Dyara Mbeu (yakakura nepakati-kati) Yakananga PCR Plus Kit II-UNG(isina Sampling Tools)
Tsanangudzo
Ichi chigadzirwa chinoshandisa yakasarudzika lysis reaction system, iyo inogona kukurumidza kuburitsa genomic DNA kubva kumiti yembeu samples ine yakakwira polysaccharides uye polyphenols (yakadai sebhanana, donje, nezvimwewo) kuti ishandiswe muPCR reactions, saka inonyanya kukodzera kune yakakura-yakakura genetic test.Kuti zvizadzise zvidikanwi zveongororo dzakasiyana-siyana, kit iyi inogona kushandisa mhando dzakawanda dzesampuli (sembeu dzizere, kucheka matishu madiki, kana sampuli dzepasi dzemhodzi dzakawanda) pakuedza.Pakati pavo, kune mbeu dzemasampuli dzichiri kuda kumera, unogona kusarudza kucheka zvimedu zviduku zviduku (1-5mg) kunze kwembeu embryo yekuedza;kune zviedzo zvinoda sampling uye kuyedzwa muhuwandu hukuru hwemasample, unogona kusarudza kusanganisa akawanda masampuli uye woasanganisa.Mushure mekugaya kuita zvidimbu zvine dhayamita ingangoita 0.5mm, kuyedza kunogona kuitwa (inenge 0.1% yeiyo inotarirwa sampu inogona kuonekwa).
Zvinotsanangurwa
50T,200T,500T,2000T
Kit zvikamu
Dyara Mbeu Yakananga PCR Plus Kit II-UNG Polysaccharide polyphenol chirimwa mbeu (yakakura uye yepakati-saizi) yakananga PCR kit-UNG | |||||
Kit composition | TP-0318T | TP-03181 | TP-03182 | TP-03183 | |
50 T | 200 T | 500 T | 2000 T | ||
Chikamu I | Buffer SSP1 | 24ml | 96ml | 120ml × 2 | 480ml × 2 |
Buffer SP2 | 3ml | 10ml | 25ml | 100ml | |
6 × DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml × 4 | |
Chikamu II | Buffer SSP2 | 8ml | 32ml | 80ml | 80ml × 4 |
2× Mbeu PCR EasyTM Mix | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml × 12 | |
Manual | 1 | 1 | 1 | 1 |
Zvimiro uye zvakanakira
- Hapana kutora nguva uye kudhura kweDNA kucheneswa.
- Sample inodiwa idiki, ingotora mhodzi imwe chete.
- Hapana kurapa kwakakosha senge kukuya nekupwanya kunodiwa, uye kushanda kuri nyore.
-Iyo yakagadziridzwa PCR sisitimu inoita kuti PCR ive nemhando yepamusoro uye yakasimba kushivirira kune PCR reaction inhibitors..
- Anti-kusvibisa PCR system 2 × Mbeu PCR NyoreTM Sanganisa (UNG), iyo inogona kunyatsobvisa kusvibiswa kunokonzerwa nePCR zvigadzirwa uye kuve nechokwadi cheiyo chaiyo uye nekururama kweamplification.
Workflow
Kuchengetedza mamiriro
1.Kutakurwa
Iyo yese maitiro ekudzika-tembiricha echando bhokisi chekufambisa, kuve nechokwadi chekuti kit Chikamu II chiri mumamiriro e <4 ℃.
2.Kuchengeta
Chikamu I chekiti ichi chinofanira kuchengetwa mukamuri tembiricha kana 2-8 ° C.
Chikamu II chinofanira kuchengetwa pa -20°C.