Isu tave neruzivo mugadziri.Kuhwina ruzhinji rwezvakakosha zvitupa zvemusika wayo weSuper Purchasing yeChina Chaiyo-Nguva PCR, Kana iwe uchifarira chingangoita chero zvinhu, rangarira kunzwa wakasununguka zvachose kuti ubatwe nesu kuti uwane rumwe ruzivo kana ive shuwa kutipa email chaiko, tichakupindura mumaawa makumi maviri nemana chete pamwe nekotesheni yakanaka kwazvo ichapihwa.
Isu tave neruzivo mugadziri.Kuhwina ruzhinji rwezvako zvakakosha zvitupa zvemusika wayoChina PCR, Nguva Yechokwadi Pcr, Nemweya unokatyamadza we "kushanda kwepamusoro, kurerutsira, kuita uye hunyanzvi", uye zvinoenderana nekutungamira kwakadaro kwe "mhando yakanaka asi mutengo uri nani," uye "chikwereti chepasi rose", tiri kuedza kushandirapamwe nemakambani ezvikamu zvemotokari pasi rese kuita mubatanidzwa wekuhwina.

Tsanangudzo

Iyi kit inoshandisa yakasarudzika lysis buffer sisitimu inogona kukurumidza kuburitsa RNA kubva kune yakasimwa maseru masampula eRT-qPCR maitiro, nekudaro ichibvisa iyo inopedza nguva uye yakaoma RNA yekuchenesa maitiro.Iyo RNA template inogona kuwanikwa mumaminetsi manomwe chete.Iyo 5 × Direct RT Musanganiswa uye 2 × Direct qPCR Musanganiswa-SYBR reagents anopihwa nekiti anogona nekukurumidza uye zvinobudirira kuwana chaiyo-nguva yehuwandu hwePCR mhinduro.

5 × Yakananga RT Mix uye 2 × Yakananga qPCR Mix-SYBR ine inhibitor kushivirira kwakasimba, uye lysate yemasampuli inogona kushandiswa se template yeRT-qPCR zvakananga.Iyi kit ine yakasarudzika RNA yakakwirira-yakabatana Foregene reverse transcriptase, uye Inopisa D-Taq DNA polymerase, dNTPs, MgCl.₂, reaction buffer, PCR optimizer uye stabilizer.

Zvinotsanangurwa

200×20μl Rxns, 1000×20μl Rxns

Kit zvikamu

Zvimiro&zvakanakira

■ Zviri nyore uye zvinoshanda : neTekinoroji yeCell Direct RT, masampuli eRNA anogona kuwanikwa mumaminetsi manomwe chete.

∎ Sample kudiwa idiki, yakaderera semaseru gumi anogona kuongororwa.

■ High throughput: inogona kukurumidza kuona RNA mumaseru akagadzirwa mu384, 96, 24, 12, 6-tsime mahwendefa.

∎ DNA Eraser inogona kukurumidza kubvisa majenomes akaburitswa, kuderedza zvakanyanya kukanganiswa pamhedzisiro yekuedza.

■ Optimized RT uye qPCR sisitimu inoita kuti nhanho mbiri RT-PCR reverse transcription inyatsoita uye PCR yakanyatsojeka, uye inoshingirira kuRT-qPCR reaction inhibitors.

Kit application

Kuwanda kwekushandiswa: masero akagadzirwa.

- RNA yakaburitswa nesample lysis: inoshanda chete kune RT-qPCR template yeiyi kit.

- Iyo kit inogona kushandiswa kune zvinotevera zvinangwa: gene kutaura kuongororwa, kuoneswa kweSiRNA-mediated gene kunyaradza maitiro, kuongorora zvinodhaka, nezvimwe.

Mufananidzo

Storage uye Sherufu upenyu

Chikamu I chekiti ichi chinofanira kuchengetwa pa4 ℃;Chikamu II chinofanira kuchengetwa pa -20 ℃.

Foregene Protease Plus II inofanira kuchengetwa pa 4 ℃, isaome pa -20 ℃.

Reagent 2 × Direct qPCR Mix-SYBR inofanira kuchengetwa pa -20 ℃ murima;kana ikashandiswa kakawanda, inogonawo kuchengetwa pa4 ℃ kuitira kuchengetedza kwenguva pfupi (shandisa mukati memazuva gumi) .Tave tine ruzivo rwekugadzira.Kuhwina ruzhinji rwezvakakosha zvitupa zvemusika wayo weSuper Purchasing yeChina Q160/Q320 Chaiyo-Nguva PCR, Kana iwe uchifarira chingangoita chero zvinhu, rangarira kunzwa wakasununguka zvachose kuti utaure nesu kuti uwane zvimwe chokwadi kana kuve neshuwa kutipa email chaiko, isu tichakupindura iwe mumaawa makumi maviri nemana chete pamwe nekotesheni ichapihwa.
Super Kutenga kweChina PCR, Real Time PCR, Nemweya unoshamisira we” kushanda kwepamusoro, kurerutsira, kuita uye hunyanzvi”, uye zvinoenderana negwara rekushandira rakadai re "mhando yakanaka asi mutengo uri nani," uye "chikwereti chepasi rose", tiri kuedza kushandirapamwe nemakambani ezvikamu zvemotokari pasi rese kuita mubatanidzwa wekuhwina.