Pamwe chete nehunyanzvi hwekambani ye "Client-Oriented", chirongwa chakaomarara chemhando yepamusoro, giya rekugadzira rakasimba uye vashandi vakasimba veR&D, isu tinogara tichiunza mhinduro dzemhando yepamusoro, zvigadzirwa zvemhando yepamusoro uye masevhisi uye hukasha hwemitengo yeNew Arrival China Genomic DNA Extraction Kit (Ropa/Mate) inoita kuti isu tisarudze makwikwi aya uye tinovimba kuti isu takasiyana nemakwikwi aya.Isu tese tinoshuvira kuita kuhwina-kuhwina zvibvumirano nevatengi vedu, saka tipei kuonana nhasi uye gadzira shamwari itsva!
Pamwe chete nekambani ye "Client-Oriented" huzivi hwekambani, chirongwa chakasimba chepamusoro-soro, giya rekugadzira uye rakasimba reR&D vashandi, isu tinogara tichipa zvigadziriso zvemhando yepamusoro, zvigadzirwa zvemhando yepamusoro uye masevhisi uye huwandu hwemitengo yehukasha, Zvinhu zvedu zvakawanikwa zvakanyanya kuzivikanwa kubva kune vatengi vekunze, uye nekumisikidzwa nehukama hurefu nekubatana navo.Tichapa sevhisi yakanakisa kune wese mutengi uye tinogashira nemoyo shamwari shamwari kuti dzishande nesu uye kumisikidza kubatsirana pamwe chete.

Kit Descriptions

Iyi kit inotora itsva Foregene Protease Plus uye yakasarudzika BL1 uye BL2 buffer masisitimu, anogona kugaya zvachose anticoagulant masampu eropa munguva pfupi, nekudaro kudzivirira kuderedzwa kweDNA kusvika padanho repamusoro uye kuwana huwandu hwepamusoro hwegenomic DNA.Iyo yekukurumidza kugadzirisa ropa system uye iri nyore DNA-Chete column operation inorerutsa zvakanyanya kutorwa kweropa genome, kuita iyo genomic DNA inogona kuwanikwa yemhando yepamusoro uye kuchena kwepamusoro mukati memaminitsi makumi mana.

Iyo DNA-chete silica gel membrane inoshandiswa mumutsara wecentrifuge wekiti iyi rudzi rutsva rwezvinhu zvakasiyana nekambani yedu, iyo inogona kusunga DNA zvakanyatsonaka uye zvakananga, uye inogona kubvisa RNA, tsvina, mapuroteni, ions uye mamwe organic compounds mumasero kusvika pamwero mukuru.Izvo zvakawanikwa zvidimbu zveDNA zvakakura, kuchena kwepamusoro, kugadzikana uye kuvimbika kwemhando.Hukuru hwekutakura huwandu hweDNA-Chete column ndeye 80 μg DNA.Iyo yakawanikwa DNA inogona kushandiswa mumamolecular biology kuyedza senge enzyme digestion, PCR, Southern hybridization uye kuvaka raibhurari.

Iyo kit inogona kugadzira kusvika ku1ml yeropa panguva kuti iwane huwandu hwakakwana hwepamusoro-kuchena genomic DNA.

Zvinotsanangurwa

*: Buffer BL2 uye Buffer PW ine inovava mvura yemunyu.Ndokumbira upfeke magirovhosi uye tora matanho ekudzivirira akakodzera paunenge uchishanda.

Zvimiro&zvakanakira

-Kwete kusvibiswa kweRNase: Iyo DNA-Chete Column yakapihwa nekiti inoita kuti zvibvire kubvisa RNA kubva kune genomic DNA pasina kuwedzera RNase panguva yekuyedza, kudzivirira rabhoritari kuti isasvibiswa neRNase yekunze.

-Kukurumidza kukurumidza: Foregene Protease Plus ine basa rakakwirira kudarika maproteases akafanana, uye inochera sampuli dzenyama nokukurumidza;kuvhiya kuri nyore, uye genomic DNA yekubvisa oparesheni inogona kupedzwa mukati me40 maminetsi.

-Kusununguka: Iyo centrifugation inoitwa pakupisa kwekamuri, 4 ° C centrifugation kana ethanol precipitation yeDNA haidiwi.

-Kuchengeteka: Hapana organic reagent kutorwa kunoshandiswa.

-Hunhu hwepamusoro: Iyo yakabviswa genomic DNA zvimedu zvakakura, hapana RNA, hapana RNase, uye yakanyanya kuderera ion yemukati, iyo inogona kusangana nezvinodiwa zvekuedza kwakasiyana.

Kit application

Inokodzera kubviswa uye kucheneswa kwegenomic DNA kubva kutsva kana chando anticoagulated ropa rose.

Kuyerera kwebasa

Kuchengetedza uye hupenyu hwesherufu

Iyi kit inogona kuchengetwa kwemwedzi gumi nemiviri pasi pemamiriro akaoma panzvimbo yekupisa (15-25 ° C);kana ichida kuchengetwa kwenguva yakareba, inogona kuchengetwa pa2-8°C.

Ongorora: Kana yakachengetwa pane tembiricha yakaderera, mhinduro yacho inonangana nekunaya.Usati washandisa, iva nechokwadi chekuisa mhinduro mukiti pane tembiricha yekamuri kwenguva yakati.Kana zvichidikanwa, preheat mubhati yemvura ye 37 ° C kwemaminitsi gumi kuti iparadze mvura, uye isanganise isati yashandiswa.

Iyo Foregene Protease Plus mhinduro ine yakasarudzika formula, inoshanda kana yakachengetwa mukamuri tembiricha kwenguva yakareba (3 mwedzi);ichave yakanyanya kushanda uye yakagadzikana kana yakachengetwa pa4 ° C, saka inokurudzirwa kuichengeta pa4 ° C, yeuka kuti usaichengeta pa -20 ° C.

Pamwe chete ne "Client-Oriented" yekambani uzivi, yakasimba-yemhando yepamusoro dhizaini chirongwa, yakaomesesa kugadzira giya uye yakasimba R&D vashandi, isu tinogara tichipa emhando yepamusoro mhinduro, zvigadzirwa zvemhando yepamusoro uye masevhisi uye hukasha hwemitengo yeNew Arrival China Genomic DNA Extraction Kit (Ropa/Mate) isu tinotenda kuti vanovimba nemakwikwi aya.Isu tese tinoshuvira kuita kuhwina-kuhwina zvibvumirano nevatengi vedu, saka tipei kuonana nhasi uye gadzira shamwari itsva!
New Arrival China, Zvinhu zvedu zvakawanikwa zvakanyanya uye zvakanyanya kucherechedzwa kubva kune vekunze vatengi, uye zvakamiswa kwenguva refu uye hukama hwekubatana navo.Tichapa sevhisi yakanakisa kune wese mutengi uye tinogashira nemoyo shamwari shamwari kuti dzishande nesu uye kumisikidza kubatsirana pamwe chete.