General Plasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kits
Kit Description:
Kurumidza kuchenesa yemhando yepamusoro plasmid DNA kubva kubhakitiriya yakashandurwa yenguva dzose mamorekuru biology kuedza sekushandura uye kugaya enzyme.
◮Bvisa RNA pasina kuwedzera RNase
◮Convenient-iyo centrifugation inoitwa pakamuri tembiricha
◮Fast-kushanda kunogona kupedzwa mumaminitsi makumi maviri
◮Safe-hapana organic reagent inoshandiswa
◮Kuchena kwepamusoro—OD260/280≈1.7-1.9
Kit Descriptions
Iyo FOREGENE'S nucleic acid kudhirowa uye yekuchenesa yakatevedzana zvigadzirwa zvinogona kutorwa nekucheneswa kune yepamusoro-mhando nucleic acids kubva kwakasiyana masosi uye yakaoma chikamu masampuli.Iyo DNA yekuwedzera yakatevedzana zvigadzirwa zvakavakirwa paDNA-Chete silica membrane yekuchenesa makoramu uye yakasarudzika fomula reagents inogona kuchenesa yemhando yepamusoro DNA kubva kune akasiyana masampuli.
Iyo General Plasmid Mini Kit inoshandisa DNA-Chete yekuchenesa column tekinoroji uye inoshanda SDS lysis formula yekuwana yepamusoro-mhando yeplasmid DNA kubva kubhakitiriya mukati memaminitsi makumi maviri.Iyo DNA-Chete silica gel membrane inoshandiswa muspin column chinhu chitsva chakasarudzika cheFOREGENE, chinogona kushambadza DNA zvine mutsindo uye zvakananga, uye kubvisa RNA, mapuroteni etsvina, maion uye mamwe maoga emukati mumaseru kusvika pamwero mukuru.Iyo yakanyanya kusunga inosunga yeplasmid DNA yeDNA-Chete yekuchenesa column ndeye 60μg DNA.Kuwanda kweplasmid DNA yakanyatsobudiswa inoenderana nehuwandu hwekugadzirisa kwebhakitiriya, nhamba yemakopi eplasmid (yakakwirira kopi, yakaderera kopi), matambudziko, tsika dzetsika nezvimwe zvinhu.
Zvinotsanangurwa
| General Plasmid Mini Kit | |||
| Kit content | DE-01001 | DE-01002 | DE-01003 |
| 50T | 100T | 250T | |
| Buffer S1 | 10ml | 20ml | 50ml |
| Buffer S2 | 10ml | 20ml | 50ml |
| Buffer S3 | 25ml | 50ml | 125ml |
| Buffer PW | 25ml | 50ml | 125ml |
| Buffer WB2 | 15ml | 30ml | 75ml |
| Buffer EB | 10ml | 20ml | 50ml |
| DNA-Chete Column | 50 | 100 | 250 |
| Manual | 1 | 1 | 1 |
Zvimiro&zvakanakira
- Hapana kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA muplasmid DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira kusvibiswa kwerabhoritari neiyo exogenous RNase.
-Kumhanyisa kumhanya: Plasmid DNA kubviswa kunogona kupedzwa mukati memaminitsi makumi maviri.
- Yakachengeteka: Hapana organic reagent kutorwa kunodiwa.
- Hunhu hwepamusoro: Iyo plasmid inowanikwa nekubvisa haina RNA, haina RNase uye yakanyanya kuderera ion yemukati, iyo inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
Kit application
Plasmid DNA kubviswa uye kucheneswa kweGram-negative mabhakitiriya.
Kuyerera kwebasa
Mufananidzo
Kuchengetedza uye hupenyu hwesherufu
Iyi kit inogona kuchengetwa kwemwedzi gumi nemaviri pasi pemamiriro akaoma pane tembiricha yekamuri (15-25 ℃);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa mu2–8℃.
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mu 37 ℃ mvura yekugezera kwemaminetsi gumi kunyungudutsa mvura, wosanganisa usati washandisa.
