Foregene DNA Identification System 30A (Isiri Kubvisa)
Kit Description:
FOREGENE DNA identification system 30A inoshandisa tekinoroji ine mavara matanhatu efluorescent kukuwedzera 29autosomalSTR loci, PoshiAmel locus uye imwe Yindel locus panguva imwe, uye inogona kukwidziridza zvakananga pepa refirita, FTAmakadhi, machira edonje, makadhi emate,uye mkunze swab.
Tsanangudzo
FOREGENE DNA identification system 30A inoshandisa tekinoroji ine mavara matanhatu efluorescent kukuwedzera 29autosomalSTR loci, PoshiAmel locus uye imwe Yindel locus panguva imwe, uye inogona kukwidziridza zvakananga pepa refirita, FTAmakadhi, machira edonje, makadhi emate,uye mkunze swab.
Kit Content
Amplification System Kugadzirira
Tafura 1: The prepartion composition of Standard (Kubvisa-isina) amplification reaction
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| Cvanopikisa | 25 μl system (μl) | 10 μl system (μl) |
| Master Mix | 4 × Master Mix VII | 6.25 | 2.5 |
| 5 x 30APrimer Mix | 5.0 | 2 | |
| Deionized mvura | 13.75 | 5.5 | |
| Blood tsvina | Diameter 1.2mm | Diameter 1.2mm | |
| Total reaction volume | 25 μl | 10 μl | |
Data Analysis
Yakawedzerwa Girafu 1:Allelic Ladder Typing Map
Yakawedzerwa Girafu 2:DNA kutaipa standard 9948 kutaipamap
Reagent Storage
1. Ndapota chengetedza pasi -20 ° C, mushure mokugamuchira makiti akaomeswa muchando chakaoma kana gel ice packs.
2. Ndapota chengetedza pre-reaction component 4 × premix VII pa -20 ° C, mushure mokunge kititi chatorwa kuti chishandiswe, uye chengetedza zvimwe zvakasara zvakasara zvakagadziriswa pa 4 ° C kuti urege kudzokorora kutonhora nekunyunguduka.Kana iyo dosi imwe chete iri diki, inokurudzirwa kuchengeta pa -20 ° C mushure mealiquoting.
3. Chengetedza zvikamu mushure mekuita pa 4 ° C, dzivisa kudzokorora kutonhora uye kunyunguduka, uye usagunzva reagents musati waita kuti urege kusvibiswa.
