Ropa RNA Isolation Kit
Kit Description:
Katsi.No.RE-04011/04013
Kucheneswa kwakazara kweRNA kubva muropa rose 104 ≤ Masero machena eropa ≤ 107
Kurumidza kuparadzanisa uye kuchenesa ropa RNA kubva kumasero machena eropa.
-Hapana chikonzero chekunetseka nezveRNA kuderedzwa.Iyo yese kit ndeye RNase-Yemahara
-Zviri nyore-mabasa ese anopedzwa patembiricha yekamuri
-Kukurumidza-kushanda kunogona kupedzwa mumaminitsi makumi maviri
-Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatso chenesa RNA
-Yakachengeteka-hapana organic reagent inoshandiswa
-Kukura sampuli yekugadzirisa simba-kusvika ku200μl samples inogona kugadziriswa nguva imwe neimwe.
-Hunhu hwepamusoro-iyo RNA yakacheneswa yakanyanya kuchena, isina mapuroteni uye kumwe kusvibiswa, uye inogona kusangana neyakasiyana-siyana yekudzika yekuyedza maapplication.
Tsanangudzo
Iyo kit inotora iyo spin column uye fomula yakagadziridzwa nekambani yedu, iyo inogona kunyatso bvisa yakakwira-kuchena uye yemhando yepamusoro RNA kubva kune anticoagulated ropa rose.Iyo kiti inopa tsvuku tsvuku yeropa lysate (Buffer RCL), iyo inogona kukurumidza uye zvinobudirira lyse masero matsvuku eropa uye kuchengetedza chena masero eropa.Iyo inoshanda DNA-Kuchenesa Colunm inogona nyore kuparadzanisa supernatant uye sero lysates uye adsorb uye kubvisa genomic DNA.Iko kushanda kuri nyore uye kuchengetedza nguva;Iyo RNA-chete Column inokwanisa kusunga RNA zvine mutsindo, uye neyakasarudzika fomula, inogona kugadzirisa nhamba yakakura yemasampuli panguva imwe chete.
Iyo yese system RNase-Yemahara inoita iyo yakabviswa RNA isaparare;Buffer RW1 uye Buffer RW2 buffer washing system inoita iyo inowanikwa RNA isina mapuroteni, DNA, ions uye organic compound kusvibiswa.
Kit Zviri mukati
| Ropa Rakazara RNA Isolation Kit | ||
| Kit composition | RE-04011 | RE-04013 |
| 50 nguva | 200 nguva | |
| Buffer RCL (10×) | 52.5 mL | 210mL |
| Buffer BRL1* | 30mL | 120mL |
| Buffer BRL2 | 18mL | 66mL |
| Buffer RW1* | 25mL | 100mL |
| Buffer RW2 | 24mL | 96mL |
| RNase-Yemahara ddH2 O | 10mL | 40mL |
| RNA-chete Column | 50 seti | 200 seti |
| DNA-Kuchenesa Column | 50 seti | 200 seti |
| manual | 1 kopi | 1 kopi |
Zvimiro&zvakanakira
-Hapana chikonzero chekunetseka nezveRNA kuderedzwa.Iyo yese kit ndeye RNase-Yemahara.
-Zviri nyore-mabasa ese anopedzwa patembiricha yekamuri.
-Kukurumidza-kushanda kunogona kupedzwa mumaminitsi makumi maviri.
-Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatso chenesa RNA.
-Yakachengeteka-hapana organic reagent inoshandiswa.
-Kukura sampuli yekugadzirisa simba-kusvika ku200μl samples inogona kugadziriswa nguva imwe neimwe.
-Hunhu hwepamusoro-iyo RNA yakacheneswa yakanyanya kuchena, isina mapuroteni uye kumwe kusvibiswa, uye inogona kusangana neyakasiyana-siyana yekudzika yekuyedza maapplication.
Kit parameters
Kit application:
Inokodzera kubviswa uye kucheneswa kweiyo RNA yese kubva muropa remammalian rakazara.
Kuyerera kwebasa
Kuchengetedza mamiriro
Buffer RCL (10 ×) inofanira kuchengetwa pa 2-8 ℃;zvimwe zvikamu zvekiti zvinogona kuchengetwa pakamuri tembiricha (15-25 ℃) pasi pemamiriro akaoma, uye inogona kuchengetwa kwemwedzi gumi nemiviri.Buffer BRL1 inogona kuchengetwa pa 4 ℃ kwemwedzi 1 mushure mekuwedzera β-mercaptoethanol (kusarudza) .
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati isati yashandiswa.Kana zvichidikanwa, preheat mubhati remvura 37 ° C kwemaminitsi gumi kuti uparadze mvura, uye sanganisa zvakanaka usati washandisa.
Nhungamiro dzekuongorora matambudziko
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
Hapana RNA inogona kutorwa kana goho re nucleic acid rakaderera
Pane kazhinji zvinhu zvakawanda zvinokanganisa kudzoreredza kushanda zvakanaka, senge: sampuli RNA yemukati, nzira yekushanda, elution vhoriyamu, nezvimwe..
Kuongorora kwezvikonzero zvinowanzoitika:
1.Ice kugeza kana kuderera-kupisa (4 ° C) centrifugation panguva yekushanda.
Zano: Tembiricha yekamuri (15-25 ° C) mashandiro, usambofa kugeza nechando uye yakaderera tembiricha centrifuge.
2. Zvisizvo sampuli yekuchengetedza kana sampuli yekuchengetedza kwenguva yakareba.
Zano: Chengetedza sampuli pa -80 ° C kana kuomesa munitrogen yemvura, uye dzivirira kudzokororwa kushandiswa kwechando-nyoro;edza kushandisa masampuli achangobva kuunganidzwa eRNA kutorwa.
