Stool DNA Isolation Kit DNA Extraction Purification Kits kubva Stool
Kit Description:
Kurumidza kuchenesa uye uwane yemhando yepamusoro genomic DNA kubva kune akasiyana stool samples.
◮Hapana RNase kusvibiswa:DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva mujenomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
◮KumhanyaForegene Protease ine chiitiko chepamusoro kupfuura maprotease akafanana, uye inogaya masampula enyama nekukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me20-80 maminetsi.
◮Yakanakira:Iyo centrifugation inoitwa pakamuri tembiricha, uye hapana chikonzero che4 ° C yakaderera-tembiricha centrifugation kana ethanol kunaya kweDNA.
◮Safety:Hapana organic reagent extract inodiwa.
◮Hunhu hwepamusoro:Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
◮Micro-elution system:Inogona kuwedzera kuwanda kweiyo genomic DNA, iyo yakanakira kudzika kwerwizi kana kuyedza.
Tsanangudzo
Iyi kit inopa nzira inokurumidza uye iri nyore yekutora genomic DNA kubva kwakasiyana siyana kwevhu.Kune huwandu hukuru hwezvidziviriro zvakaita sehumic acid nesimbi ions mumasampuli evhu.Kunyangwe kana zvinhu izvi zviripo mune zvidiki muDNA yakacheneswa, zvichave nemhedzisiro pakuita kwepasi, sePCR uye kurambidza kugaya enzyme.Naizvozvo, kiyi yekuchenesa ivhu DNA inzira yekunyatso bvisa inhibitors muvhu.
Kiti iyi inoshandisa DNA-Chete Column inogona kunyatsosunga kuDNA, nyowani-itsva Foregene Protease uye yakasarudzika buffer system, inogona kunyatso bvisa zvakasiyana inhibitors kubva muvhu pasina organic solvent extraction kana ethanol uye isopropanol kunaya.Kubviswa kweDNA kubva kune ivhu samples kunogona kupedzwa mumaminitsi makumi mapfumbamwe.
Zvinotsanangurwa
25 Preps, 50 Preps, 100 Preps, 250 Preps
Kit zvikamu
| Buffer SL1 |
| Buffer SL2 |
| Buffer SL3 |
| Buffer PW |
| Buffer WB |
| Buffer EB |
| Buffer TE |
| Foregene Protease |
| Lysozyme |
| DNA-Chete Column |
| Mirayiridzo |
Zvimiro&zvakanakira
-Hapana kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva mujenomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira marabhoritari kuti isasvibiswa neRNase yekunze.
-Kukurumidza kumhanya: Kushanda kuri nyore, uye iyo fecal genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me40 maminetsi.
-Yakanaka: Iyo centrifugation inoitwa pane tembiricha yekamuri, uye hapana chikonzero che4 ° C yakaderera-kupisa centrifugation kana ethanol precipitation yeDNA.
-Kuchengeteka: Hapana organic reagent kutorwa kunodiwa.
-Hunhu hwepamusoro: Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
Kit application
Yakakodzera kucheneswa kwegenomic DNA kubva kune anotevera masampuli: munhu mutsva kana akaomeswa nechando, mhou, huku, mbeva uye mamwe masampuli endove.
Workflow
Storage uye Sherufu upenyu
-Iyi kititi inogona kuchengetwa kwemwedzi gumi nemaviri pasi pemamiriro akaoma pakushambidzika kwekamuri (15-25 ° C);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8°C.
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.
-Forgene Protease solution ine chimiro chakasiyana-siyana, iyo inoshanda kana yakachengetwa mukamuri yekushisa kwenguva yakareba (mwedzi mitatu), basa rayo uye kugadzikana zvichava nani kana zvakachengetwa pa4 ° C, saka zvinokurudzirwa kuichengeta pa 4 ° C, yeuka kuti usaichengeta pa -20 ° C.
-Dry Lysozyme powder inochengetwa pa -20 ° C, mhinduro yeLysozyme yakagadzirirwa inoparadzaniswa kuita zvikamu zviduku uye inochengetwa pa -20 ° C.
