Gel Extraction Kit DNA Gel Extraction Kit
Kit Description:
Kurumidza uye nemazvo dzora 20bp-10kb DNA zvimedu kubva kune agarose gel.
◮Yakasiyana-siyana yeDNA kupora:Zvimedu zveDNA zvakapfupika se30bp uye zvakakura se10kb zvinogona kudzoserwa.
◮High kupora kushanda zvakanaka:Iyo yakanyanya kudzoreredza inoshanda inogona kusvika kupfuura 80%.
◮Small system elution:kanenge 30μl elution mhinduro inogona kushandiswa elution, iyo inogona zvinobudirira kuwedzera kuunganidzwa kwezvakadzoreredzwa zvimedu zveDNA.
◮KumhanyaZviri nyore kushanda, DNA fragment kudzoreredza inogona kupedzwa mukati memaminitsi gumi nemashanu.
◮Safety:Hapana organic reagent kutorwa kunodiwa.
◮Hunhu hwepamusoro:Zvimedu zveDNA zvakadzoserwa ndezvekuchena kwepamusoro, izvo zvinogona kusangana nemiedzo yakasiyana-siyana inotevera.
Tsanangudzo
Iyi kit inoshandisa spin column uye fomula yakagadziriswa nekambani yedu, iyo inogona kunyatso kudzoreredza yakakwirira-kuchena zvidimbu zveDNA kubva kuagarose gel.Iyo kit inogona kudzoreredza zvimedu zveDNA munzvimbo dzakasiyana.Mumamiriro ezvinhu akajairwa, zvidimbu zveDNA zve30bp-10kb zvinogona kudzoserwa.Iyo shoma ye30μl yeeluent inogona kushandiswa kuwedzera kuunganidzwa kweDNA yakadzoserwa.
Iyo kit iri nyore kushandisa uye ine matanho mashoma.Inongoda akati wandei centrifugation kugadzirisa akawanda masampuli panguva imwe chete, uye yakakwirira-kuchena yakadzoreredzwa zvidimbu zveDNA zvinogona kuwanikwa mumaminetsi gumi nemashanu.
Zvinotsanangurwa
50 Preps
Buffer GE: Dissolve the gel block pa60 ° C kuburitsa zvidimbu zveDNA kubva mujeli.
Buffer WB1: Bvisa maion emunyu asara muDNA.
Buffer EB: bvisa iyo DNA pane yekuchenesa column membrane.
DNA-Chete Column: Yakananga adsorption yeDNA zvimedu mugel.
Kit zvikamu
| Buffer GE* |
| Buffer WB1 |
| Buffer EB |
| DNA-Chete Column |
| Mirayiridzo |
Zvimiro&zvakanakira
-Kuwanda kweDNA kupora: Zvimedu zveDNA zvakapfupika se30bp uye zvakakura se10kb zvinogona kudzoserwa.
-Kunyanya kudzoreredza kushanda zvakanaka: Iyo yakanyanya kudzoreredza kugona inogona kusvika kupfuura 80%.
-Small system elution: kanenge 30μl elution mhinduro inogona kushandiswa kujekesa, iyo inogona zvinobudirira kuwedzera kuwanda kweakadzoreredzwa zvidimbu zveDNA.
-Kukurumidza kumhanya: Zviri nyore kushanda, DNA chidimbu kudzoreredza inogona kupedzwa mukati memaminitsi gumi nemashanu.
-Kuchengeteka: Hapana organic reagent kutorwa kunodiwa.
-Hunhu hwepamusoro: Zvimedu zvakadzoserwa zveDNA ndezvekuchena kwepamusoro, izvo zvinogona kusangana nemiedzo yakasiyana-siyana inotevera.
Kit application
Kiti iyi yakakodzera kudzoreredza zvimedu zveDNA (30bp-10kb) muagarose gels.
Workflow
Storage uye Sherufu upenyu
Kiti iyi inogona kuchengetwa kwemwedzi gumi nemiviri pasi pekuoma kwemamiriro ekunze (15-25 ° C), kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8 ° C.
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.
