Plant DNA Isolation Kit Genomic Plant DNA Kuchenesa Kits Reagents Protocol
Zvinotsanangurwa
50 Preps, 100 Preps, 250 Preps
Kit iyi inoshandisa DNA-chete column inogona kunyatsosunga DNA, Foregene protease uye yakasarudzika buffer system, iyo inorerutsa zvakanyanya kucheneswa kwechirimwa genomic DNA.Yepamusoro-mhando genomic DNA inogona kuwanikwa mukati me30 maminetsi, ayo anodzivirira kushatiswa kwegenomic DNA.
Iyo DNA-chete silica gel membrane inoshandiswa mu spin column ndeye Foregene's yakasarudzika nyowani zvinhu, iyo inogona zvinobudirira uye zvakananga kusunga kuDNA, uye kuwedzera kubviswa kweRNA, kusachena mapuroteni, ion, polysaccharides, polyphenols uye mamwe organic komisheni.
Zvigadzirwa zvechigadzirwa
| Buffer PL1, Buffer PL2 |
| Buffer PW, Buffer WB, Buffer EB |
| Foregene Protease |
| DNA-Chete Column |
| Mirayiridzo |
Zvimiro&zvakanakira
■ Hapana kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvikwanise kubvisa RNA kubva mujenomic DNA pasina imwe RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa nekunze kweRNase.
■ Kumhanya-mhanya: Foregene Protease ine chiitiko chepamusoro kupfuura maprotease akafanana uye inogaya masampuli enyama nekukurumidza.
■ Nyore: iyo genomic DNA yekubvisa oparesheni inogona kupedzwa mumaminitsi makumi matatu.
■ Yakanakira: Iyo centrifugation inoitwa pakamuri tembiricha, hapana 4 ℃ yakaderera-tembiricha centrifugation kana ethanol kunaya kweDNA kunodiwa.
■ Kuchengetedza: hapana organic reagent inodiwa.
■ Hunhu hwepamusoro: Iyo yakacheneswa genomic DNA ine zvimedu zvakakura, isina RNA, isina RNase, uye yakaderera zvakanyanya ioni, inogona kuzadzisa zvinodiwa pakuedza kwakasiyana.
Kit application
Inokodzera kubviswa uye kucheneswa kwegenomic DNA kubva kutsva kana chando matishu ezvirimwa.
Kuyerera kwebasa
Mufananidzo
Storage uye Sherufu upenyu
Iyo kit inogona kuchengetwa kwemwedzi gumi nemaviri pane tembiricha yekamuri (15-25 ℃) uye 2-8 ℃ kwenguva yakareba.
Foregene Protease Plus mhinduro ine yakasarudzika formula, inoshanda kana yakachengetwa pakamuri tembiricha kwenguva yakareba (mwedzi mitatu);basa rayo uye kugadzikana zvichava nani kana zvakachengetwa pa4 ℃, saka zvinokurudzirwa kuichengeta pa4 ℃, rangarira kusachengeta pa -20 ℃.
Dambudziko rekuongorora Guide
The following is an analysis of the problems that may be encountered in the extraction of plant genomic DNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mali: Tech@foregene.com.
Goho rakaderera kana pasina DNA
Kazhinji pane zvinhu zvakawanda zvinokanganisa goho rejenomic DNA, kusanganisira kunobva sampuli, zera remuenzaniso, mamiriro ekuchengetedza emuenzaniso, uye kushanda.
Genomic DNA haina kukwanisa kuwanikwa panguva yekubvisa
1. Matisue samples anochengetwa zvisina kunaka kana kuchengetwa kwenguva yakareba, zvichikonzera kuparara kwegenomic DNA.
Kurudziro: Chengeta masamples ematishu mune mvura nitrogen kana -20°C;edza kushandisa zvichangobva kuunganidzwa sampuli dzegenomic DNA kutorwa.
2. Yakanyanya kudikisa sampuli yemari inogona kukonzera inoenderana genomic DNA kuti isaburitswe.
Zano: Kumasampuli ematishu akachengetwa kwenguva yakareba kana ane yakanyanya genomic DNA degradation, huwandu hwemasampure ematissue hunogona kuwedzerwa nenzira kwayo kuitira kuburitsa yakawanda genomic DNA.Nhamba yemuenzaniso inogona kugadziriswa maererano nezvinodiwa zveDNA, asi mutsva mutsva haufaniri kudarika 100mg, uye sampuli yakaoma haifaniri kudarika 30mg.
3. Muenzaniso hausi pasi nenitrogen yemvura kana kuiswa kwenguva yakareba mushure memvura yenitrogen.
Zano: Panguva yekutorwa kweDNA, sampuli inoda kuve yakazara pasi nenitrogen yemvura kuti iparadze rusvingo rwesero;mushure mekugaya, ndapota tumira sampuli yehupfu kuPL1 preheated pa65°C nokukurumidza sezvinobvira (kana pasi poda yakanyunguduka, genomic DNA inotanga kuderera nokukurumidza) .
4. Kuchengetwa kusina kunaka kweForegene Protease kunoguma nekuderedzwa kana kusashanda basa.
Kurudziro: Simbisa mamiriro ekuchengetedza eForegene Protease kana kuitsiva neForegene Protease itsva ye enzymatic hydrolysis.
5. Iyo kiti inochengetwa zvisina kunaka kana kuchengetwa kwenguva yakareba, zvichiita kuti zvimwe zvikamu mukiti zvikundike.
Kurudziro: Tenga chirimwa chitsva genomic DNA yekubvisa kit yemabasa ane hukama.
