Mvura DNA Isolation Kit DNA Kutora uye Kuchenesa Kit yeMvura
Kit Description:
Kurumidza kuchenesa yemhando yepamusoro genomic DNA kubva kune tupukanana mumhando dzakasiyana dzemvura samples.
◮Hapana RNase kusvibiswa:DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva mujenomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
◮KumhanyaForegene Protease ine chiitiko chepamusoro kupfuura maprotease akafanana, uye inogaya masampula enyama nekukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me20-80 maminetsi.
◮Yakanakira:Iyo centrifugation inoitwa pakamuri tembiricha, uye hapana chikonzero che4 ° C yakaderera-tembiricha centrifugation kana ethanol kunaya kweDNA.
◮Safety:Hapana organic reagent extract inodiwa.
◮Hunhu hwepamusoro:Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
◮Micro-elution system:Inogona kuwedzera kuwanda kweiyo genomic DNA, iyo yakanakira kudzika kwerwizi kana kuyedza.
Zvinotsanangurwa
50 Preps
-Lysozyme: enzymatic hydrolyze sero rusvingo rwebhakitiriya rakanaka.
-Buffer TE: inoshandiswa kugadzirira 100mg / ml Lysozyme mhinduro uye inopa lysozyme enzymatic hydrolysis environment.
-Buffer SG1 & Buffer SG2: Ipa muenzaniso weprotease digestion nharaunda.
-Forgene Protease: Enzymatically hydrolyze samples munzvimbo yeprotease enzymatic kuburitsa genomic DNA.
-Buffer SG3: Ita kuti Foregene Protease uye ipe DNA yekurodha nharaunda.
-Buffer SG4: Supplement yekupa DNA yekurodha nharaunda.
-Buffer PW: Bvisa tsvina yakaita seprotein uye RNA muDNA.
-Buffer WB: Bvisa maion emunyu asara muDNA.
-Buffer EB: bvisa iyo DNA pane yekuchenesa column membrane.
-DNA-chete Column: kunyanya adsorb iyo genomic DNA mune lysate.
Kit zvikamu
| Buffer SG1 |
| Buffer SG2 |
| Buffer SG3 |
| Buffer SG4 |
| Buffer PW |
| Buffer WB |
| Buffer EB |
| Buffer TE |
| Foregene Protease |
| Lysozyme |
| DNA-Chete Column |
| Mirayiridzo |
Zvimiro&zvakanakira
-Hapana kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva kune genomic DNA pasina imwe RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neiyo exogenous RNase.
-Kukurumidza kumhanya: Kushanda kuri nyore, uye mvura genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me40 maminetsi.
-Yakanaka: Iyo centrifugation inoitwa pane tembiricha yekamuri, uye hapana chikonzero che4 ° C yakaderera-kupisa centrifugation kana ethanol precipitation yeDNA.
-Kuchengeteka: hapana chinodiwa organic reagent kudhirowa.
-Hunhu hwepamusoro: Iyo yakabviswa genomic DNA zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, iyo inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
Kit application
Inokodzera kucheneswa kweiyo genomic DNA yemasampuli anotevera: aquaculture pond pond, pombi mvura, mvura yemugungwa, mvura yerwizi, lotus pond pond uye mamwe masampuli.
Workflow
Storage uye Sherufu upenyu
-Iyi kit inogona kuchengetwa kwemwedzi gumi nemaviri pasi pemamiriro akaoma pamhepo yekushisa (15-25 ° C), kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa 2-8 ° C.
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat iyo mu37°C mvura yekugeza kwemaminetsi gumi kuti iparadze iyo precipitate, uye sanganisa isati yashandiswa.
-Forgene Protease mhinduro ine chimiro chakasiyana-siyana, iyo inoshanda kana yakachengetwa mukamuri yekushisa kwenguva yakareba (mwedzi mitatu);kana yakachengetwa pa4 ℃, basa rayo uye kugadzikana kuchava nani, saka zvinokurudzirwa kuichengeta pa4 ℃, yeuka kuti usaichengeta pa -20 ℃.
-Dry powder Lysozyme inochengetwa pa -20 ° C;iyo yakagadzirwa Lysozyme solution inokamurwa kuita zvidimbu zvidiki uye inochengetwa pa -20 ° C.
