Mhuka miRNA Isolation Kit MicroRNA Kudhonza uye Kuchenesa Kits yeMhuka
Kit Description:
Kurumidza uye nekunyatso bvisa zvimedu zvidiki zveRNA zve20-200nt miRNA, siRNA, snRNA kubva kune akasiyana matishu emhuka uye maseru.
◆Iyo yese process inoshandiswa pakudziya kwekamuri (15-25°C), pasina kugeza kwechando uye kutsika-kupisa centrifugation.
◆Yakazara set kit RNase-Yemahara, hapana chikonzero chekunetseka nezveRNA kudzikira.
◆DNA-Kuchenesa Column inosunga chaizvo DNA, kuitira kuti kit inogona kubvisa genomic DNA kusvibiswa pasina kuwedzera imwe DNase.
◆Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatso chenesa RNA.
◆Kukurumidza kumhanya: Kushanda kuri nyore uye kunogona kupedzwa mukati memaminitsi makumi matatu.
◆Chengetedzo: hapana organic reagent kutorwa kunodiwa.
◆Hunhu hwepamusoro: Zvimedu zveRNA zvakabviswa ndezvekuchena kwepamusoro, zvisina mapuroteni uye kumwe kusvibiswa kwetsvina, uye zvinogona kusangana nezvinodiwa zvekudzika kwakadzika.
◆Various experimental applications
Tsanangudzo
Iyi kit inoshandisa spin column uye fomula yakagadziridzwa nekambani yedu, iyo inogona kunyatsoburitsa zvimedu zvidiki zveRNA senge 20-200nt miRNA, siRNA, snRNA kubva kune akasiyana matishu emhuka nemaseru.Iyo kit inopa DNA-Cleaning Column, iyo inogona nyore kuparadzanisa supernatant kubva munyama lysate uye kubvisa DNA uye refu RNA kubva sampuli, iyo iri nyore uye inochengetedza nguva;RNA-chete Column inokwanisa kusunga RNA zvine mafomula akasiyana, Inogona kugadzirisa nhamba yakakura yemasampuli panguva imwe chete.Iyo yese sisitimu ndeye RNase-Yemahara, kuitira kuti iyo yakacheneswa miRNA irege kuderedzwa;Buffer miRW1, Buffer miRW2 buffer washing system, kuitira kuti iyo RNA inowanikwa haina protein, DNA, ions, uye organic compound kusvibiswa.
Kit zvikamu
| Mhuka miRNA Isolation Kit | ||||
| Kit zviri mukati | RE-0101T | RE-01011 | RE-01012 | RE-01014 |
| 5 T | 50 T | 100T | 200T | |
| Buffer miRL* | 3ml | 25ml | 50ml | 100ml |
| Buffer miRW1 | 4ml | 35ml | 70ml | 140ml |
| Buffer miRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-Yemahara ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| RNA-chete Column | 5 sets | 50sets | 100sets | 200sets |
| DNA-Kuchenesa Column | 5 sets | 50sets | 100sets | 200sets |
| IFU | 1 chidimbu | 1 chidimbu | 1 chidimbu | 1 chidimbu |
Zvimiro&zvakanakira
◆Iyo yese process inoshandiswa pakudziya kwekamuri (15-25°C), pasina kugeza kwechando uye kutsika-kupisa centrifugation.
◆Yakazara set kit RNase-Yemahara, hapana chikonzero chekunetseka nezveRNA kudzikira.
◆DNA-Kuchenesa Column inosunga chaizvo DNA, kuitira kuti kit inogona kubvisa genomic DNA kusvibiswa pasina kuwedzera imwe DNase.
◆Yakakwira RNA goho: RNA-chete Column uye yakasarudzika fomula inogona kunyatso chenesa RNA.
◆Kukurumidza kumhanya: Kushanda kuri nyore uye kunogona kupedzwa mukati memaminitsi makumi matatu.
◆Chengetedzo: hapana organic reagent kutorwa kunodiwa.
◆Hunhu hwepamusoro: Zvimedu zveRNA zvakabviswa ndezvekuchena kwepamusoro, zvisina mapuroteni uye kumwe kusvibiswa kwetsvina, uye zvinogona kusangana nezvinodiwa zvekudzika kwakadzika.
◆Various experimental applications
Kit application
Inokodzera kubviswa uye kucheneswa kwe miRNA kubva kumhando dzakasiyana dzemhuka nyowani kana chando tishu kana maseru.
Storage uye Sherufu upenyu
Iyi kit inogona kuchengetwa kwemwedzi makumi maviri nemana pasi pemamiriro akaoma pane tembiricha yekamuri (15-25°C), kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8°C.Buffer miRL inogona kuiswa pa4°Ckwemwedzi 1 mushure mekuwedzeraβ-mercaptoethanol(zvinokurudzirwa kuwedzera izvozvi).
