SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)
Kit Description:
◮ Yakananga PCR:Hapana kudikanwa kweiyo nucleic acid yekubvisa kit uye System, yakapedzwa 96 specimen muawa imwe kana zvakadaro.
◮ Kudiwa kwemidziyo yakaderera uye kushandiswa kwakanyanya kuchinjika:Inongoda Real-Nguva PCR system.
◮ LoD uye yakanyanya kunzwa:Detection senitivity inogona kusvika padanho rakaderera se500 makopi/ml.
Chinangwa Kushandiswa
Iyi kit inoshandisa Real-time RT PCR tekinoroji (rRT-PCR) yemhando yekuona yeSARS-CoV-2 nucleic acids mumunhu nasopharyngeal kana oropharyngeal swab samples.
Nheyo yekuedza
Iyi yekutanga uye seti yekuferefeta yakagadzirirwa kucherechedzwa kweakachengetwa akateedzana eSARS-CoV-2 (ORF1ab gene uye N gene).Iyo kit ine Yemukati Kudzora iyo inoshandiswa kuongorora mhando yemuenzaniso.
Real-Nguva PCR tekinoroji inoshandisa polymerase chain reaction (PCR) kukwidziridza chinangwa chaicho.
kutevedzana uye kunongedza chaiwo maprobe ekuonekwa kweiyo RNA yakasimudzwa.Maprobes akanyorwa ne
fluorescent reporter uye quencher dhayi.
Quick Details
◮Zvinodiwa:48 Rxns/kiti, 96 Rxns/kiti
◮ Zvakananga:96.72%
Sensitivity:97.92%
◮Inoshanda mhando dzemuenzaniso
Nasopharyngeal Swab kana Oropharyngeal Swab
◮Chimbo chinoshanda
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Zvikamu
| Aihwa. | Chikamu | Mari | Main components | |||
| 48 Rxns | 96 Rxns | |||||
| 1 | Nucleic acid kuburitsa agent | 1.4mL/chubhu | 2 machubhu | 5.3 mL / bhodhoro | 1 bhodhoro | Surfactant |
| 2 | RNA inodzivirira | 27 μL/chubhu | 1 chubhu | 53 μL/chubhu | 1 chubhu | RNase inhibitor |
| 3 | SARS-CoV-2 reaction solution | 800 μL / chubhu | 1 chubhu | 1600 μL/chubhu | 1 chubhu | Primer, probe, reaction buffer, dNTP |
| 4 | SARS-CoV-2 enzyme musanganiswa | 80 μL/chubhu | 1 chubhu | 160 μL/chubhu | 1 chubhu | Kupisa kutanga Taq enzyme, M-MLV enzyme |
| 5 | SARS-CoV-2 kudzora kwakanaka | 100 μL / chubhu | 1 chubhu | 100 μL / chubhu | 1 chubhu | Recombinant plasmid ine chinangwa chidimbu, RNA |
| 6 | SARS-CoV-2 negative control | 1200 μL / chubhu | 1 chubhu | 1200 μL / chubhu | 1 chubhu | TE buffer |
Zvimiro&zvakanakira
◮Direct PCR
Hapana kudikanwa kweiyo nucleic acid yekubvisa kit uye System, yakapedzwa 96 specimen muawa imwe kana zvakadaro.
◮Kudiwa kwemidziyo yakaderera uye kushandiswa kwakanyanya kuchinjika
Inongoda Real-Nguva PCR system.
◮LoD uye yakanyanya kunzwa
Detection senitivity inogona kusvika padanho rakaderera se500 makopi/ml.
Kushandisa sei
Nzira 1: Yakananga PCR nzira
Nzira 2: Viral RNA Isolation + PCR
Nzira 1: Yakananga PCR nzira uchishandisaSample Release Agent
Nzira yekuona
Cold Chain Kutakura uye Kuchengeta
Yakavharwa kubva kuchiedza uye yakachengetwa pa -20±5 ℃;
Sherufu hupenyu
1 gore
Kuraira Ruzivo
Quick Operation vhidhiyo
Nyora meseji yako pano ugotitumira
