Dyara Mbeu(Yakakura) Yakananga PCR Kit II-UNG(isina Sampling Tools)
Kit Description:
Kushandiswa kwakananga kwembeu lysate se template yePCR amplification, hapana yakaparadzana kutorwa kweDNA, nekukurumidza uye kwakanyanya kunzwisiswa.Iyo UNG anti-pollution system yakawedzerwa kudzivirira maitiro enhema.
◮Hapana nguva-inopedza uye inodhura yekuchenesa DNA inodiwa.
◮Sample inodiwa idiki, ingotora mhodzi imwe chete.
◮Hapana mishonga yakakosha yakadai sekugaya nekupwanya inodiwa, uye kushanda kuri nyore.
◮Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.
Tsanangudzo
Ichi chigadzirwa chinoshandisa yakasarudzika lysis reaction system, iyo inogona kukurumidza kuburitsa genomic DNA kubva kumiti yembeu samples ine yakaderera polysaccharide uye polyphenol content (senge: mupunga, gorosi, fodya, chibage, soya beans, etc.) kuti ishandiswe muPCR reactions, saka inonyanya kukodzera Makuru-scale genetic test.Kuti zvizadzise zvidikanwi zveongororo dzakasiyana-siyana, kit iyi inogona kushandisa mhando dzakawanda dzesampuli (sembeu dzizere, kucheka matishu madiki, kana sampuli dzepasi dzemhodzi dzakawanda) pakuedza.Pakati pazvo, kune sampuli dzembeu dzichiri kuda kumera, unogona kusarudza kucheka madiki-tishu (1-5mg) kunze kwembeu embryo yekuyedza;kune zviedzo zvinoda kutorwa muhuwandu hukuru hwemasamples, unogona kusarudza kusanganisa akawanda masampuli uye woasanganisa.Mushure mekugaya kuita zvidimbu zvine dhayamita ingangoita 0.5mm, kuyedza kunogona kuitwa (inenge 0.1% yeiyo inotarirwa sampu inogona kuonekwa).
Zvinotsanangurwa
50T,200T,500T,2000T
Kit zvikamu
| Dyara Mbeu Yakananga PCR Kit II-UNG Dyara Mbeu (Yakakura) Yakananga PCR Kit-UNG | |||||
| Kit Composition | TP-0314T | TP-03141 | TP-03142 | TP-03143 | |
| 50 T | 200T | 500T | 2000T | ||
| Chikamu I | Buffer SP1 | 30ml | 120ml | 100ml × 3 | 400ml × 3 |
| Buffer SP2 | 3ml | 10ml | 25ml | 100ml | |
| 6 × DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml × 4 | |
| Chikamu II | 2× Mbeu PCR NyoreTMMusanganiswa(UNG) | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml × 12 |
| Manual | 1 | 1 | 1 | 1 | |
Zvimiro uye zvakanakira
- Hapana chinodiwa chinopedza nguva uye chinodhura chekuchenesa DNA chinodiwa.
- Sample inodiwa idiki, ingotora mhodzi imwe chete.
- Hapana kurapa kwakakosha senge kukuya nekupwanya kunodiwa, uye kushanda kuri nyore.
- Iyo yakagadziridzwa PCR sisitimu inogonesa PCR kuti ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors.
- Anti-kusvibisa PCR system 2 × Mbeu PCR NyoreTMMix (UNG) inogona kunyatsobvisa kusvibiswa kunokonzerwa nePCR zvigadzirwa uye kuve nechokwadi cheiyo chaiyo uye nekururama kweamplification.
Workflow
Kuchengetedza mamiriro
1.Kutakurwa
Iyo yese maitiro ekudzika-tembiricha echando bhokisi chekufambisa, kuve nechokwadi chekuti kit Chikamu II chiri mumamiriro e <4 ℃.
2.Kuchengeta
Chikamu I chekiti ichi chinofanira kuchengetwa mukamuri tembiricha kana 2-8 ° C.
Chikamu II chinofanira kuchengetwa pa -20°C.
