SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Nzira)

SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Nzira)

Tsananguro yeKit:

Iyi kit inoshandisa Real-nguva RT PCR tekinoroji (rRT-PCR) yekuona kwakaringana kweSARS-CoV-2 (ORF1ab geni uye N gene) nucleic acids muvanhu nasopharyngeal kana oropharyngeal swab sampuli, nema multiplex PCR fluorescent probe nzira. kutonga kwemukati kuongorora mhando yemhando yepamusoro, nekudaro hapana chikonzero chekucheneswa kweaconic, inogona kupedzisa bvunzo muawa rimwe chete, kunyanya inokodzera kuona kukurumidza.


Chigadzirwa Chigadzirwa

Chigadzirwa Mateki

Magadzirirwo

48 Rxns, 96 Rxns

Aihwa.

Chikamu

Mari

Main zvinoriumba

48 Rxns

96 Rxns

1 Nucleic acid kuburitsa mumiriri 1.4mL / chubhu 2 machubhu 5.3 mL / bhodhoro 1 bhodhoro Surfactant
2 RNA inodzivirira 27 μL / chubhu 1 chubhu 53 μL / chubhu 1 chubhu RNase inhibitor
3 SARS-CoV-2 mhinduro mhinduro 800 μL / chubhu 1 chubhu 1600 μL / chubhu 1 chubhu Primer, probe, reaction buffer, dNTP
4 SARS-CoV-2 musanganiswa we enzyme 80 μL / chubhu 1 chubhu 160 μL / chubhu 1 chubhu Kutanga kupisa Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 yakanaka kutonga 100 μL / chubhu 1 chubhu 100 μL / chubhu 1 chubhu Recombinant plasmid ine chipenga chidimbu, RNA
6 SARS-CoV-2 isina kunaka kutonga 1200 μL / chubhu 1 chubhu 1200 μL / chubhu 1 chubhu TE buffer

Zvimiro & zvakanakira

Kurumidza uye nyore.
Dzivisa nucleic acid kurasikirwa.
Yakaderera yekuona muganho uye yakakwirira kunzwisisika
Yakaderera michina inodikanwa uye kushandiswa kwakanyanya kushanduka.

Kuziva kunzwisisika kunogona kusvika padanho repasi semakopi mazana / mL)

GCE / mL Ct kukosha
N geni ORF1ab geni
2.00E + 08 16.43 15.16
2.00E + 07 19.48 18.58
2.00E + 06 22.71 21.86
2.00E + 05 25.95 25.33
2.00E + 04 29.30 28.49
2.00E + 03 32.37 31.37
2.00E + 02 35.32 34.47
2.00E + 01 38.29 38.27
2.00E + 00 N / A. N / A.
2.00E-01 N / A. N / A.
NC N / A. N / A.

Linear amplification munyonga

amplification curve-RT-qPCR

Kushandisa sei

1. Chishandiso chinoshanda

covid-19 nucleic acid detection kit1

2. Maitiro ekutsvaga

covid-19 nucleic acid detection kit2

Shanduro yekugadzirisa: Naopharyngeal kana oropharyngeal swab sampuli (chete 1 nhanho mashandiro) (Mirira gumi mins)

PCR amplificaton (<= 55min)

Kufamba kwebasa:

covid-19 nucleic acid detection kit4

Kuchengetedza

chisimbiso kubva pachiedza uye chakachengetwa pa -20 ± 5 ℃;

storage
storage2

Pasherufu hupenyu: 1 gore


  • Pashure:
  • Zvadaro:

  • Nyora yako meseji pano uye uitumire kwatiri