China Mbizi Hove Yakananga PCR Kit Yakananga PCR Lysis Reagent(zebrafish) Manufacturer and Supplier |Foregene

Mbizi Hove Yakananga PCR Kit Yakananga PCR Lysis Reagent (mbizi)

Zebra Hove Yakananga PCR Kit Yakananga PCR Lysis Reagent(zebrafish) Featured Image

Kit Description:

Shandisa zvakananga zebrafish lysate se template yePCR amplification, pasina kubviswa kwakasiyana kweDNA, inokurumidza uye yakanyanya kunzwa.

◮Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.

◮Sample inodiwa idiki, ingotora mhodzi imwe chete.

◮Hapana mishonga yakakosha yakadai sekugaya nekupwanya inodiwa, uye kushanda kuri nyore.

◮Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.

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Product Detail

Product Tags

Tsanangudzo

Ichi chigadzirwa chinoshandisa yakasarudzika lysis buffer system kukurumidza kuburitsa genomic DNA kubva kuzebrafish uye mamwe matishu ehove yemvura yakachena, zvimbi zvemuswe kana mazai ehove samples for PCR reactions, saka inonyanya kukodzera kuongororwa kwakakura kwemajini.Iyo nzira yekuburitsa genomic DNA kubva kulysis buffer inopedzwa mukati me10-30 maminetsi pa65 ° C.Hapana mamwe maitiro akadai seprotein uye RNA kubviswa anodiwa, uye yakaburitswa trace DNA inogona kushandiswa setemplate yePCR reaction.

2 × PCR Nyore^TMMusanganiswa une kushivirira kwakasimba kuPCR reaction inhibitors, uye inogona kushandisa lysate yemuenzaniso kuti iongororwe setemplate yeanoshanda uye chaiwo amplification.Iyi reagent ine ForegeneD-Taq DNAPolymerase, dNTPs, MgCl.₂, reaction buffer, PCR optimizer uye stabilizer.Inoshandiswa pamwe chete ne lysis buffer inogona kukurumidza uye nyore kuona samples, uye ine hunhu hwekunzwa kwepamusoro, kusimba kwakasimba, uye kugadzikana kwakanaka.

D-Taq DNA polymerase ndeye DNA polymerase yakanyatso gadzirwa neForegene kune yakananga PCR maitiro.D-Taq DNA polymerase ine kushivirira kwakasimba kune akasiyana PCR reaction inhibitors, uye inokwanisa kunyatso kukwidziridza huwandu hweDNA mune dzakasiyana siyana dzakaomarara maitiro masisitimu, uye iyo amplification kumhanya inogona kusvika 2Kb/min, iyo inonyanya kukodzera Direct PCR reaction.

Zvinotsanangurwa

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Kit zvikamu

Chikamu I	Buffer FP
	Foregene Protease
	6 × DNA Loading Buffer
Chikamu II	2 × PCR Nyore^TMMix
Mirayiridzo

Zvimiro&zvakanakira

■Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.

∎ Sample inodikanwa idiki, zvishoma se1mg hove caudal fin kana 10 mazai ehove anogona kuongororwa.

∎ Hapana mishonga yakakosha yakadai sekukuya nekupwanya inodiwa, uye kuvhiya kuri nyore.

■ Optimized PCR system inoita kuti PCR ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.

Kit parameters

Kushandisa: Hove dzeZebra nedzimwe hove dzemvura yakachena.

DNA yakaburitswa kubva kumuenzaniso lysis: inoshandiswa chete sePCR template.

Iyo kit inogona kushandiswa kune zvinotevera zvinangwa: kuzivikanwa kwe transgenosis, mhuka genotyping, nezvimwe.

Kuyerera kwebasa

Storage uye Sherufu upenyu

Chikamu I chekiti ichi chinofanira kuchengetwa pa2-8 ℃.

Iyo reagent Buffer FP inogona kuchengetwa kwemwedzi gumi nemiviri pasi pemamiriro akaoma;inogona kuchengetwa pa2-8 ℃ kuitira kuchengetedza kwenguva refu.

v Reagent Forgene Protease ine yakasarudzika fomula.Kuti ive nechokwadi chekuita kwayo uye kugadzikana, ndapota chengeta pa4 ℃ kwemwedzi gumi nemiviri.

vReagent 6 × DNA Loading Buffer inogona kuchengetwa pa4 ℃ kana -20 ℃ kwenguva yakareba.

Chikamu II chekiti ichi chinofanira kuchengetwa pa -20 ℃.

vReagent 2 × PCR Easy TM Mix, kana inoshandiswa kakawanda, inogonawo kuchengetwa pa 4 ℃ yekuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi).

Zvakapfuura: ForeSNP Genotyping Kit

Zvinotevera: Mbizi Hove Yakananga PCR Kit-UNG Yakananga PCR Lysis Reagent(zebrafish)

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