Foregene DNA Identification System 30 AC (Nyaya)
Kit Description:
Foregene DNA identification system30A-C inoshandisa tekinoroji yemavara matanhatu-ruvara rwefluorescent kutikuwedzera 29autosomalSTR loci, 1 Amel locus, 1 Yindellocus, uye1 nzvimbo dzevanhu dzakananga dzeBonde, 2yemukati yemhando yekudzora nzvimbo, inoshandiswa kukwidziridza iyoDNAtemplate yakabviswa uye yakacheneswa kubvaCase zvinhu.
Tsanangudzo
Foregene DNA identification system30A-C inoshandisa tekinoroji yemavara matanhatu-ruvara rwefluorescent kutikuwedzera 29autosomalSTR loci, 1 Amel locus, 1 Yindellocus, uye1 nzvimbo dzevanhu dzakananga dzeBonde, 2yemukati yemhando yekudzora nzvimbo, inoshandiswa kukwidziridza iyoDNAtemplate yakabviswa uye yakacheneswa kubvaCase zvinhu.
Kit Content
Amplification system kugadzirira
Tafura 2: Iyo Kugadzirira Kuumbwa kweiyo Yakajairwa kudhirowa uye amplification system
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| Cvanopikisa | 25ul system (µl) | 10μl system (μl) |
| Master Mix | 2.5 × PremixCⅡ | 10.0 | 4.0 |
| 5 x 30 AC Primer Mix | 5.0 | 2.0 | |
| DNA template | 2.5-10 | 1-4 | |
| Deionized mvura | Ita kusvika kuvhoriyamu yekupindura ye25μl | Gadzirisai marection volume ye10 μl |
Data Analysis
Yakawedzerwa Girafu 1:Allelic Ladder Typing Map
Yakawedzerwa Girafu 2:DNA kutaipa standard 9948 kutaipamap
Reagent Storage
1. Ndapota chengetedza pasi -20 ° C, mushure mokugamuchira makiti akaomeswa muchando chakaoma kana gel ice packs.
2. Ndapota chengetedza chikamu che pre-reaction component 2.5 × premix CII pa -20 ° C mushure mokunge kititi ichitorwa kunze kuti chishandiswe, uye zvakasara zvakasara zvinogadziriswa zvakachengetwa pa4 ° C kuti zvirege kudzokorora kutonhora uye kunyunguduka.Kana iyo imwe dosage iri diki, inokurudzirwa kuchengeta pa -20 ° C mushure mealiquoting.
3. Chengetedza zvikamu mushure mekuita pa 4 ° C, dzivisa kudzokorora kutonhora uye kunyunguduka, uye usagunzva reagents musati waita kuti urege kusvibiswa.
