PCR Yekuchenesa Kit PCR Yekuchenesa-System YeUltra-pure DNA
Kit Description:
Kurumidza kuchenesa uye uwane zvemhando yepamusoro zvidimbu zveDNA kubva kuPCR system;kana iwe uchida kupatsanura uye kuwana chaiwo zvidimbu zveDNA, ndapota sarudza kit yekudzoreredza gel.
◮ Yakasiyana-siyana yeDNA kupora: Zvimedu zveDNA zvakapfupika se60bp uye zvakakura se10kb zvinogona kudzoserwa.
◮ Kudzora kwakanyanya: Kudzoreredza kunyatsoita kunowanzo kupfuura 80%, uye iyo yepamusoro inogona kusvika kupfuura 95%.
◮ Kukurumidza kumhanya:Zviri nyore kushanda, DNA chidimbu kudzoreredza inogona kupedzwa mukati memaminitsi gumi nemashanu uye primer dimer inogona kubviswa zvakananga pasina kudzoreredza gel.
◮ Safety:Hapana organic reagent kutorwa kunodiwa.
◮ Hunhu hwepamusoro: Zvimedu zveDNA zvakadzoserwa ndezvekuchena kwepamusoro, izvo zvinogona kusangana nemiedzo yakasiyana-siyana inotevera.
Tsanangudzo
Iyi kit inoshandisa spin column uye fomula yakagadziriswa nekambani yedu, iyo inogona kunyatso kudzoreredza yakakwira-kuchena zvidimbu zveDNA kubva kuPCR system.Iyo mbiru yekuchenesa inogona kunyatso kusunga DNA, uye inofananidzwa neyakasarudzika formula reagent kuti ibvise zvakananga primer dimer pasina kudzoreredza gel.
Iyo kit inotora huwandu hwakawanda hwezvimedu zveDNA.Mumamiriro ezvinhu akajairwa, zvidimbu zveDNA zvakapfupika se60bp zvinogona kudzoserwa.Iyo shoma ye30μl yeeluent inogona kushandiswa kuwedzera kuunganidzwa kweDNA yakadzoserwa.
Iyo kit iri nyore kushandisa uye ine matanho mashoma.Inongoda akati wandei centrifugation kugadzirisa akawanda masampuli panguva imwe chete, uye yakakwirira-kuchena yakadzoreredzwa zvidimbu zveDNA zvinogona kuwanikwa mumaminetsi gumi nemashanu.
Zvinotsanangurwa
50 Preps, 100Preps, 250Preps
Kit zvikamu
| Buffer BD* |
| Buffer BD-S |
| Buffer WB1 |
| Buffer EB |
| DNA-Chete Column |
| Mirayiridzo |
Zvimiro&zvakanakira
-Kuwanda kweDNA kupora: Zvimedu zveDNA zvakapfupika se60bp uye zvakakura se10kb zvinogona kudzoserwa.
-Kunyanya kudzoreredza kushanda zvakanaka: Kudzoreredza kushanda zvakanaka kunowanzo kupfuura 80%, uye iyo yepamusoro inogona kusvika kupfuura 95%.
-Kukurumidza kumhanya: Zviri nyore kushanda, DNA chidimbu kudzoreredza inogona kupedzwa mukati memaminitsi gumi nemashanu uye primer dimer inogona kubviswa zvakananga pasina kupora gel.
-Kuchengeteka: Hapana organic reagent kutorwa kunodiwa.
-Hunhu hwepamusoro: Zvimedu zvakadzoserwa zveDNA ndezvekuchena kwepamusoro, izvo zvinogona kusangana nemiedzo yakasiyana-siyana inotevera.
Kit application
Iyi kit yakakodzera kudzoreredza zvidimbu zveDNA (60bp-10kb) muPCR zvigadzirwa.
Workflow
Storage uye Sherufu upenyu
Iyi kit inogona kuchengetwa kwemwedzi gumi nemiviri pasi pemamiriro akaoma panzvimbo yekupisa (15-25 ° C);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8°C.
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.
