EndoFree Maxi Plasmid Kit (Spin Column)
Kit Description:
Iyo yese yekubvisa maitiro inotora chete awa imwe, kuve nechokwadi chekushanda uye nekukurumidza kushanda.
Tsanangudzo
Iyo EndoFree Maxi Plasmid Kit inotora yakasarudzika silica membrane adsorption tekinoroji kuti inyatso sunga plasmid DNA.Nekubatanidza iyo yakakosha Endotoxin Removal Buffer P4 uye EndoFree Maxi Filtration Columns, endotoxin, mapuroteni uye kumwe kusvibiswa kunogona kubviswa zvinobudirira.Iyo yese yekubvisa maitiro inotora chete awa imwe, kuve nechokwadi chekushanda uye nekukurumidza kushanda.
Huwandu hwemabhakitiriya anokurudzirwa pakuita kwega kwega: Kune yakakwira-kopi plasmid, zvinokurudzirwa kushandisa 100 ml yebhakitiriya tsika media, uye goho rinowanzosvika 500-1500 μg.Kune yakaderera-kopi plasmid, 200 ml mabhakitiriya tsika midhiya inokurudzirwa kugadzira yakatenderedza 200-600 μg plasmids.
Kit zvikamu
| Product Zvikamu | D909-E(10 preps) |
| Buffer BL | 30 ml |
| Buffer P1 | 100 ml |
| Buffer P2 | 100 ml |
| Buffer P4 | 100 ml |
| Buffer DW2 | 44 ml |
| Buffer EB | 30 ml |
| RNase A(10 mg/ml) | 1 ml |
| DNAPure Maxi Spin Columns | 10 pcs |
| FinePure Maxi Sefa sirinji | 10 pcs |
| 50 ml Collection Machubhu | 10 pcs |
Kuonekwa kwePlasmid DNA Concentration uye Kuchena
Kuchena uye kusungirirwa kweiyo yakabviswa plasmid DNA inogona kuonekwa neagarose gel electrophoresis uye ultraviolet spectrophotometer.Ukoshi hweOD260 hwe1 hunoenderana neinosvika makumi mashanu μg/ml yeDNA yakasungwa kaviri.Iyo OD260/OD280 kukosha kweiyo yakacheneswa plasmid DNA inowanzova muhuwandu hwe1.7-1.9.Iyo yakacheneswa plasmid DNA inogona kushandiswa zvakananga kune zviedzo zvine kuchena kwakanyanya zvinodiwa, sekutapurirana sero kana mu vivo kuyedza.
Kit application
Iyo plasmid DNA yakabviswa nekiti iyi inogona kuiswa kune akasiyana maitiro ekuita, kusanganisira enzymatic digestion, PCR, kutevedzana, ligation, shanduko pamwe nekuchinjisa kune akasiyana siyana marudzi.
Storage uye Sherufu upenyu
EndoFree Maxi Plasmid Kit inogona kuchengetwa mukamuri tembiricha (15-25 ° C) pasi pemamiriro akaoma kusvika kumwedzi gumi nemaviri.Wedzera RNase A kuBuffer P1 usati watanga kushandisa, sanganisa zvakanaka wochengeta pa2-8°C.Musanganiswa unogona kuchengetwa zvakagadzikana kweanopfuura mwedzi mitanhatu.
