Foregene DNA Identification System 20A (Yemahara DNA Kutorwa)
Kit Description:
Foregene DNA identification system 20A inoshandisa tekinoroji yemavara mashanu efluorescent kukudza panguva imwe chete 19 STR loci uye 1 sex loci muchubhu imwe.Ndichiri kuvhara gumi nematatu CODIS musimboti loci, makumi maviri epakati loci anosangana nezvinodiwa zveBazi rePublic Security ye autosomal STR kit.Ndiye murume wekurudyi we forensic DNA dhatabhesi yekuvaka uye yekutonga hukama chiziviso.
Tsanangudzo
Foregene DNA identification system 20A inoshandisa tekinoroji yemavara mashanu efluorescent kukudza panguva imwe chete 19 STR loci uye 1 sex loci muchubhu imwe.Ndichiri kuvhara gumi nematatu CODIS musimboti loci, makumi maviri epakati loci anosangana nezvinodiwa zveBazi rePublic Security ye autosomal STR kit.Ndiye murume wekurudyi we forensic DNA dhatabhesi yekuvaka uye yekutonga hukama chiziviso.
Kit Content
| Kit Componet | Zita reComponet | Vhoriyamu (μl / chubhu) | Huwandu (chubhu) |
| PCR Pre-reaction reagent | 2.5 × PCR maitiro buffer Ⅲ | 1000 | 2 |
| 5 × 20A primer musanganiswa | 500 | 2 | |
| Dzora DNA 9947A (1ng/μl) | 25 | 1 | |
| Deionized mvura | 1700 | 2 | |
| Taq Polymerase | Taq polymerase Ⅲ | 40 | 2 |
| PCR Rear-reaction reagent | Allelic ladder20A | 40 | 1 |
| Molecular uremu yemukati standardORG 500 | 150 | 2 | |
| Cherechedza | |||
Data Analysis
1. Vhura iyo Genemapper ID software.Paunenge uchishandisa kit iyi kekutanga, iwe unofanirwa kuendesa mapaneru, bhini yakaenzana, uye saizi inoenderana (uye saizi yakaenzana
2. Ngenisa data ye electrophoresis, sarudza maitiro ekuongorora anowirirana akadai sepaneti, nzira yekuongorora, uye chiyero chekukura, uye shandura rudzi rwemuenzaniso weLadder ku "Allelic Ladder" mu "Sample Type" column;tanga kuongorora data.
Reagent Storage
1. Mushure mekugamuchira kititi chakaoma muchando chakaoma kana plastiki ice packs, kana isingashandisi kwechinguva, tapota chengeta pasi -20 ° C kwenguva yakareba.
2. Mushure mokunge kititi yatorwa uye yakashandiswa, kititi inofanira kuchengetwa pa4 ° C isati yaita kuti irege kudzokorora kutonhora uye kunyunguduka;Taq enzyme inofanira kuchengetwa pa -20 ° C.
3. Mushure mekuita, kititi (kusanganisira molecular weight internal standard and ladder) inofanira kuchengetwa mu "electrophoresis detection room" pa 4 ° C, kudzivisa kudzokorora kutonhora uye kunyunguduka, uye kudzivisa kusangana ne-pre-reaction kit kuti urege kusvibiswa.
