogona kunge yakakwira kusvika 50 ℃,uye ine yakanaka reverse transcription performance.
-Kunyangwe masampuli eRNA asina kuchena (doro rinosvika 45%, guanidine munyu unosvika 750mM) inogona kuitiswa reverse transcription reaction.
Kit Descriptions
FOREGENE's 2 × RT EasyTMMusanganiswa unogona kubatanidza iyo yekutanga-strand cDNA patembiricha yakakwira kusvika 50 ° C, iyo inobatsira kune reverse transcription reaction yeyakaoma yechipiri chimiro RNA matemplate.
Iyo RNA yakacheneswa murabhoritari kazhinji ine doro uye guanidine munyu zvisaririra, izvo zvine simba inhibitory properties pane akawanda reverse transcriptases, zvichikonzera zvisingagutsi reverse transcription effects kana yakaderera reverse transcription performance.FOREGENE 2 × RT NyoreTMMusanganiswa unoratidza kushivirira kwakanyanya kudoro uye guanidine munyu, yakanyanya kushivirira doro mumasampuli eRNA i45%, uye yakanyanya kushivirira kune guanidine munyu i750mM.Kunyangwe iyo RNA isina kuchena, iyo reverse transcription reaction inogona kuitwa ne FORREGENE 2 × RT Musanganiswa.Iyo yakasarudzika reaction system inoita kuti kuita kweRT kuve nyore, nekukurumidza uye kushanda nesimba, uye synthesis yekutanga strand cDNA inogona kupedzwa mumaminetsi makumi maviri nemashanu.
Ichi chigadzirwa chinogona kushandiswa pamwe chete neForegene's fluorescent quantitative chigadzirwa Chaiyo Nguva PCR Nyore.TMkuti uwane mhinduro dzemhando yepamusoro dzekuyedza.
Zvinotsanangurwa
| RT NyoreTMI Master Premix yekutanga-strand cDNA synthesis | ||
| Kit zviri mukati | RT-01011 | RT-01012 |
| 25 nguva (20μl system) | 100 nguva (20μl system) | |
| 2× RT EasyTM Mix | 0.25ml | 1ml |
| Random Primer (50μM) | 50μl | 200μl |
| Oligo(dT)18 Primer (50μM) | 50μl | 200μl |
| RNase-Yemahara ddH2O | 1.7ml | 1.7ml |
| Mirayiridzo Manual | 1 chidimbu | 1 chidimbu |
Zvimiro&zvakanakira
-Inoshanda reverse transcription system, zvinongotora maminetsi makumi maviri neshanu kupedzisa synthesis yekutanga strand cDNA.
-Yakakwirira-sensitivity reverse transcription system, pg-level matemplate anogonawo kuwana yakakwirira-mhando cDNA.
-Iyo reverse transcription system ine yakanyanya kugadzikana yekupisa, tembiricha yemaitiro inogona kunge yakakwira kusvika 50 ℃, uye ine yakanaka reverse transcription performance.
-Kunyangwe masampuli eRNA asina kuchena (doro rinosvika 45%, guanidine munyu unosvika 750mM) inogona kuitiswa reverse transcription reaction.
Kit application
- Yakajairika RT-PCR
- Synthesis ye cDNA yecloning uye yekutaura tsvagiridzo
-Ongororo yenzira yekuwedzera yenzvimbo yekutanga kunyorera
- RACE (kukurumidza kukwidziridzwa kwecDNA kupera)
- Linear RNA kukwidziridzwa
- Chip chiratidzo
Kuyerera kwebasa
Kuchengetedza uye hupenyu hwesherufu
1.Mamiriro ekufambisa
Iyo yese maitiro ekudzika-tembiricha echando bhokisi chekufambisa, kuve nechokwadi chekuti kit iri munzvimbo ye <4 ℃.
2. Mamiriro ekuchengetedza
RT NyoreTMNdinochengetwa pa -20°C.Chengetedza chigadzirwa mufiriji yekushisa nguva dzose pa -20 ° C pakarepo mushure mekugamuchira.Kana mamiriro ekuchengetedza akakodzera, chigadzirwa hachizodzikisi chero mashandiro panguva ye1-gore yechokwadi nguva.
