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SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)

Kit Description:

 

◮ Yakananga PCR:Hapana chikonzero cheiyo nucleic acid extaction kit uye System, yakapedza 96 specimen muawa imwe kana zvakadaro.

 

◮ Kudiwa kwemidziyo yakaderera uye kushandiswa kwakanyanya kuchinjika:Inongoda Real-Nguva PCR system.

 

◮ LoD uye yakanyanya kunzwa:Kuona senitivity kunogona kusvika padanho rakaderera se500 makopi/ml.

foregene simba


Product Detail

Product Tags

Chinangwa Kushandiswa

Iyi kit inoshandisa Real-time RT PCR tekinoroji (rRT-PCR) yemhando yekuona yeSARS-CoV-2 nucleic acids mumunhu nasopharyngeal kana oropharyngeal swab samples.

Nheyo yekuedza

Iyi yekutanga uye seti yekuferefeta yakagadzirirwa kuoneswa kweakachengetedzwa akatevedzana eSARS-CoV-2 (ORF1ab gene uye N gene).Iyo kit ine Yemukati Kudzora iyo inoshandiswa kuongorora mhando yemuenzaniso.
Real-Nguva PCR tekinoroji inoshandisa polymerase chain reaction (PCR) kukwidziridza chinangwa chaicho.
kutevedzana uye kunongedza chaiwo maprobe ekuonekwa kweiyo RNA yakasimudzwa.Maprobes akanyorwa ne
fluorescent reporter uye quencher dhayi.

Quick Details

Zvinodiwa:48 Rxns/kiti, 96 Rxns/kiti

◮ Zvakananga:96.72%

Sensitivity:97.92%

◮Inoshanda mhando dzemuenzaniso
Nasopharyngeal Swab kana Oropharyngeal Swab
Chimbo chinoshanda
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S

 

Zvikamu

Aihwa.

Chikamu

Mari

Main components

48 Rxns

96 Rxns

1 Nucleic acid kuburitsa agent 1.4mL/chubhu 2 machubhu 5.3 mL / bhodhoro 1 bhodhoro Surfactant
2 RNA inodzivirira 27 μL/chubhu 1 chubhu 53 μL/chubhu 1 chubhu RNase inhibitor
3 SARS-CoV-2 reaction solution 800 μL / chubhu 1 chubhu 1600 μL/chubhu 1 chubhu Primer, probe, reaction buffer, dNTP
4 SARS-CoV-2 enzyme musanganiswa 80 μL/chubhu 1 chubhu 160 μL/chubhu 1 chubhu Kupisa kutanga Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 kudzora kwakanaka 100 μL / chubhu 1 chubhu 100 μL / chubhu 1 chubhu Recombinant plasmid ine chinangwa chidimbu, RNA
6 SARS-CoV-2 negative control 1200 μL / chubhu 1 chubhu 1200 μL / chubhu 1 chubhu TE buffer

Zvimiro&zvakanakira

Direct PCR
Hapana chikonzero cheiyo nucleic acid extaction kit uye System, yakapedza 96 specimen muawa imwe kana zvakadaro.
Kudiwa kwemidziyo yakaderera uye kushandiswa kwakanyanya kuchinjika
Inongoda Real-Nguva PCR system.
LoD uye yakanyanya kunzwa
Kuona senitivity kunogona kusvika padanho rakaderera se500 makopi/ml.
amplification curve-RT-qPCR

Kushandisa sei

Nzira 1: Yakananga PCR nzira

Nzira 2: Viral RNA Isolation + PCR

Nzira 1: Yakananga PCR nzira uchishandisaSample Release Agent  

 

Nzira yekuona

Maitiro Okushandisa

Maitiro

Cold Chain Kutakura uye Kuchengeta

Yakavharwa kubva kuchiedza uye yakachengetwa pa -20±5 ℃;

storage
kuchengetedza2

Sherufu hupenyu

1 gore

Kuraira Ruzivo

Kuraira ruzivo

Quick Operation vhidhiyo


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