FFPE DNA Isolation Kit DNA Extraction Purification Kit kubva kuFFPE Samples
Kit Description:
Kurumidza kuchenesa yemhando yepamusoro genomic DNA kubva muparafini-yakamisikidzwa matishu akadai separafini zvikamu uye mabhuroko eparafini.
◮Hapana RNase kusvibiswa:DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva mujenomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
◮KumhanyaForegene Protease ine chiitiko chepamusoro kupfuura maprotease akafanana, uye inogaya masampula enyama nekukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me20-80 maminetsi.
◮Yakanakira:Iyo centrifugation inoitwa pakamuri tembiricha, uye hapana chikonzero che4 ° C yakaderera-tembiricha centrifugation kana ethanol kunaya kweDNA.
◮Safety:Hapana organic reagent extract inodiwa.
◮Hunhu hwepamusoro:Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
◮Micro-elution system:Inogona kuwedzera kuwanda kweiyo genomic DNA, iyo yakanakira kudzika kwerwizi kana kuyedza.
Tsanangudzo
Iyi kit yakatsaurirwa kubvisa uye kuchenesa yemhando yepamusoro genomic DNA kubva kune formaldehyde-yakagadziriswa tishu kana parafini-yakamisikidzwa tishu.
Iyo genomic DNA yeparafini-yakaiswa sampuli ichave yakasvibiswa nekuda kwenguva yekuchengetedza kana zvakatipoteredza, zvichiita kuti zvive zvakaoma kubvisa genomic DNA.Iyi kit inotora yakasarudzika yakagadziridzwa fomula, iyo inokodzera kutora genomic DNA kubva muparafini-yakamisikidzwa samples ine yakakwira yekubvisa budiriro.Iyo yese maitiro haidi kushandisa chepfu phenol, chloroform kana isopropanol kunaya.
Kiti iyi inoshandisa spin column inogona kunyatsosunga DNA, nyowani-itsva yeForegene Protease Plus, uye yakasarudzika buffer system, inogona kuburitsa yemhando yepamusoro yeparafini-yakamisikidzwa tishu genomic DNA mukati meawa 2-5.
Zvinotsanangurwa
50 Preps, 100 Preps, 250 Preps
Kit zvikamu
| Buffer FL1 |
| Buffer FL2* |
| Buffer PW* |
| Buffer WB |
| Buffer EB |
| Foregene Protease Plus |
| DNA-Chete Column |
| Mirayiridzo |
Zvimiro&zvakanakira
-Kwete kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva kune genomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
-Kukurumidza kukurumidza: Foregene Protease Plus ine basa rakakwirira kudarika maprotease akafanana, uye inogaya sampuli dzenyama nokukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati meawa 2-5.
-Kunakira: Iyo centrifugation inoitwa pakamuri tembiricha, uye hapana chikonzero che4°C pasi-tembiricha centrifugation kana ethanol kunaya kweDNA.
-Kuchengeteka: hapana organic reagent kutorwa kunodiwa.
-Hunhu hwepamusoro: Iyo yakabviswa genomic DNA zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, iyo inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
Kit application
Inokodzera kudhirowa uye kucheneswa kwegenomic DNA kubva kune formaldehyde-yakagadziriswa samples uye parafini-yakamisikidzwa tishu samples.
Workflow
Storage uye Sherufu upenyu
-Iyi kititi inogona kuchengetwa kwemwedzi gumi nemaviri pasi pemamiriro akaoma pakushambidzika kwekamuri (15-25 ° C);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8°C.
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.
-Forgene Protease Plus mhinduro ine yakasarudzika formula inoshanda kana yakachengetwa mukamuri tembiricha kwenguva yakareba (3 mwedzi);inonyanya kushanda uye yakagadzikana kana yakachengetwa pa4 ° C, saka inokurudzirwa kuichengeta pa4 ° C, yeuka kuti usaichengeta pa -20 ° C.
