Ropa SuperDirectᵀᴹ PCR Kit-EDTA Ropa Rakananga PCR Master Mix yeGenotyping yeRopa.
Kit Description:
Shandisa zvakananga EDTA anticoagulated ropa rose se template yePCR amplification, ine high sensitivity uye yakakwirira amplification kunyatsoshanda.
◮Hapana chikonzero chekutora nguva uye inodhura DNA kunatswa, hapana pretreatment, uye zvakananga kushandisa ropa rose sePCR template.
◮Iyo sisitimu ine simba rekusimudzira uye inogona kunyatsoona genome uye yekunze inonangwa zvidimbu zveDNA muropa.
◮2 × SuperEasyTMSanganisa-EDTA Kushivirira kweropa kwepamusoro: kusvika ku45% muropa revanhu uye 20% muropa rembeva.
◮Sample yakavharirwa zvizere, saka hapana chikonzero chekunetseka nezvekusvibiswa kwemuenzaniso uye nhema dzakanaka dzePCR.
Zvinotsanangurwa
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMMix-EDTA: Iine yakagadziridzwa D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer uye stabilizer, nezvimwewo. Mukuita kwePCR, ingo wedzera iro rakakodzera anticoagulated ropa rose, primers, uye ddH.2O kune inoenderana 2 × SuperEasyTMMix-EDTA inoshandiswa muPCR reaction.
6 × DNA Loading Buffer: Iyo Loading Buffer haina SDS.Zvinokurudzirwa kushandisa 6 × DNA Loading Buffer inosanganisirwa nekiti paunenge uchiita agarose gel electrophoresis kuitira kuti uwane yakanaka electrophoresis mhinduro.Usashandise Loading Buffer ine SDS, zvikasadaro pachava nemuswe wakakura wechiedza chakajeka munzira panguva ye electrophoresis, izvo zvinokanganisa mhedzisiro yekuedza.
Kit zvikamu
| 2 × SuperEasyTMMix-EDTA |
| 6 × DNA Loading Buffer |
| Mirayiridzo |
Zvimiro&zvakanakira
-Hapana chikonzero chekutsvaga nguva uye inodhura DNA kuchenesa, kwete pretreatment, uye kushandiswa zvakananga kweropa rose sePCR template.
-Iyo sisitimu ine simba rekusimudzira uye inogona kunyatsoona iyo genome uye yekune dzimwe nyika chinangwa cheDNA zvimedu muropa.
-2 × SuperEasyTMSanganisa-EDTA Kushivirira kweropa kwepamusoro: kusvika ku45% muropa revanhu uye 20% muropa rembeva.
-Sample yakavharirwa zvizere, saka hapana chikonzero chekunetseka nezvekusvibiswa kwemuenzaniso uye nhema dzakanaka dzePCR.
Kit application
Yakatsaurirwa kutungamira PCR kuzivikanwa kweEDTA anticoagulated ropa rose.(Kusanganisira: ropa remunhu, ropa rembeva, ropa rehuku, ropa reshiri, ropa remombe, ropa rembwa, nezvimwewo)
Workflow
Storage uye Sherufu upenyu
Iyi kit inofanira kuchengetwa pa -20 ° C.
2 × SuperEasyTMMix-EDTA, kana yakashandiswa kakawanda, inogonawo kuchengetwa pa4 ℃ kuitira kuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi).
6 × DNA Loading Buffer, inogona kuchengetwa pa 4 ° C kana -20 ° C kwenguva yakareba.
