Genomic DNA Micro Kit Micro Genomic DNA Mini Kit
Kit Description:
Kurumidza kuchenesa masampuli madiki kuti uwane yepamusoro-mhando genomic DNA.
◮Hapana RNase kusvibiswa:DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva mujenomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
◮KumhanyaForegene Protease ine chiitiko chepamusoro kupfuura maprotease akafanana, uye inogaya masampula enyama nekukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me20-80 maminetsi.
◮Yakanakira:Iyo centrifugation inoitwa pakamuri tembiricha, uye hapana chikonzero che4 ° C yakaderera-tembiricha centrifugation kana ethanol kunaya kweDNA.
◮Safety:Hapana organic reagent extract inodiwa.
◮Hunhu hwepamusoro:Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
◮Micro-elution system:Inogona kuwedzera kuwanda kweiyo genomic DNA, iyo yakanakira kudzika kwerwizi kana kuyedza.
Tsanangudzo
Kiti iyi inoshandisa DNA-Chete Column iyo inogona kunyatso kusunga DNA, nyowani-itsva Foregene Protease uye yakasarudzika buffer sisitimu, inogona kunyatso tora akasiyana siyana ezvinyorwa zvipenyu, senge huwandu hweropa, maseru, uye mhuka mukati me20-80 maminetsi.Matishu, maronda eropa akaomeswa, kiriniki yekotoni swabs, bvudzi follicles, nezvimwewo, zvinonatswa kuti zviwane yakakwirira-concentration, yepamusoro-mhando genomic DNA.
Zvinotsanangurwa
50 Preps
Kit zvikamu
| Buffer ST1 |
| Buffer ST2* |
| Buffer PW* |
| Buffer WB |
| Buffer EB |
| Foregene Protease |
| DNA-Chete Column |
| Mirayiridzo |
Zvimiro&zvakanakira
-Hapana kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva mujenomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira marabhoritari kuti isasvibiswa neRNase yekunze.
-Kukurumidza kukurumidza: Foregene Protease ine basa rakakwirira kudarika proteases akafanana, uye inochera samples yetishu nokukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me20-80 maminetsi.
-Yakanaka: Iyo centrifugation inoitwa pane tembiricha yekamuri, uye hapana chikonzero che4 ° C yakaderera-kupisa centrifugation kana ethanol precipitation yeDNA.
-Kuchengeteka: Hapana organic reagent kutorwa kunodiwa.
-Hunhu hwepamusoro: Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
-Micro-elution system: Inogona kuwedzera kuwanda kweiyo genomic DNA, iri nyore pakuona kudzika kwemvura kana kuedza.
Kit application
Inokodzera kucheneswa kweanotevera masampuli egenomic DNA: nyowani kana chando mhuka dzenyama, nyowani kana anticoagulated cryopreserved trace yehuwandu hweropa rakazara, makwapa eropa akaomeswa, masero akagadzirwa, microsection tissues, bvudzi follicles, mouthwash, nezvimwe.
Workflow
Storage uye Sherufu upenyu
-Iyi kititi inogona kuchengetwa kwemwedzi gumi nemaviri pasi pemamiriro akaoma pakushambidzika kwekamuri (15-25 ° C);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8°C.
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.
-Forgene Protease mhinduro ine chimiro chakasiyana-siyana, iyo inoshanda kana yakachengetwa mukamuri yekushisa kwenguva yakareba (mwedzi mitatu);basa rayo uye kugadzikana kuchava nani kana yakachengetwa pa4°C, saka zvinokurudzirwa kuchengeta pa4°C, yeuka kusaichengeta pa -20°C.
