Foregene DNA Identification System 38Y-C (Nyaya)
Kit Description:
Foregene DNA identification system 38Y-C inoshandisa tekinoroji yemavara matanhatu-ruvara rwefluorescent kukudza 38 Y chromosome STR nzvimbo, 3 Y-Indel lociuyemaviri emukati quality control loci panguva imwe cheteyeamplification Case samples yakatorwa kubva kucheneswa DNA matemplate.
Tsanangudzo
Foregene DNA identification system 38Y-C inoshandisa tekinoroji yemavara matanhatu-ruvara rwefluorescent kukudza 38 Y chromosome STR nzvimbo, 3 Y-Indel lociuyemaviri emukati quality control loci panguva imwe cheteyeamplification Case samples yakatorwa kubva kucheneswa DNA matemplate.
Kit Content
Amplification system kugadzirira
Thekugadzirira composition of Standardkutorwauye amplification system
| Master Mix | Cvanopikisa | 10μl system (μl) |
| 2.5×PCR Master Mix C I | 4.0 | |
| 5 x 38Y-C Primer Mix | 2.0 | |
| DNA template | 1- 4 | |
| Deionized mvura | Zadza kusvika kureaction volume ye10 μl |
Data Analysis
Yakawedzerwa Girafu 1:Allelic Ladder Typing Map
Yakawedzerwa Girafu 2:DNA kutaipa standard 9948 kutaipamap
Reagent Storage
1. Ndapota chengetedza pasi -20 ° C (kana isina kushandiswa kwenguva pfupi), mushure mokugamuchira makiti akaomeswa muchando chakaoma kana gel ice packs.
2. Ndapota sotre the pre-reaction component PCR master mix iri pa -20 ° C, mushure mokunge kititi chatorwa kuti chishandiswe uye zvakasara zvakasara zvinogadziriswa zvinochengetwa pa 4 ° C kuti zvirege kudzokorora kutonhora uye kunyunguduka.Kana iyo imwe dosage iri diki, inokurudzirwa kuchengeta pa -20 ° C mushure mealiquoting.
3. Chengetedza zvikamu mushure mekuita pa 4 ° C, dzivisa kudzokorora kutonhora uye kunyunguduka, uye usagunzva reagents musati waita kuti urege kusvibiswa.
