RT-PCR Easyᵀᴹ II(Nhanho mbiri)
Kit Description:
◮Real-time quantitative analysis yeRNA inogona kuitwa nekukurumidza uye nemazvo.
◮Iyo kit inoshandisa yakasarudzika yeForegene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
◮ Iyo optimized reaction system inoita kuti mhinduro ive nepamusoro yekuona senitivity, kusimba kwekupisa kwekushisa, uye kushivirira kuri nani.
Zvinotsanangurwa
RT-PCR NyoreTM II(Nhanho mbiri) ine ese mareagents maviri-nhanho RT-PCR, achibatanidza RT maitiro uye PCR maitiro mune imwechete kit, ichipa yakagadziridzwa RT Musanganiswa uye PCR Musanganiswa.Iyo yakasarudzika RT Musanganiswa inogona kubatanidza yakakwirira-yemhando yekutanga-strand cDNA zviri nyore uye zvinobudirira. 2× PCREasyTM MixUyezve ine hunyanzvi hwepamusoro uye yakananga amplification.Iyo organic musanganiswa wezviviri izvi inoita kuti kuoneswa kwejini rinotangwa kuve nehanya.
Kit zvikamu
| RT-PCREasyTM II (Nhanho mbiri) | ||
| Kit zviri mukati | RT-02021 | RT-02022 |
| 100T(20μlsystem) | 500T(20μlsystem) | |
| 2 × RT NyoreTM Mix | 1ml | 1.7ml×3 |
| 2× PCREasyTM MixUyezve | 1ml | 1.7ml×3 |
| Random Primer (50μM) | 200μl | 1ml |
| Oligo(dT)18 Primer (50μM) | 200μl | 1ml |
| RNase-Yemahara ddH2O | 1.7ml | 1.7ml |
| Bhuku reMirayiridzo | 1chidimbu | 1chidimbu |
Zvimiro&zvakanakira
-Real-time quantitative analysis yeRNA inogona kuitwa nekukurumidza uye nemazvo.
-Iyo kit inoshandisa yakasarudzika yeForegene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
-Iyo optimized reaction system inoita kuti mhinduro ive nepamusoro yekuona senitivity, kusimba kwekupisa kwekushisa, uye kushivirira kuri nani.
Workflow
Storage uye Sherufu upenyu
1.Mamiriro ekufambisa
Iyo yese maitiro ekudzika-tembiricha echando bhokisi chekufambisa, kuve nechokwadi chekuti kit iri mumamiriro e <4 °C.
2. Mamiriro ekuchengetedza
Iyo kit inochengetwa pa -20 ° C.Chengetedza chigadzirwa mufiriji yekushisa nguva dzose pa -20 ° C pakarepo mushure mekugamuchira.Kana mamiriro ekuchengetedza akakodzera,tiye chigadzirwa hachizodzikisi chero mashandiro panguva ye1-gore yechokwadi nguva.