6. Kushandiswa kusina kunaka kwekiti.
Zano: Tenga Plant DNA Isolation Kit yakatsaurirwa kune masampuli ekubvisa uye kucheneswa kwechirimwa genomic DNA.
7. Buffer WB pasina kuwedzera anhydrous ethanol.
Kurudziro: Ita shuwa kuti wawedzera iyo chaiyo vhoriyamu yeabsolute ethanol kuBuffer WB.
8. Eluent haina kudonhedzwa pa silica membrane nemazvo.
Zvaungaita: Wedzera pre-heated eluent pa65℃inodonha kusvika pakati pe silica gel membrane, uye woisiya iri pakamuri tembiricha kwemaminetsi mashanu kuti uwedzere kujekesa.
Kutora kuti uwane yakaderera-goho genomic DNA
1. Sample inochengetwa zvisina kunaka kana kuchengetwa kwenguva yakareba, zvichikonzera kuparara kwegenomic DNA.
Kurudziro: Chengetedza matishu samples pa -20℃;edza kushandisa zvichangobva kuunganidzwa matishu samples kuti genomic DNA kutorwa.
2. Kana huwandu hwemasikirwo emasikirwo ari mashoma, iyo yakabviswa genomic DNA ichava shoma.
Zano: Mamwe masampuli ezvirimwa akapfuma mumvura, senge zvirimwa zvemumvura zvakaita sealgae, zvichingodaro, dosi inogona kuwedzerwa zvakakodzera kana kuti mvura inogona kupera mvura shoma isati yaitwa.
3. Mienzaniso yakanga isina kunyatsogadzika nenitrogen yemvura kana kuti yakasiiwa patembiricha yekamuri kwenguva yakareba mushure mekukuya.
Zano: Iyo nitrogen yemvura inokuya inofanira kukwana, uye sampuli sero wall inofanira kuputswa zvakanyanya sezvinobvira;pakarepo mushure mekukuya, sampuli yehupfu inofanira kuendeswa ku65℃preheated Buffer PL1 yedanho rinotevera.
4. Kusashandisa kit chaiyo.
Kurudziro: Shandisa yakazvipira Plant DNA Isolation Kit kuburitsa uye kuchenesa chirimwa genomic DNA.
5. Kuchengetedzwa kwakashata kweForegene Protease kunoguma nekuderedzwa kana kusashanda basa.
Kurudziro: Simbisa mamiriro ekuchengetedza eForegene Protease kana kuitsiva neForegene Protease itsva ye enzymatic hydrolysis.
6. Eluent dambudziko
Kurudziro: Ndapota shandisa Buffer EB ye elution;kana uchishandisa ddH2O kana zvimwewo zvinonzwisiswa, ita shuwa kuti pH yeiyo eluent iri pakati pe7.0-8.5.
7. Rukudzo haruna kudonha nemazvo
Zano: Ndokumbirawo uwedzere kudonhedza kudonhedza pakati pe silica membrane uye woisiya iri pakamuri tembiricha kwemaminetsi mashanu kuti uwedzere kujekesa.
8. Vhoriyamu yakawandisa idiki
Zvaungaita: Ndokumbira ushandise iyo eluent ye genomic DNA elution zvinoenderana nemirairo, ingangoita isingasviki zana.μl.
Yakabviswa genomic DNA ine kuchena kwakaderera
Iyo yakaderera kuchena kweiyo genomic DNA ichaita kuti kukundikana kana kushata kwemaitiro ezasi ekuedza, senge: iyo enzyme haigone kutemwa, uye chinangwa chegene fragment hachigone kuwanikwa nePCR.
1. Miscellaneous protein kusvibiswa, RNA kusvibiswa.
Kuongorora: Buffer PW haina kushandiswa kushambidza mbiru;Buffer PW haina kushandiswa kushambidza mbiru pane chaiyo centrifugation kumhanya.
Zvaungaita: edza kuona kuti hapana kunaya kwemvura musimba repamusoro apo simba repamusoro rinopfuura nepakati;iva nechokwadi chekugeza koramu yekuchenesa neBuffer PW maererano nemirairo, uye danho iri harigoni kusiiwa.
2. Kusvibiswa ion kusvibiswa.
Ongororo: Iyo Buffer WB wash column yakasiiwa kana kungogezwa kamwe chete, zvichikonzera kusvibiswa kweionic kwasara.
Kurudziro: Iva nechokwadi chekugeza kaviri neBuffer WB maererano nemirairo yekubvisa maion akasara zvakanyanya sezvinobvira.
3. RNase kusvibiswa.
Kuongorora: Exogenous RNase inowedzerwa kuBuffer;Kushambidzika kwakashata muBuffer PW kunozokonzera RNase yakasara uye kukanganisa maekisesheni ekudzika kweRNA, senge in vitro transcription.
Zano: Foregene series nucleic acid extraction kits inogona kubvisa RNA pasina imwe RNase, uye mareagents ese muPlant DNA Isolation Kit haadi RNase;iva nechokwadi chekugeza koramu yekuchenesa neBuffer PW maererano nemirairo, uye danho iri harigoni kusiiwa.
4. Ethanol zvisaririra.
Ongororo: Mushure mekugeza koramu yekuchenesa neBuffer WB, hapana isina chinhu chubhu centrifugation yakaitwa.
Kurudziro: Tevedzera mirairo yekwakakodzera isina chinhu chubhu centrifugation.
Chinyorwa cheMirayiridzo:
Plant DNA Isolation Kit Instruction Manual
