Ropa DNA Mini Kit
Kit Description:
Kurumidza kuchenesa yemhando yepamusoro genomic DNA kubva kune anticoagulated ropa (<1ml).
◮Hapana RNase kusvibiswa:DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva mujenomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
◮KumhanyaForegene Protease ine chiitiko chepamusoro kupfuura maprotease akafanana, uye inogaya masampula enyama nekukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me20-80 maminetsi.
◮Yakanakira:Iyo centrifugation inoitwa pakamuri tembiricha, uye hapana chikonzero che4 ° C yakaderera-tembiricha centrifugation kana ethanol kunaya kweDNA.
◮Safety:Hapana organic reagent extract inodiwa.
◮Hunhu hwepamusoro:Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
◮Micro-elution system:Inogona kuwedzera kuwanda kweiyo genomic DNA, iyo yakanakira kudzika kwerwizi kana kuyedza.
Kit Descriptions
Iyi kit inotora itsva Foregene Protease Plus uye yakasarudzika BL1 uye BL2 buffer masisitimu, anogona kugaya zvachose anticoagulant masampu eropa munguva pfupi, nekudaro kudzivirira kuderedzwa kweDNA kusvika padanho repamusoro uye kuwana huwandu hwepamusoro hwegenomic DNA.Iyo yekukurumidza kugadzirisa ropa system uye iri nyore DNA-Chete column operation inorerutsa zvakanyanya kutorwa kweropa genome, kuita iyo genomic DNA inogona kuwanikwa yemhando yepamusoro uye kuchena kwepamusoro mukati memaminitsi makumi mana.
Iyo DNA-chete silica gel membrane inoshandiswa mumutsara wecentrifuge wekiti iyi rudzi rutsva rwezvinhu zvakasiyana nekambani yedu, iyo inogona kusunga DNA zvakanyatsonaka uye zvakananga, uye inogona kubvisa RNA, tsvina, mapuroteni, ions uye mamwe organic compounds mumasero kusvika pamwero mukuru.Izvo zvakawanikwa zvidimbu zveDNA zvakakura, kuchena kwepamusoro, kugadzikana uye kuvimbika kwemhando.Hukuru hwekutakura huwandu hweDNA-Chete column ndeye 80 μg DNA.Iyo yakawanikwa DNA inogona kushandiswa mumamolecular biology kuyedza senge enzyme digestion, PCR, Southern hybridization uye kuvaka raibhurari.
Iyo kit inogona kugadzira kusvika ku1ml yeropa panguva kuti iwane huwandu hwakakwana hwepamusoro-kuchena genomic DNA.
Zvinotsanangurwa
| Ropa DNA Mini Kit | |||
| Kit zviri mukati | DE-05111 | DE-05112 | DE-05113 |
| 50 T | 100 T | 250 T | |
| Buffer BL1 | 15 mL | 30 ml | 75 ml |
| Buffer BL2 * | 15 mL | 30 ml | 75 ml |
| Buffer DC | 100 mL | 200 ml | 500 ml |
| Buffer PW * | 25 mL | 50 ml | 125 ml |
| Buffer WB1 | 15 mL | 30 ml | 75 ml |
| Buffer EB | 10 mL | 20 ml | 50 ml |
| Foregene Protease Plus | 1.25 mL | 2.5 ml | 6.5 ml |
| DNA-Chete Column | 50 | 100 | 250 |
| Manual | 1 | 1 | 1 |
*: Buffer BL2 uye Buffer PW ine inovava mvura yemunyu.Ndokumbira upfeke magirovhosi uye tora matanho ekudzivirira akakodzera paunenge uchishanda.
Zvimiro&zvakanakira
-Kwete kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva kune genomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
-Kukurumidza kukurumidza: Foregene Protease Plus ine basa rakakwirira kudarika maproteases akafanana, uye inochera sampuli dzenyama nokukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me40 maminetsi.
-Kusununguka: Iyo centrifugation inoitwa pakupisa kwekamuri, 4 ° C centrifugation kana ethanol precipitation yeDNA haidiwi.
-Kuchengeteka: Hapana organic reagent kutorwa kunoshandiswa.
-Hunhu hwepamusoro: Iyo yakabviswa genomic DNA zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, iyo inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
Kit application
Inokodzera kubviswa uye kucheneswa kwegenomic DNA kubva kutsva kana chando anticoagulated ropa rose.
Kuyerera kwebasa
Kuchengetedza uye hupenyu hwesherufu
Iyi kit inogona kuchengetwa kwemwedzi gumi nemiviri pasi pemamiriro akaoma panzvimbo yekupisa (15-25 ° C);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8°C.
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.
Iyo Foregene Protease Plus mhinduro ine yakasarudzika formula, inoshanda kana yakachengetwa mukamuri tembiricha kwenguva yakareba (3 mwedzi);ichave yakanyanya kushanda uye yakagadzikana kana yakachengetwa pa4 ° C, saka inokurudzirwa kuichengeta pa4 ° C, yeuka kuti usaichengeta pa -20 ° C.
