Yakanakisa Kutanga, uye Consumer Supreme ndiyo nhungamiro yedu yekuendesa masevhisi epamusoro kune vatengi vedu. Mazuva ano, tiri kuedza nepatinogona napo kuti tive pakati pevanotengesa kunze kwenyika muindasitiri yedu kusangana nevatengi zvakanyanya kudiwa kweOEM Factory yePamusoro Kuvimbika Rt-Qpcr 2X Imwe-Nhanho Multiplex Master Mix, Isu tinovimbisa kuyedza yedu huru kukuunzirai zvigadzirwa zvemhando yepamusoro uye zvehupfumi zvemhando yepamusoro.
Zvakanakisa Kutanga, uye Consumer Supreme ndiyo nhungamiro yedu yekuunza masevhisi epamusoro kune vatengi vedu. Mazuva ano, tiri kuedza nepatinogona napo kuti tive pakati pevanotengesa kunze muindasitiri yedu kusangana nevatengi zvakanyanyaChina Taq DNA Polymerase uye Qpcr, Nebasa repamusoro uye rakasiyana, takagadziridzwa pamwe chete nevatengi vedu.Unyanzvi uye kuziva-kuita shuwa kuti isu tinogara tichinakidzwa neruvimbo kubva kune vatengi vedu mumabasa edu ebhizinesi."Hunhu", "kutendeseka" uye "basa" ndiwo musimboti wedu.Kuvimbika kwedu uye zvitsidzo zvinoramba zvine ruremekedzo pabasa rako.Bata Isu Nhasi Kuti uwane rumwe ruzivo, taura nesu izvozvi.
Real Time PCR primer dhizaini misimboti

Pamberi Primer uye Reverse Primer

Kune Real Time PCR, dhizaini yekutanga yakakosha.MaPrimers ane hukama nehunyanzvi uye kugona kwePCR amplification, uye inogona kugadzirwa ichitarisa kune anotevera misimboti:

• Kureba kwekutanga: 18-30bp.
• GC yemukati: 40-60%.
• Tm kukosha: Primer dhizaini software, senge Primer 5, inogona kupa iyo Tm kukosha kweiyo primer.Iyo Tm tsika dzekumusoro uye pasi pemvura dzekutanga dzinofanira kunge dziri padyo sezvinobvira.The Tm calculation formula inogonawo kushandiswa: Tm = 4 °C (G + C) + 2 °C (A + T).Paunenge uchiita PCR, tembiricha iri pasi peiyo primer Tm kukosha kwe5 ° C inowanzo sarudzwa seye tembiricha yekudziya (iyo inowirirana kuwedzera kwekushisa kweanona inogona kuwedzera iyo chaiyo yePCR reaction).
• Primers uye PCR zvigadzirwa:

1. Dhizaini primer PCR yekusimudzira chigadzirwa kureba ingangoita 100-150bp.
2. Dhizaini yekutanga munzvimbo yechipiri yemaitiro eiyo template inofanira kudziviswa zvakanyanya sezvinobvira.
3. Dzivisa kuumbwa kwe 2 kana anopfuura mabhesi anowirirana pakati pe 3′ migumo yekumusoro nekudzika kwemvura.
4. Primer 3′ terminal base haigone kuvepo ne3 yekuwedzera yakatevedzana G kana C.
5. Iwo maprimer pachawo haagone kuve neanopindirana zvimiro, zvikasadaro chimiro chebvudzi chinoumbwa, chinokanganisa PCR amplification.
6. ATCG inofanirwa kugovaniswa zvakaenzana sezvinobvira mukutevedzana kwekutanga, uye 3 ′ terminal base inofanira kudzivirirwa seT.

Appendikisi1:Cuye DirectRT-qPCR Kit component supplement pack

1.Cell Lysis Solution

 Yakanakisa Kutanga, uye Consumer Supreme ndiyo nhungamiro yedu yekuendesa masevhisi epamusoro kune vatengi vedu. Mazuva ano, tiri kuedza nepatinogona napo kuti tive pakati pevanotengesa kunze kwenyika muindasitiri yedu kusangana nevatengi zvakanyanya kudiwa kweOEM Factory yePamusoro Kuvimbika Rt-Qpcr 2X Imwe-Nhanho Multiplex Master Mix, Isu tinovimbisa kuyedza yedu huru kukuunzirai zvigadzirwa zvemhando yepamusoro uye zvehupfumi zvemhando yepamusoro.
OEM Factory yeChina Taq DNA Polymerase uye Qpcr, Nebasa repamusoro uye rakasiyana, takagadziridzwa pamwe chete nevatengi vedu.Unyanzvi uye kuziva-kuita shuwa kuti isu tinogara tichinakidzwa neruvimbo kubva kune vatengi vedu mumabasa edu ebhizinesi."Hunhu", "kutendeseka" uye "basa" ndiwo musimboti wedu.Kuvimbika kwedu uye zvitsidzo zvinoramba zvine ruremekedzo pabasa rako.Bata Isu Nhasi Kuti uwane rumwe ruzivo, taura nesu izvozvi.

Real Time PCR primer dhizaini misimboti

Pamberi Primer uye Reverse Primer

Kune Real Time PCR, dhizaini yekutanga yakakosha.MaPrimers ane hukama nehunyanzvi uye kugona kwePCR amplification, uye inogona kugadzirwa ichitarisa kune anotevera misimboti:

• Kureba kwekutanga: 18-30bp.
• GC yemukati: 40-60%.
• Tm kukosha: Primer dhizaini software, senge Primer 5, inogona kupa iyo Tm kukosha kweiyo primer.Iyo Tm tsika dzekumusoro uye pasi pemvura dzekutanga dzinofanira kunge dziri padyo sezvinobvira.The Tm calculation formula inogonawo kushandiswa: Tm = 4 °C (G + C) + 2 °C (A + T).Paunenge uchiita PCR, tembiricha iri pasi peiyo primer Tm kukosha kwe5 ° C inowanzo sarudzwa seye tembiricha yekudziya (iyo inowirirana kuwedzera kwekushisa kweanona inogona kuwedzera iyo chaiyo yePCR reaction).
• Primers uye PCR zvigadzirwa:

1. Dhizaini primer PCR yekusimudzira chigadzirwa kureba ingangoita 100-150bp.
2. Dhizaini yekutanga munzvimbo yechipiri yemaitiro eiyo template inofanira kudziviswa zvakanyanya sezvinobvira.
3. Dzivisa kuumbwa kwe 2 kana anopfuura mabhesi anowirirana pakati pe 3′ migumo yekumusoro nekudzika kwemvura.
4. Primer 3′ terminal base haigone kuvepo ne3 yekuwedzera yakatevedzana G kana C.
5. Iwo maprimer pachawo haagone kuve neanopindirana zvimiro, zvikasadaro chimiro chebvudzi chinoumbwa, chinokanganisa PCR amplification.
6. ATCG inofanirwa kugovaniswa zvakaenzana sezvinobvira mukutevedzana kwekutanga, uye 3 ′ terminal base inofanira kudzivirirwa seT.

Appendikisi1:Cuye DirectRT-qPCR Kit component supplement pack

1.Cell Lysis Solution

Mirayiridzo yezvinyorwa:

 Kurumidza Nyore Sero Yakananga RT-qPCR Kit-Taqman