Bhakitiriya DNA Isolation Kit Bacterial Genomic DNA Extraction Purification Kits
Kit Description:
Kurumidza kuchenesa yemhando yepamusoro genomic DNA kubva kubhakitiriya muchikamu chekukura kwelogarithmic.
◮Hapana RNase kusvibiswa:DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva mujenomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.
◮KumhanyaForegene Protease ine chiitiko chepamusoro kupfuura maprotease akafanana, uye inogaya masampula enyama nekukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me20-80 maminetsi.
◮Yakanakira:Iyo centrifugation inoitwa pakamuri tembiricha, uye hapana chikonzero che4 ° C yakaderera-tembiricha centrifugation kana ethanol kunaya kweDNA.
◮Safety:Hapana organic reagent extract inodiwa.
◮Hunhu hwepamusoro:Iyo yakabviswa genomic DNA ine zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
◮Micro-elution system:Inogona kuwedzera kuwanda kweiyo genomic DNA, iyo yakanakira kudzika kwerwizi kana kuyedza.
Tsanangudzo
Iyi kit inopa nzira inokurumidza uye iri nyore yekutora genomic DNA kubva kune tsika dzebhakitiriya (Gram-negative uye Gram-positive) kubva kune zvakasiyana-siyana;inogona kugadzirisa isingasviki 3ml yemaitiro ebhakitiriya muchikamu chekukura kwelogarithmic (1 × 109 mabhakitiriya).Iyo kit inofananidzwa nepamusoro-inoshanda Foregene Protease, iyo inobvumira 15-50μg yemhando yepamusoro genomic DNA kubviswa mukati meawa 1.Uye zvakare, iyo kiti inogona zvakare kubvisa genetic zvinhu kunze kweiyo genome, senge plasmids, Cosmid, BAC, nezvimwe.
Iyo DNA-chete silica gel matrix zvinhu inoshandiswa mu spin column chinhu chitsva chakasarudzika chekambani, chinogona kunyatso uye kunyanya adsorb DNA.Iyo yakanyanya adsorption kugona kweDNA i80μg.Iyo yakasarudzika buffer uye elution system inogona kukwirisa kubviswa kweRNA, Kusachena mapuroteni, ion uye mamwe organic komisheni mumasero.Iyo yakabviswa genomic DNA fragment yakakura, yakanyanya kuchena, yakagadzikana uye yakavimbika mhando, uye saizi yeDNA fragment yakagadzikana panenge 23kb.
Zvinotsanangurwa
50 Preps, 100 Preps, 250 Preps
Kit zvikamu
| Buffer ML1 |
| Buffer ML2 |
| Buffer PW |
| Buffer WB |
| Buffer EB |
| Foregene Protease |
| Lysozyme |
| Buffer TE |
| DNA-Chete Column |
| Mirayiridzo |
Zvimiro&zvakanakira
-Kwete kusvibiswa kweRNase: Hapana chikonzero chekuwedzera RNase kubvisa iyo RNA mune genomic DNA, kudzivirira murabhoritari kubva pakusvibiswa neiyo exogenous RNase.
-Kukurumidza kukurumidza: Foregene Protease ine chiitiko chepamusoro kupfuura maprotease akafanana uye inogona kugaya sampuli dzebhakitiriya dzakaputsika munguva pfupi.
-Kusununguka: Iyo centrifugation inoitwa pane tembiricha yekamuri, uye hapana chikonzero che4 ° C yakaderera-kupisa centrifugation kana ethanol kunaya kweDNA.
-Kuchengeteka: hapana organic reagent kutorwa kunodiwa.
-Hunhu hwepamusoro: Iyo yakabviswa genomic DNA zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, iyo inogona kusangana nezvinodiwa zvekuedza kwakasiyana.
Kit application
Iyi kit yakakodzera kuchenesa genomic DNA yemasampuli anotevera: Gram-negative bacteria uye Gram-positive bhakitiriya muchikamu chekukura kwelogarithmic.
Workflow
Storage uye Sherufu upenyu
-Iyi kititi inogona kuchengetwa kwemwedzi gumi nemaviri pasi pemamiriro akaoma pakushambidzika kwekamuri (15-25 ° C);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8°C.
Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.
-Forgene Protease mhinduro ine chimiro chakasiyana-siyana, iyo inoshanda kana yakachengetwa mukamuri yekushisa kwenguva yakareba (mwedzi mitatu);kana yakachengetwa pa4 ° C, basa rayo uye kugadzikana zvichava zviri nani, saka zvinokurudzirwa kuichengeta pa4 ° C, yeuka kuti urege kuichengeta pa -20 ° C.
-Lysozyme ipfu yakaoma, iyo inoshanda kana yakachengetwa pa 2-8 ° C kwenguva yakareba (mwedzi mitatu).Kuti uchengetedze kwenguva yakareba, tapota chengeta pa -20°C.
