RT-PCR Easyᵀᴹ I (Imwe Danho)
Kit Description:
◮Iyo imwe-nhanho kit inogonesa reverse transcription uye PCR kuti iitwe muhubhu imwechete.Inongoda kuwedzera template RNA, chaiyo PCR primers uye RNase-Yemahara ddH2O.
◮Real-time quantitative analysis yeRNA inogona kuitwa nekukurumidza uye nemazvo.
◮Iyo kit inoshandisa yakasarudzika yeForegene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
◮Iyo optimized reaction system inoita kuti mhinduro ive nepamusoro yekuona senitivity, kusimba kwekupisa kwekushisa, uye kushivirira kuri nani.
Zvinotsanangurwa
Foregene Imwe-Nhanho RT-PCR NyoreTM Zvigadzirwa zvakatevedzana zvinoona maitiro akabatanidzwa kubva kuRNA kuenda kune yakapetwa kaviri DNA, kureva, reverse transcription uye PCR amplification inopedzwa mune imwechete reaction centrifuge chubhu uye yakafanana reaction system, iyo inorerutsa matanho ekuyedza., optimizestiye experimental chirongwa, uyeinovandudza kuedza kunyatsoshanda.
RT-PCR NyoreTM I(Imwe Danho) inhanho imwe RT-PCR kit yakanyatso gadzirwa neForegene, iyo inoziva kuenderera mberi kwekushanda kubva kuRT kuenda kuPCR mune imwechete chubhu.Iko kushanda kuri nyore uye nekukurumidza, izvo zvinogona kunyatso kudzikisa kusvibiswa panguva yekuedza.2×RT-PCR NyoreTM Musanganiswa une kushivirira kwakasimba, kugadzikana kwekupisa, kushanda kwakanyanya uye kwakanangaity for amplification.
Kit zvikamu
| RT-PCR NyoreTMIni (Imwe Danho) | ||
| Kit zviri mukati | RT-02011 | RT-02012 |
| 100T (20μl system) | 500T (20μl system) | |
| 2 × RT-PCR NyoreTM Mix | 1ml | 1.7ml×3 |
| RNase-Yemahara ddH2O | 1.7ml | 1.7ml×3 |
| Bhuku reMirayiridzo | 1 chidimbu | 1chidimbu |
Zvimiro&zvakanakira
-Iyo nhanho-imwe kit inogonesa reverse transcription uye PCR kuti iitwe muhubhu imwechete.Inongoda kuwedzera template RNA, chaiyo PCR primers uye RNase-Yemahara ddH2O.
-Chaiyo-nguva yehuwandu yekuongorora yeRNA inogona kuitwa nekukurumidza uye nemazvo.
-Iyo kit inoshandisa yakasarudzika Foregene reverse transcription reagent uye Foregene HotStar Taq DNA Polymerase yakasanganiswa neyakasarudzika maitiro sisitimu kuti inyatso kunatsiridza kugona kwekusimudzira uye kujeka kwemaitiro.
- Iyo yakagadziridzwa maitiro system inoita kuti maitiro ave nepamusoro ekuona senitivity, kusimba kwekushisa kwekushisa, uye kushivirira kuri nani.
Workflow
Storage uye Sherufu upenyu
1.Mamiriro ekufambisa
Iyo yese maitiro ekudzika-tembiricha echando bhokisi chekufambisa, kuve nechokwadi chekuti kit iri mumamiriro e <4 °C.
2. Mamiriro ekuchengetedza
Iyo kit inochengetwa pa -20 ° C.Chengetedza chigadzirwa mufiriji yekushisa nguva dzose pa -20 ° C pakarepo mushure mekugamuchira.Kana mamiriro ekuchengetedza akakodzera,tiye chigadzirwa hachizodzikisi chero mashandiro panguva ye1-gore yechokwadi nguva.