3.Isina kukwana sampuli lysis
Kurudziro: Ndokumbira utarise kuti sampuli uye mhinduro yekushanda (Linear Acrylamide) yakanyatso kusanganiswa uye yakanamirwa kwemaminetsi gumi patembiricha yekamuri (15-25 ° C)
4.The eluent yakawedzerwa zvisizvo
Kurudziro: Ita shuwa kuti RNase-Yemahara ddH2O inowedzerwa pakati pe membrane yekoramu yekuchenesa.
5.Improper volume yeanhydrous ethanol muBuffer viRW2
Zano: Ndokumbira uteedzere mirairo, wedzera iyo chaiyo vhoriyamu yeanhydrous ethanol kuBuffer viRW2 wosanganisa zvakanaka usati washandisa kiti.
6.Improper sampuli kushandiswa.
Zano: 200µl yesampuli pa500μl yeBuffer viRL.Yakawandisa sampuli vhoriyamu inokonzeresa kuderedzwa kweRNA yekubvisa mwero.
7.Improper elution vhoriyamu kana kusakwana elution.
Zano: Huwandu hwakajeka hwekoramu yekuchenesa ndeye 30-50μl;kana iyo elution effect isingagutsi, zvinokurudzirwa kuwedzera pre-heated RNase-Mahara ddH.2O uye wedzera nguva yekuisa pane tembiricha yekamuri, senge 5-10min
8.Kunatsa koramu ine ethanol yasara mushure mekugezeswa muBuffer viRW2.
Zano: Kana ethanol ichiri kusara mushure mekugezeswa muBuffer viRW2 uye isina chinhu-chubhu centrifugation kwe2min, mbiru yekunatsa inogona kusiiwa patembiricha yekamuri kwemaminetsi mashanu mushure mekunge isina chinhu-chubhu centrifugation kubvisa zvachose ethanol yasara.
Kushatiswa kwemamorekuru eRNA akacheneswa
Hunhu hweRNA yakacheneswa hunoenderana nezvinhu zvakaita seyekuchengetedza sampuli, kusvibiswa kweRNase, uye kushanda.
Kuongorora kwezvikonzero zvinowanzoitika:
1.Masampuli akaunganidzwa haana kuchengetwa munguva.
Zano: Kana sampu ikasashandiswa nenguva mushure mekuunganidzwa, ndapota ichengetedze pa -80 ℃ kana mvura yenitrogen nekukasira.Pakubviswa kwemamorekuru eRNA, edza kushandisa masampuli achangobva kuunganidzwa pese pazvinogoneka.
2.Kuunganidza samples kwaive kutonhora uye kunyunguduka kakawanda.
Zano: Dzivisa kudzokorora kutonhora nekunyunguduka (kwete kanopfuura kamwe) panguva yekuunganidza nekuchengetedza sampuli, zvikasadaro goho re nucleic acid richadzikira.
3.RNase yakaunzwa muimba yekushanda kana pasina magurovhosi anoraswa, masiki, nezvimwe zvakapfekwa.
Zano: Kubviswa kweRNA mamorekuru kuyedza kunoitwa zvakanyanya mune yakaparadzana RNA kamuri yekuvhiya, uye tafura yekuedzwa inocheneswa isati yaedzwa.Pfeka magurovhosi anoraswa uye masiki panguva yekuyedza kudzivirira kusvibiswa kweRNA kunokonzerwa neRNase sumo.
4.Iyo reagent yakasvibiswa neRNase panguva yekushandiswa.
Zano: Tsiva neViral RNA Isolation Kit itsva yezviedzo zvinoenderana.
5.Kusvibiswa kweRNase kwemachubhu ecentrifuge, matipi epipette, etc. Zano: Iva nechokwadi chekuti machubhu ecentrifuge, matipi epipette, uye mapipette ese ari RNase-Mahara.
Iwo akacheneswa mamorekuru eRNA akakanganisa kuyedza kuzasi
Mamorekuru eRNA akacheneswa nekoramu yekuchenesa achakanganisa kuyedza kuzasi kana paine maion emunyu akawandisa kana mapuroteni, akadai se: reverse transcription, Northern Blot, nezvimwewo.
1.Pane asara ayoni emunyu mune eluted RNA mamorekuru.
Kurudziro: Ita shuwa kuti iyo chaiyo vhoriyamu yeanhydrous ethanol yawedzerwa kuBuffer viRW2, uye geza koramu yekuchenesa kaviri zvinoenderana neiyo chaiyo centrifugation kumhanya pamirayiridzo yekushanda; Kana pachine maion emunyu asara, unogona kuwedzera Buffer viRW2 kukoromo yekuchenesa, uye woisiya pakamuri tembiricha ye5min.Zvadaro ita centrifugation kubvisa salt ions kusvibiswa kusvika pamwero mukuru
2.Pane yasara ethanol mu eluted RNA mamorekuru
Zano: kana uchinge wasimbisa kuti mbiru dzekuchenesa dzakasukwa neBuffer viRW2, ita isina chinhu-chubhu centrifugation zvinoenderana nekumhanya kwecentrifugal pamirayiridzo yekushandisa.Kana kuchine ethanol yasara, inogona kusiiwa kwemaminetsi mashanu pakudziya kwekamuri mushure meisina-tube centrifugation kubvisa yasara ethanol kusvika pamwero mukuru.
Mirayiridzo yezvinyorwa:
Viral RNA Isolation Kit Instruction Manual
