Foregene DNA Identification System 25A-C (Nyaya)
Kit Description:
Foregene DNA Identification System 25A-C inoshandisa tekinoroji yemavara matanhatu efluorescence kukudza 23 autosomal STR loci, imwe Amel loci, imwe Yindel loci uye maviri emukati emhando yekudzora loci panguva yekukwidziridzwa kwematemplate eDNA akatorwa uye akanatswa kubva kune kesi samples.
Tsanangudzo
Foregene DNA Identification System 25A-C inoshandisa tekinoroji yemavara matanhatu efluorescence kukudza 23 autosomal STR loci, imwe Amel loci, imwe Yindel loci uye maviri emukati emhando yekudzora loci panguva yekukwidziridzwa kwematemplate eDNA akatorwa uye akanatswa kubva kune kesi samples.
Kit Content
Amplification system kugadzirira
Iyo Kugadzirira Kuumbwa kweiyo Yakajairwa kudhirowa uye amplification system
| Zvikamu | 10μl system(μl) | |
| Master Mix | 2.5×PCR Premix C Ⅰ | 4.0 |
| 10 × 25A-CPrimer Mix | 1.0 | |
| DNA template | 1—5 | |
| Deionized mvura | Ita kusvika kuhuwandu hwekuita kwe10μl |
Data Analysis
Yakawedzerwa Girafu 1:Ladder genotyping ye allele
Yakawedzerwa Girafu 2:DNA kutaipa standard 9948 kutaipamap
Reagent Storage
1. Mushure mekugamuchira kititi, tapota chengetedza pasi -20 ℃ kana iwe usingashandisi panguva ino.
2. Mushure mekunge kititi yatorwa uye yashandiswa, iyo pre-reaction component PCR premixed solution inochengetwa pa -20 ℃, uye yakasara pre-reaction reagents inochengetwa pa 4 ℃ kuti irege kudzokororwa nechando-thaw.Kana iyo dosi imwe chete idiki, inokurudzirwa kuchengeta pa -20 ° C mushure mekurongedza.
3. Mushure mekuita, zvikamu zvinochengetwa pa 4 ℃ kuti zvirege kudzokororwa nechando-thaw.Usabate pre-reaction reagents kudzivirira kusvibiswa.
