China Mbizi Hove Yakananga PCR Kit-UNG Yakananga PCR Lysis Reagent(zebrafish) Mugadziri uye Mutengesi |Foregene

Mbizi Hove Yakananga PCR Kit-UNG Yakananga PCR Lysis Reagent(zebrafish)

Kit Description:

Kushandiswa kwakananga kwezebrafish lysate se template yePCR amplification, hapana kubviswa kwakasiyana kweDNA, nekukurumidza uye kwakanyanya kunzwa.Yakawedzera UNG anti-kusvibisa sisitimu yekubvisa manyepo.

◮Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.

◮Sample inodiwa idiki, ingotora mhodzi imwe chete.

◮Hapana mishonga yakakosha yakadai sekugaya nekupwanya inodiwa, uye kushanda kuri nyore.

◮Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.

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Product Detail

Product Tags

Tsanangudzo

Iyi kit inoshandisa yakasarudzika lysis buffer system kukurumidza kuburitsa genomic DNA kubva kuzebrafish uye mamwe matishu ehove yemvura yakachena, zvimbi zvemuswe kana mazai ehove samples for PCR reactions, saka inonyanya kukodzera kuongororwa kwakakura kwemajini.Iyo nzira yekuburitsa genomic DNA kubva kulysis buffer inopedzwa mukati me10-30 maminetsi pa65 ° C.Hapana mamwe maitiro akadai seprotein uye RNA kubviswa anodiwa, uye yakaburitswa trace DNA inogona kushandiswa setemplate yePCR reaction.

2 × PCR Easy TM Mix (UNG) inoshandisa dUTP panzvimbo ye dTTP pamusana pe2 × PCR EasyTM Mix, uye inowedzera UNG enzyme (Uracil-N-glycosylase) iyo inogona kukanganisa template ine dUTP panguva imwe chete.Pamberi pePCR maitiro, iyo UNG enzyme inoshandiswa kusvibisa PCR chigadzirwa chine uracil.Iyo UNG enzyme haizove nemhedzisiro pane template iyo isina uracil, nekudaro ichiva nechokwadi cheiyo chaiyo uye kurongeka kweamplification uye kudzivirira mukana weiyo yakakura-chiyero genetic test.Kusvibiswa kwePCR zvigadzirwa kwakaitika.

D-Taq DNA polymerase ndeye DNA polymerase yakanyatso gadzirwa neForegene kune yakananga PCR maitiro.D-Taq DNA polymerase ine kushivirira kwakasimba kune akasiyana PCR reaction inhibitors, uye inokwanisa kunyatso kukwidziridza huwandu hweDNA mune dzakasiyana siyana dzakaomarara maitiro masisitimu, uye iyo amplification kumhanya inogona kusvika 2Kb/min, iyo inonyanya kukodzera Direct PCR reaction.

Zvinotsanangurwa

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Kit zvikamu

Chikamu I	Buffer FP
	Foregene Protease
	6 × DNA Loading Buffer
Chikamu II	2 × PCR Nyore^TMMusanganiswa(UNG)
Mirayiridzo

Zvimiro&zvakanakira

■ Hapana kunotora nguva uye kunodhura kuchenesa DNA kunodiwa.

∎ Sample inodikanwa idiki, zvishoma se1mg hove caudal fin kana 10 mazai ehove anogona kuongororwa.

∎ Hapana mishonga yakakosha yakadai sekukuya nekupwanya inodiwa, uye kuvhiya kuri nyore.

■ Anti-kusvibiswa PCR system 2 × PCR Easy TM Mix (UNG), iyo inogona kunyatsobvisa kusvibiswa kunokonzerwa nePCR zvigadzirwa uye kuvimbisa kujeka uye kururamisa kwekusimudzira.

■ Optimized PCR system inoita kuti PCR ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.

Kit parameters

Kushandisa: Hove dzeZebra nedzimwe hove dzemvura yakachena.

DNA yakaburitswa kubva kumuenzaniso lysis: inoshandiswa chete sePCR template.

Iyo kit inogona kushandiswa kune zvinotevera zvinangwa: kuzivikanwa kwe transgenosis, mhuka genotyping, nezvimwe.

Workflow

Storage uye Sherufu upenyu

Chikamu I chekiti ichi chinofanira kuchengetwa pa2-8 ℃.

Iyo reagent Buffer FP inogona kuchengetwa kwemwedzi gumi nemiviri pasi pemamiriro akaoma;inogona kuchengetwa pa2-8 ℃ kuitira kuchengetedza kwenguva refu.

v Reagent Forgene Protease ine yakasarudzika fomula.Kuti ive nechokwadi chekuita kwayo uye kugadzikana, ndapota chengeta pa4 ℃ kwemwedzi gumi nemiviri.

vReagent 6 × DNA Loading Buffer inogona kuchengetwa pa4 ℃ kana -20 ℃ kwenguva yakareba.

Chikamu II chekiti ichi chinofanira kuchengetwa pa -20 ℃.

vReagent 2 × PCR Easy TM Mix (UNG), kana yakashandiswa kakawanda, inogonawo kuchengetwa pa4 ℃ yekuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi).

Zvakapfuura: Mbizi Hove Yakananga PCR Kit Yakananga PCR Lysis Reagent (mbizi)

Zvinotevera: Mouse Muswe SuperDirect PCR Kit Yakananga PCR Lysis Reagent(Mouse Muswe)(yeGenotyping)

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