China Mouse Muswe Wakananga PCR Kit-UNG Yakananga PCR Lysis Reagent(Mouse Muswe)(yeGenotyping) Mugadziri uye Mutengesi |Foregene

Mouse Muswe Wakananga PCR Kit-UNG Yakananga PCR Lysis Reagent(Mouse Muswe)(yeGenotyping)

Kit Description:

Iyo mbeva muswe lysate inoshandiswa zvakananga se template yePCR kukwidziridzwa pasina yakaparadzana kudhirowa kweDNA, iyo inokurumidza uye yakanyanya kunzwa.Yakawedzera UNG anti-kusvibisa sisitimu yekubvisa manyepo.

◮Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.

◮Sample inodiwa idiki, ingotora mhodzi imwe chete.

◮Hapana mishonga yakakosha yakadai sekugaya nekupwanya inodiwa, uye kushanda kuri nyore.

◮Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.

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Product Detail

Product Tags

Tsanangudzo

Iyi kit inoshandisa yakasarudzika lysis buffer system kukurumidza kuburitsa genomic DNA kubva kune mbeva miswe, nzeve, tsandanyama uye mamwe matissue samples ePCR reactions, saka inonyanya kukodzera kuongororwa kwakakura kwemajini.

Iyo nzira yekuburitsa genomic DNA kubva kulysis buffer inopedzwa mukati me10-30 maminetsi pa65 ° C.Hapana mamwe maitiro akadai seprotein uye RNA kubviswa anodiwa, uye yakaburitswa trace DNA inogona kushandiswa setemplate yePCR reaction.

2 × M1-PCR Nyore^TMMix (UNG) inoshandisa dUTP pachinzvimbo che dTTP pahwaro hwe2 × M1-PCR Easy^TMSanganisa (UNG), uye anowedzera UNG enzyme (Uracil-N-glycosylase) iyo inogona kukanganisa template ine dUTP panguva imwe chete.Pamberi pePCR maitiro, iyo UNG enzyme inoshandiswa kusvibisa PCR chigadzirwa chine uracil.Iyo UNG enzyme haizove nemhedzisiro pane template iyo isina uracil, nekudaro ichiva nechokwadi cheiyo chaiyo uye kurongeka kweamplification uye kudzivirira mukana weiyo yakakura-chiyero genetic test.Kusvibiswa kwePCR zvigadzirwa kwakaitika.

D-Taq DNA polymerase ndeye DNA polymerase yakanyatso gadzirwa neForegene kune yakananga PCR maitiro.D-Taq DNA polymerase ine kushivirira kwakasimba kune akasiyana PCR reaction inhibitors, uye inokwanisa kunyatso kukwidziridza huwandu hweDNA mune dzakasiyana siyana dzakaomarara maitiro masisitimu, uye iyo amplification kumhanya inogona kusvika 2Kb/min, iyo inonyanya kukodzera Direct PCR reaction.

Zvinotsanangurwa

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Kit zvikamu

Chikamu I	Buffer MP
	Foregene Protease Plus
	6 × DNA Loading Buffer
Chikamu II	2 × M1 PCR Nyore^TMMusanganiswa(UNG)
Mirayiridzo

Zvimiro&zvakanakira

■Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.

∎ Sample inodikanwa idiki, zvishoma se5mg mbeva tishu kana 2-5mm muswe wembeva inogona kuongororwa.

∎ Hapana mishonga yakakosha yakadai sekukuya nekupwanya inodiwa, uye kuvhiya kuri nyore.

■ Iyo yakagadziridzwa PCR sisitimu inoita kuti PCR ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.

■ Anti-kusvibiswa PCR system 2×M1-PCR Nyore^TMSanganisa (UNG), iyo inobvisa zvinobudirira kusvibiswa kunokonzerwa nePCR zvigadzirwa uye inova nechokwadi cheiyo chaiyo uye nekururama kweamplification.

Kit parameters

Kuwanda kwekushandisa: muswe wembeva, nzeve yembeva, tsandanyama uye mamwe matishu.

DNA yakaburitswa kubva kumuenzaniso lysis: inoshandiswa chete sePCR template.

Iyo kit inogona kushandiswa kune zvinotevera zvinangwa: kuzivikanwa kwe transgenosis, mhuka genotyping, nezvimwe.

Kuyerera kwebasa

Storage uye Sherufu upenyu

Chikamu I chekiti ichi chinofanira kuchengetwa pa2-8 ℃.

Iyo reagent Buffer AL inogona kuchengetwa kwemwedzi gumi nemaviri pasi pemamiriro akaoma;inogona kuchengetwa pa2-8 ℃ kuitira kuchengetedza kwenguva refu.

Reagent Foregene Protease ine yakasarudzika fomula.Kuti ive nechokwadi chekuita kwayo uye kugadzikana, ndapota chengeta pa4 ℃ kwemwedzi gumi nemiviri.

Reagent 6 × DNA Loading Buffer inogona kuchengetwa pa4 ℃ kana -20 ℃ kwenguva yakareba.

Chikamu II chekiti ichi chinofanira kuchengetwa pa -20 ℃.

Reagent 2 × M1 PCR Easy TM Mix (UNG), kana inoshandiswa kakawanda, inogonawo kuchengetwa pa4 ℃ yekuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi).

Zvakapfuura: Mouse Muswe SuperDirect PCR Kit Yakananga PCR Lysis Reagent(Mouse Muswe)(yeGenotyping)

Zvinotevera: Mouse Muswe Wakananga PCR Kit Yakananga PCR Lysis Reagent (Mouse Muswe) (yeGenotyping)

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