Foregene DNA Identification System Y Plus (Isiri-Kubvisa)
Kit Description:
ForegeneDNA identification system Y Plus inoshandisa tekinoroji yemavara matanhatu efluorescent kukudza 20 Y chromosome core loci, 15 inodiwa loci, 6 imwe nzvimbo loci uye 3 Y indel loci panguva imwe..
Tsanangudzo
ForegeneDNA identification system Y Plus inoshandisa tekinoroji yemavara matanhatu efluorescent kukudza 20 Y chromosome core loci, 15 inodiwa loci, 6 imwe nzvimbo loci uye 3 Y indel loci panguva imwe..
Kit Content
Amplification system kugadzirira
Thekugadzirira kuumbwazve sstandard(isina kubviswa)amplification system
| Cvanopikisa | 25 μlsystem (μl) | 10μl system (μl) | |
| Master Mix | 4 × Master MixVII | 6.25 | 2.5 |
| 5×Y Plus Primer Mix | 5.0 | 2.0 | |
| Deionized mvura | 13.75 | 5.5 | |
| Blood tsvina | Diameter 1.2mm | Diameter 1.2mm | |
| Total reaction volume | 25 μl | 10 μl | |
Data Analysis
Yakawedzerwa Girafu 1:Allelic Ladder Typing Map
Yakawedzerwa Girafu 2:DNA kutaipa standard 9948 kutaipamap
Reagent Storage
1. Ndapota chengetedza pasi -20 ° C (Kana isina kushandiswa kwenguva pfupi), mushure mokugamuchira makiti akaomeswa nechando muchando chakaoma kana gel ice packs.
2. Ndapota chengetedza pre-reaction component 4 × premix VII pa -20 ° C, mushure mokunge kititi chatorwa kunze kuti chishandiswe, uye mamwe akasara e-pre-reaction reagents anochengetwa pa 4 ° C kuti arege kudzokorora kutonhora uye kunyunguduka.Kana iyo imwe dosage iri diki, inokurudzirwa kuchengeta pa -20 ° C mushure mealiquoting.
3. Chengetedza zvikamu mushure mekuita pa 4 ° C, dzivisa kudzokorora kutonhora uye kunyunguduka, uye usagunzva reagents musati waita kuti urege kusvibiswa.
