1kb DNA Ladder
Kit Description:
◮Iyo 1Kb DNA Ladder inoumbwa ne13 tambo mbiri dzeDNA, uye iyo yakapetwa kaviri ye1kb uye 5kb inobatsira kusiyanisa mabhendi akasiyana.Pazasi pe1kb, mabhendi matatu e250bp, 500bp, uye 750bp anowedzerwa, uye saizi yechidimbu chipfupi inogona kuverengerwa kuverengerwa zvichienderana neizvi.Iko kusungirirwa kwemabhandi mana e6kb, 7kb, 8kb, uye 10kb yakaganhurwa, izvo zvinobatsira kuwana kupatsanurwa kuri nani.
Tsanangudzo
Iyo 1Kb DNA Ladder inoumbwa ne13 tambo mbiri dzeDNA, uye iyo yakapetwa kaviri ye1kb uye 5kb inobatsira kusiyanisa mabhendi akasiyana.Pazasi pe1kb, mabhendi matatu e250bp, 500bp, uye 750bp anowedzerwa, uye saizi yechidimbu chipfupi inogona kuverengerwa kuverengerwa zvichienderana neizvi.Iko kusungirirwa kwemabhandi mana e6kb, 7kb, 8kb, uye 10kb yakaganhurwa, izvo zvinobatsira kuwana kupatsanurwa kuri nani.
Ichi chigadzirwa chatova ne1 × Kurodha Buffer.Iwe unogona kutora 2-6μl kune yakananga electrophoresis maererano nezvinodiwa zvekuedza.Mufananidzo we electrophoresis wakajeka, wakanakira uye unoshanda.
Mabhendi gumi nematatu echigadzirwa ichi kubva kudiki kusvika kuhombe ndeaya: 250bp, 500bp, 750bp, 1kb, 1.5kb, 2kb, 3kb, 4kb, 5kb, 6kb, 7kb, 8kb, 10kb.Kana vhoriyamu yekurodha iri 5μl, 1kb uye 5kb mabhendi ari 100ng;6kb, 7kb, 8kb, uye 10kb mabhendi ane 25ng;uye mamwe mabhande asara 50ng.
Zvinotsanangurwa
| DL-30011 | 50 Preps (250μl) |
| DL-30012 | 200 Preps (1ml) |
Kit zvikamu
Ruzivo rwemvura yekuchengetera
10mM Tris-HCl(pH 8.4) 10mM EDTA
0.02% BPB
5% glycerinum
Product concentration
Chigadzirwa chekuisa chinenge 130μg/ml
Electropherogram
Kuchengetedza mamiriro
Ndapota chengeta pa4℃(Ndokumbira uchengete pa -20℃kuchengetedza kwenguva refu)
